A kind of amantadine artificial hapten, artificial antigen and its preparation method and application

An artificial hapten, amantadine technology, applied in the preparation of carboxylic acid amides, chemical instruments and methods, preparation of organic compounds, etc. Good sex, improve immune characteristics, good specificity

Active Publication Date: 2016-04-06
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there are no related reports on amantadine artificial hapten and artificial antigen

Method used

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  • A kind of amantadine artificial hapten, artificial antigen and its preparation method and application
  • A kind of amantadine artificial hapten, artificial antigen and its preparation method and application
  • A kind of amantadine artificial hapten, artificial antigen and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] This implementation of a preparation method of amantadine artificial antigen (the reaction process is as figure 1 ), including the following steps:

[0056] (1) Preparation of artificial haptens:

[0057] ① Weigh 200mg amantadine (1.322mmol) and 198mg succinic anhydride (1.980mmol) in a 100ml single-necked round bottom flask, add 10ml pyridine, add a stir bar, heat the oil bath to 100°C and stir reflux for 16 hours; react After the end, the solvent was evaporated under reduced pressure. The residue was dissolved in 15ml double-distilled water. The aqueous solution was extracted with 3×15ml dichloromethane. The organic phases were combined and washed with 15ml double-distilled water. The organic phase was dried over anhydrous magnesium sulfate, filtered, and transferred. After drying, 252 mg of colorless oily product A is obtained;

[0058] The colorless oily product A was subjected to TLC detection, and the chromatographic solution was an aqueous ethanol solution with a conce...

Embodiment 2

[0075] This implementation of a preparation method of amantadine artificial antigen (the reaction process is as figure 1 ), including the following steps:

[0076] (1) Preparation of artificial haptens:

[0077] ①Weigh 200mg amantadine (1.322mmol) and 145.6mg succinic anhydride (1.455mmol) in a 100ml single-necked round bottom flask, add 10ml pyridine, add a stir bar, heat the oil bath to 105°C and stir reflux for 17 hours; After the reaction, the solvent was evaporated under reduced pressure. The residue was dissolved in 15ml of double-distilled water. The aqueous solution was extracted with 3×15ml of dichloromethane. The organic phases were combined and washed with 15ml of double-distilled water. The organic phase was dried over anhydrous magnesium sulfate and filtered. After drying, 251.5mg of colorless oily product A is obtained;

[0078] The colorless oily product A was subjected to TLC detection, and the chromatographic solution was an aqueous ethanol solution with a concentra...

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Abstract

The invention discloses an amantadine artificial hapten, artificial antigen, a preparation method and application thereof. The molecular structural formula of the amantadine artificial hapten is shown in formula (I), and the molecular structural formula of the amantadine artificial antigen is shown in formula (II). The application is the application of the amantadine artificial antigen in the preparation of anti-amantadine antibody. The amantadine artificial hapten of the present invention retains the characteristic structure of amantadine to the greatest extent, and has an active group that can be coupled with a carrier protein, which can be used as an antigenic determinant; the obtained amantadine artificial antigen is further prepared The anti-amantadine antibody with high affinity, high sensitivity and strong specificity can be obtained by immunization, and the titer of the immune serum obtained by immunizing New Zealand white rabbits is as high as 1:70000, which can be used for rapid and accurate immunological detection and detection of amantadine immunoassay.

Description

Technical field [0001] The invention belongs to the field of biochemical technology, and specifically relates to an artificial hapten of amantadine, an artificial antigen, and a preparation method and application thereof. Background technique [0002] Amantadine can inhibit the virus from penetrating into host cells, affect the uncoating of the virus, and inhibit its reproduction. It is clinically used to prevent and treat human influenza virus infection. It has the characteristics of low price and obvious efficacy. It has also been Treatment drugs for Parkinson's syndrome. But the accumulation of amantadine can cause drowsiness, insomnia, dizziness, depression, nausea, loss of appetite and other adverse reactions to the human body, and has certain toxic and side effects. Long-term use of amantadine can also cause the virus to produce different degrees of drug resistance and induce the production of new drug-resistant viruses. [0003] However, in November 2012, the media reveale...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C233/47C07C231/02C07K14/47C07K16/44G01N33/53
Inventor 杨华邵越水张巧艳钱鸣蓉汪建妹吴俐勤肖英平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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