A method for preparing soybean root exudates that efficiently promotes soybean rhizobia to compete for nodulation
A technology of soybean rhizobia and root exudates, applied in the field of bioengineering, can solve the problems of restricting the in-depth research, the effectiveness cannot be evaluated, and the components and concentrations are unknown, so as to achieve the effect of increasing expression and efficiently promoting competitive nodulation
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[0023] Example 1: A method for preparing soybean root exudates that efficiently promotes soybean rhizobia to compete for nodulation:
[0024] (1), the soybean seeds are first washed with distilled water, soaked in 95% alcohol for 1 min, then soaked in 5% sodium hypochlorite solution for 5 min for surface sterilization, and then rinsed with sterile water for at least 10 times;
[0025] (2), move the surface-sterilized soybean seeds to the YMA agar watch dish for germination under aseptic conditions, and after the seeds germinate, move to the sterilized nitrogen-free Rigaud-Puppo nutrient solution of 30% for cultivation , the soybean root exudates were collected on the 12th day of cultivation, and the soybean root exudates that could effectively promote the nodulation competition of soybean rhizobia were obtained; the cultivation conditions were: 30°C during the day, 16h light, and the light intensity was 960μmol·m -2 ·s -1 ; Night 15 ℃, 8h dark.
[0026] The beneficial effe...
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