Combined detection method of amantadine, rimantadine, ribavirin and moroxydine residues in eggs

Abstract

The invention belongs to the technical field of poultry product detection and relates to a combined detection method of amantadine, rimantadine, ribavirin and moroxydine residues in eggs. The method comprises the following steps: carrying out low temperature repetitive freeze-thawing on a sample, adding a formic acid-methanol solution, mixing, centrifuging, taking a supernatant for standby, adding water saturated n-hexane, mixing, carrying out ultrasonic treatment, centrifuging to remove n-hexane floccules on the upper layer with an extracting solution left at the lower layer, purifying with a cation exchange solid-phase extraction column, measuring by a liquid chromatography-tandem mass spectrometer provided with an ESI source, and carrying out accurate qualitative and quantitative analysis on residues of the four antiviral drugs in eggs. The method provided by the invention has high specificity, can accurately and simultaneously measure residual quantity of amantadine, rimantadine, ribavirin and moroxydine without pollution, has high sensitivity, and provides technical support for guaranteeing quality safety of eggs.

Description

technical field [0001] The invention belongs to the technical field of poultry product detection, and relates to a non-polluting, high-efficiency multiplex method for determining amantadine, rimantadine, ribavirin and morpholinidine residues in poultry eggs. Background technique [0002] Since the 1960s, more than 20 antiviral drugs have been developed and marketed by human beings. Among them, four drugs, such as amantadine, rimantadine, ribavirin, and morpholinoguanidine, have a certain inhibitory effect on influenza virus. They are transplanted for veterinary use. Although they have a certain inhibitory effect on viruses such as avian influenza, they lack scientific norms and experimental data on safety and effectiveness. If these drugs are used in large quantities for a long time, it will lead to problems such as animal poisoning, immunosuppression, and drug residues. At the same time, it can also cause the virus to mutate, which will eventually cause great harm to human ...

AI Technical Summary

Problems solved by technology

Both GC and GC-MS require a complex derivatization process before detection, and the operation process is relatively complicated, while LC-MS / MS with high selectivity and high sensitivity has certain advantages in the detection of antiviral drug compounds. However, better pretreatment methods are still needed to reduce the matrix effect of LC-MS / MS, especially for complex animal tissue samples, which have certain limitations

[0004] Chinese patent (CN201210228633.9) discloses an analysis method for the detection of diazepam and its metabolites in human urine samples by LC-MS / MS method, which can only detect a single drug and metabolites, and the pretreatment method is also different from that of The processing of animal tissue samples is very different. Therefore, a sample preparation method that can reduce the matrix effect of LC-MS / MS and an effective method for separating target compounds for qualitative and quantitative determination is urgently needed.

Images