Biocontrol strain for reducing crofton weed allelopathic effect and using method of biocontrol strain for reducing crofton weed allelopathic effect

A technology of biocontrol strains and effects, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve the problems of weakening competitiveness and reducing the allelopathic effect of Eupatorium adenophorum

Inactive Publication Date: 2015-03-11
XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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Problems solved by technology

If it can reduce the allelopathy of Eupatorium adenophorum and weaken its competitive ability, it will provide a new way to effectively control the invasion of Eupatorium adenophorum, but there is no successful report in the prior art

Method used

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  • Biocontrol strain for reducing crofton weed allelopathic effect and using method of biocontrol strain for reducing crofton weed allelopathic effect

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Effect test

Embodiment

[0034] Example Ⅱ, Arthrobacter sp. ZS3 Degradation DDT and HHO Quantitative Determination

[0035] 1. Preparation of standard curves for allelochemicals DDT and HHO

[0036] DDT and HHO standard solutions with concentrations of 5, 20, 80, 120, and 200 μg / mL were prepared with HPLC grade methanol. Each concentration was injected 3 times, the results were averaged, the peak area was plotted against the concentration, and the standard curve was drawn.

[0037] 2. Determination of the ability of ZS3 strain to degrade DDT and HHO

[0038] The strain ZS3 was inserted into 40mL nutrient medium for expansion for 2 days, centrifuged at 4000r / min for 5 minutes, the precipitate was suspended in 1mL sterile water, and the concentration of the bacterial suspension was determined by plate counting method. Take 100uL of the bacterial solution and insert it into 10mL of inorganic salt culture solution containing 200ug of DDT and HHO, culture on a shaking table for 5 days, and take samples t...

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Abstract

The invention discloses a biocontrol strain for reducing the crofton weed allelopathic effect and application of the biocontrol strain for reducing the crofton weed allelopathic effect. The biocontrol strain is arthrobacteria (Athrobacter sp.)ZS3 and preserved in the common microbe microorganism center of the China committee for culture collection of microorganisms; and the preservation number CGMCC NO.9283. The arthrobacterium ZS3 enables herba lycopi diketone and hydroxy herba lycopi ketone with the concentration of 100 ug / mL to be degraded by 49.8% and 56.8% in an inorganic salt culture medium within five days. After fungicides with concentration of 109-1010 CFU / mL are sprayed in the proportion of 4mL / 100g soil, and the crofton weed allelopathic effect is reduced by 46.8%. It shows that the biocontrol strain can effectively degrade allelochemicals, and the biocontrol strain has a wide application prospect on preventing and controlling crofton weeds.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a biocontrol bacterial strain capable of effectively reducing the allelopathic effect of the alien invasion plant Eupatorium adenophorum. At the same time, the present invention also relates to a method for using the biocontrol bacterial strain in biological control. Background technique [0002] Ageratina adenophorum (Ageratina adenophorum) is a vicious perennial invasive alien weed of Compositae. It is native to Mexico and Costa Rica in Central America, and is now widely distributed in more than 30 tropical and subtropical countries and regions in the world. The invasion of Eupatorium adenophorum has caused serious impact on the local ecological environment, mainly in crowding out the growth of other local plants, forming a single superior population, and destroying biodiversity. After Eupatorium adenophorum invades farmland, forest land, and pasture, it c...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02
CPCA01N63/00C12N1/205C12R2001/06
Inventor 李扬苹冯玉龙景兆鹏
Owner XISHUANGBANNA TROPICAL BOTANICAL GARDEN CHINESE ACAD OF SCI
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