52 results about "Amantadina" patented technology

The osmotic devices of the present invention contain a unitary core comprising a salt of amantadine and an osmotic salt, wherein the two salts have an ion in common. The release rate of the amantadine is modified from a first order release profile to a zero order, pseudo-zero order or sigmoidal release profile by increasing the amount of the osmotic salt in the core of the device. The osmotic device includes a semipermeable membrane having a controlled porosity that can be adapted as needed to cooperate with the osmotic salt in providing a predetermined drug release profile. The osmotic salt need not be coated and it is in admixture with the amantadine salt.

Methods of nighttime administration of amantadine to reduce sleep disturbances in patient undergoing treatment with amantadine are described, as well as compositions of extended release amantadine that are suitable for nighttime administration.

The invention belongs to the technical field of poultry product detection and relates to a combined detection method of amantadine, rimantadine, ribavirin and moroxydine residues in eggs. The method comprises the following steps: carrying out low temperature repetitive freeze-thawing on a sample, adding a formic acid-methanol solution, mixing, centrifuging, taking a supernatant for standby, adding water saturated n-hexane, mixing, carrying out ultrasonic treatment, centrifuging to remove n-hexane floccules on the upper layer with an extracting solution left at the lower layer, purifying with a cation exchange solid-phase extraction column, measuring by a liquid chromatography-tandem mass spectrometer provided with an ESI source, and carrying out accurate qualitative and quantitative analysis on residues of the four antiviral drugs in eggs. The method provided by the invention has high specificity, can accurately and simultaneously measure residual quantity of amantadine, rimantadine, ribavirin and moroxydine without pollution, has high sensitivity, and provides technical support for guaranteeing quality safety of eggs.

The invention discloses an amantadine artificial hapten and an artificial antigen as well as a preparation method and application of the amantadine artificial hapten and the artificial antigen. The molecular structural formula of the amantadine artificial hapten is as shown in the formula (I), and the molecular structural formula of the amantadine artificial antigen is as shown in the formula (II). The application is to utilize the amantadine artificial antigen to prepare an amantadine antibody. The amantadine artificial hapten disclosed by the invention retains the feature structure of amantadine to the largest extent, comprises active groups capable of being linked and coupled with carrier protein as an antigen determinant; the prepared and obtained amantadine artificial antigen can be immunized to obtain the amantadine antibody with high affinity, high sensitivity and high specificity; the titer of immune serum obtained by immunizing a New Zealand white rabbit is up to 1:70000; the amantadine artificial hapten and the artificial antigen can be used for quick and accurate immunodetection and immunoassay to amantadine.

The invention relates to a method, which serially connects ultrahigh performance liquid chromatography (UPLC)-electrospray ionization (ESI) with mass spectrum (MS) to detect the residual quantity of amantadine, rimantadine, and moroxydine in hairs of livestock and poultry.

The invention relates to a N-(3-pyridine methoxy)-3,5-dimethyl-1-amine diadamantane for treatment of Alzheimer's Disease or a salt thereof for medicinal purpose, and also relates to the method for synthesizing the compound or the salt thereof for medicinal purpose, as well as the application in the preparation of drugs for treatment of Alzheimer's Disease, particularly, the compound is a synergetic prodrug produced through chemical coupling of amido link between nicotinic acid and amine diadamantane hydrochloric, on one hand, the prodrug not only avoids the side effects caused by nicotinic acid which is orally taken, but also implements cooperative dual effects on prevention and treatment of Alzheimer's disease by nicotinic acid and amine diadamantane, thereby improving the treatment effect of amine diamantine hydrochloric on Alzheimer's Disease; on the other hand, the enhanced molecular solubility of amine diadamantane in lipid facilitates the passage of the prodrug through a blood-brain barrier, increases the distribution concentration of the prodrug in the cerebrospinal fluid, thereby accomplishing the aim of reducing the peripheral side effects of the drug.

The invention discloses an antigen, an antibody and an enzyme-linked immunosorbent assay (ELISA) kit of amantadine. Firstly, an amantadine hapten is prepared, wherein the molecular structural formulaof the amantadine hapten is as shown in formula (I) (The formula is shown in the description.); then, the amantadine hapten is coupled with carrier protein to obtain an artificial antigen; and then, amonoclonal antibody of amantadine is obtained through a hybridoma technique, thereby establishing a competitive ELISA method and kit according to the monoclonal antibody of amantadine. The ELISA kitof amantadine, disclosed by the invention, can be used for detecting the content of amantadine in an animal tissue sample, the detection limit is 0.10mu g / kg, IC50 is 0.79mu g / L, and the linear detection range is 0-16.2mu g / L; and the method is simple, convenient and quick, is low in cost, high in sensitivity and highstrong in specificity, and is suitable for on-site quick detection.

The invention discloses a compound isatic root injection for animals and preparation technology, which is characterized by the following: allocating main medicine, adjunct and dissolvent; choosing the main medicine from isatic root, linbevelin, moroxydine and amantadine hydrochloride; choosing the adjunct from anti-oxidant, metal chelating agent and dissolvent for injection. This invention possesses advanced productive technology and long shelf life.

The invention discloses a method for detecting the releasing rate of amantadine hydrochloride sustained release tablets. The method comprises the steps of under chromatographic conditions that a high performance liquid chromatograph-evaporative light-scattering detector is adopted and methyl alcohol-acetonitrile-isopropanol-aqueous solution (in a ratio of 50:20:20:10) is taken as a flowing phase, processing six units of amantadine hydrochloride sustained release tablets with 100ml of 0.1mol / L hydrochloric acid solution as a releasing medium at a rotating speed of 75r / min according to a dissolution rate measurement method, taking 5ml of solution after one hour, two hours, four hours and eight hours respectively, filtering the extracted solution while supplementing the solution by 5ml in time, and measuring subsequent filtrate to obtain a test solution; adding water into a contrast to prepare a 0.1mg / ml solution serving as a contrast solution; and recording chromatogram maps of the two solutions, wherein the volume of each solution is 20 microliter. According to the releasing rate detection method, the defect that memantine hydrochloride with a relatively stable structure cannot be effectively detected as being free of absorption in an ultraviolet region is overcome; under a simple flowing phase condition, the dissolving rate of memantine hydrochloride can be accurately detected; the releasing rate detection method has the advantages of simplicity, quickness and accuracy.

The invention discloses an amantadine hapten, a corresponding artificial antigen and a preparation method and application of the amantadine hapten and the corresponding artificial antigen. The amantadine hapten is a product as shown in formula 1, and the amantadine antigen can be obtained by connecting the product with carrier protein. The amantadine antigen can be used for preparing an amantadine specific antibody. The preparation method is simple and practicable, low in cost and high in hapten yield. The amantadine artificial antigen has the advantages that the specific antibody aiming at amantadine can be produced by immunizing animals, the amantadine artificial antigen can be used for preparing an enzyme-linked immunosorbent assay kit for detecting amantadine residues, and the amantadine artificial antigen is simple, fast, large in sample processing amount, high in sensitivity, high in specificity, and the like.

Preparation and application of a highly specific amantadine artificial antigen belong to the technical field of biochemistry engineering. The method is as below: adding amantadine, 6-bromo-hexanoic acid to toluene, heating to 130 DEG C under nitrogen protection, and conducting reflux for 48 h; concentrating under reduced pressure, adding water, and adjusting the pH value of the aqueous phase with hydrochloric acid; adding ethylacetate for extraction, washing with saturated NaCl solution, drying and concentrating to obtain a product semiantigen; and coupling the carboxyl on the semiantigen with amino on a carrier protein to obtain an artificial antigen. Experimental results show that an antiserum obtained by immunizing animals with the antigen obtained by the invention has titer up to 81000, detection limit of 0.1ng / mL, and half inhibitory concentration of 1ng / mL; and the produced antibody has high specificity and high sensitivity. The antigen or antibody provided by the invention has wide application prospects.

The invention provides extended release amantadine compositions for once daily administration of amantadine to a subject.

Methods of administration of amantadine to improve movement disorders are described, as well as compositions suitable therefor.

The present invention discloses a specific monoclonal antibody capable of recognizing amantadine and rimantadine, wherein the specific monoclonal antibody is secreted by a hybridoma cell line 2G3, and the hybridoma cell line 2G3 is preserved in China Center for Type Culture Collection, and has the preservation number of CCTCC NO:C201625. The invention further discloses an enzyme-linked immunosorbent assay method for detecting the amantadine residue and the rimantadine residue in edible animal tissues, and a kit thereof. According to the present invention, the prepared monoclonal antibody has advantages of high sensitivity, high detection accuracy, good precision, gradual change, rapidness, easy operation and the like, and overcomes the gap in the ELISA method on the market.

The invention relates to the field of preparation of haptens, in particular to an amantadine hapten and a preparing method and application thereof. When the amantadine hapten is prepared, amantadine and bromine polyhydroxyalkanoate serve as raw materials, and through a nucleophilic substitution reaction, amidogen groups in amantadine molecules are connected with the bromine polyhydroxyalkanoate; then through a hydrolysis reaction, carboxyl groups are introduced to obtain a corresponding product. The amantadine hapten can be coupled with a carrier protein to prepare an artificial antigen, the artificial antigen is prepared into a monoclonal antibody or a polyclonal antibody through an immune animal, and then the monoclonal antibody or the polyclonal antibody can be used for quickly detecting residues of the amantadine in an animal product.

The invention discloses a test strip for semi-quantitatively detecting amantadine and a preparation method and application of the test strip, and relates to the field of food safety detection. The test strip can be used for simply, quickly and semi-quantitatively detecting amantadine without professional instruments and equipment and professional laboratory auxiliary detection, the use mode is simple and does not need technical training, the result can be directly identified by naked eyes during manual operation and use, and the on-site detection efficiency of amantadine is greatly improved. The result can be conveniently obtained through on-site sampling investigation during market supervision, and detection and result interpretation of a to-be-detected object can be rapidly and on-site realized through the test strip. Meanwhile, the residual content of amantadine can be rapidly and semi-quantitatively judged through four detection lines with different concentration gradients, and the test strip has a wide application prospect in the aspect of rapid immunodetection of amantadine drugs and is suitable for large-scale popularization and application.

The invention discloses a multi-nano transmission system which is obtained by dissolving host molecules and guest molecules in water according to an equal molar ratio, fully stirring for reaction, dialyzing and freeze-drying, wherein the guest molecules are obtained by coupling DSPE-PEG-NHS with amantadine hydrochloride under an alkaline condition. The preparation method of the host molecules comprises the following steps: firstly, taking beta-cyclodextrin as an initial raw material, carrying out ring opening on glycidol under the alkaline condition to obtain beta-cyclodextrin grafted hyperbranched polyglycerol beta-CD-HPG; then taking dicarbonyl imidazole as a coupling reagent, and linking tris (2-aminoethyl) amine to hydroxyl of beta-CD-HPG so as to obtain beta-cyclodextrin grafted hyperbranched polyglycerol grafted tris (2-aminoethyl) amine beta-CD-HPG-TAEA; and then carrying out targeted modification on the beta-CD-HPG-TAEA by using a targeted reagent so as to obtain the host molecules. The multi-nano transmission system can load small molecular drugs and nucleic acid fragments at the same time, and the loaded small molecular drugs and nucleic acid fragments can be flexibly adjusted according to use requirements.

The invention discloses a method for determining the residual quantity of ribavirin and amantadine compounds in poultry food. The method comprises: establishing an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) method to detect the residual quantity of ribavirin and amantadine compounds in poultry food; performing enzymolysis on a sample, trichloroacetic acid precipitating proteins, performing low temperature high speed centrifugation, adjusting the pH value of the supernatant, and purifying through a PBA / PCX composite solid-phase extraction column; analyzing ribavirin by virtue of an Agilent ZORBAX SB-Aq column (3.0 mm * 100mm, 1.8 mu m), analyzing amantadine by virtue of a Waters BEH C18 chromatographic column (2.1 mm * 100mm, 1.7 mu m), measuring by virtue of tandem mass spectrometry, and quantifying by virtue of an isotope internal standard method. The method has the characteristics of simplicity, convenience, quickness, high sensitivity, accuracy in qualitative analysis and the like.

The invention belongs to the technical field of material analysis, and provides a method for detecting amantadine compounds and triazine herbicides in algae. According to the method, acetonitrile, ethyl acetate, a formic acid-acetonitrile mixed solution with the formic acid volume concentration of 1% or a formic acid-ethyl acetate mixed solution with the formic acid volume concentration of 1% are adopted as an extracting agent, so that amantadine compounds and triazine herbicides in an algae sample can be released; a PSA adsorbent is used as an adsorbent; impurities which have great interference on amantadine compounds and triazine herbicides in an extracting solution can be adsorbed and removed to the greatest extent, qualitative detection is performed by adopting ultrahigh performance liquid chromatography, primary mass spectrometry and secondary mass spectrometry, the qualitative accuracy can be improved, false positive can be eliminated, and the detection accuracy can be improved; the amantadine compounds and the triazine herbicides can be accurately quantified by performing quantitative analysis on the amantadine compounds and the triazine herbicides by using ultrahigh liquid chromatography and primary chromatography, and the method has relatively high detection sensitivity.

The invention relates to a medicine combination of amantadine / folic acid compounds and a purpose thereof, and belongs to the technical field of pharmacy. The medicine combination comprises the amantadine with an officinal dose, the folic acid compounds with an officinal dose, and a carrier which can be accepted in pharmacy. The dose of the amantadine is 50-200 mg, and the dose of the folic acid compounds is 0.2-1.6 mg. The medicine combination has the advantages that through synergistic effect of multiple target points, curative effect on the Parkinson's disease is improved, life quality of a patient is improved, through a homocysteine (Hcy) target point, cerebral apoplexy of the patient with the Parkinson's disease can be effectively prevented, occurring risk of the cerebral apoplexy can be effectively lowered, and in addition, the patient can conveniently take medicine.

Disclosed herein are amantadine binding polypeptides, fusion proteins thereof, and uses of such polypeptides and fusion proteins.

The invention discloses an aptamer for specifically recognizing amantadine and application of the aptamer. The nucleotide sequence of the aptamer for specifically recognizing amantadine is shown as SEQ ID NO. 1. According to the aptamer, a systematic evolution technology of library immobilized exponential enrichment ligands is utilized, amantadine is taken as a target, and an amantadine aptamer sequence with high affinity and high specificity is obtained through screening; and the aptamer sequence obtained through screening is high in sensitivity, easy to prepare and modify and can be applied to rapid detection of amantadine veterinary drug residues in food.

The invention discloses an amantadine hapten, an antigen, a chemiluminiscence enzyme-linked immunosorbent assay kit thereof and an application thereof. The invention discloses the amantadine hapten, and also discloses the corresponding artificial antigen and the chemiluminiscence enzyme-linked immunoassay kit and the application thereof. The amantadine hapten provided by the invention is coupled with a carrier protein to obtain an amantadine antigen. The amantadine antigen can be applied to preparation of an amantadine specific antibody. An amantadine chemiluminiscence enzyme-linked immunoassay method is established on the basis of antibody preparation. The detection range is 0 to 16.2 [mu]g / L; the half inhibition concentration is 0.45 [mu]g / L; the detection limit is 0.20 [mu] g / L, the addition recovery rate is 90.00%-104.22%, the whole detection process can be completed only in about 30 min, the detection limit is lower, the sensitivity is higher, and a more convenient, faster and simpler method is provided for detection of residues of amantadine in tissue samples.

The invention discloses an LCMS / MS (Liquid Chromatography Mass Spectrometry / Mass Spectrometry) determination method for the residual quantity of amantadine in eggs. The method comprises the steps of egg sample extraction, purification, standard substance solution preparation, sample determination and the like. A sample pretreatment method which is simple, convenient, rapid and capable of effectively avoiding matrix interference in the sample is established, the pretreatment method is combined with LCMS / MS to be applied to qualitative confirmation and quantitative detection of amantadine in eggs, the average recovery rate is 96.60%-100.19%, the average relative standard deviation (RSD) is 0.06%-0.16% (in-batch) and 0.03%-0.06% (between-batch), the detection limit is 0.3 [mu]g / kg, and the quantification limit is 1 [mu]g / kg. The method has the advantages of simplicity and convenience in operation, quickness, accuracy, high sensitivity and good repeatability.

The invention discloses a continuous production method and device of amantadine, and the method is as follows: uniformly mixed raw materials are continuously fed into a high-viscosity reactor preheated to a certain temperature, the materials rotate along with a stirring shaft in the high-viscosity reactor and move to a discharge port along the stirring shaft, and gas generated in the reaction process is discharged through a secondary sublimation material condensation bin; the first-stage condensed material is automatically cleaned and returned to the reactor, the second-stage condensed material is collected and purified to obtain adamantanol, the reacted material is continuously discharged from a discharge port of the reactor, an amantadine crude product is obtained through collection, and the amantadine crude product is treated to obtain an amantadine product. The method is simple to operate, high in product yield and purity, short in reaction time, high in production efficiency and small in equipment and site occupation, the collected and purified adamantanol can be sold, the mass and heat transfer effect of the reaction is enhanced by adopting the high-viscosity reactor, the safety risk caused by rapid heating and rapid gas production in the intermittent amination reaction process is avoided, and the method is suitable for industrial production. And the method is a continuous industrial production process with high safety.

The invention discloses amantadine organic acid ionic salt as well as a preparation method and the application thereof. The ionic salt comprises anions and cations. The precursors of the anions are organic acids, and precursors of the cations are amantadine. The amantadine organic acid ionic salt provided by the embodiment of the invention not only has pharmacological properties (antiviral and bacteriostatic properties and the like) of amantadine and an organic acid precursor, but also has good water solubility; and damage to organs caused by poor water solubility of adamantane in the oral administration process is avoided.