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A cell addressing microfluidic chip, cell analysis device and method

A microfluidic chip, addressing technology, applied in the field of medical devices, can solve problems such as stimulation

Active Publication Date: 2017-07-18
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Practice has proved that the three types of methods can effectively achieve drug stimulation of cells, but the existing methods still need to be further improved in terms of solution switching time, cell environment control, and spatial control of stimulation sites.

Method used

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  • A cell addressing microfluidic chip, cell analysis device and method
  • A cell addressing microfluidic chip, cell analysis device and method
  • A cell addressing microfluidic chip, cell analysis device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A microfluidic chip is a polydimethylsiloxane (PDMS) microfluidic chip, which is formed by bonding a polydimethylsiloxane (PDMS) thin layer with a microchannel structure and a glass sheet , with a chip height of 40 μm.

[0046] The surface of the PDMS thin layer is processed with a microchannel structure, such as figure 2 As shown, it includes cell culture channel 1, a pair of side pressure channels 2, addressing channel 3, stimulation channel 4 and waste liquid channel 5.

[0047] The culture channel is used to cultivate adherent cells and observe the state of the cells, and its inlet port is connected to the lateral pressure channel 2, the addressing channel 3 and the stimulation channel 4; the lateral pressure channel 2 is symmetrically arranged on both sides of the culture channel, and its outlet The addressing channel 3 and the stimulating channel 4 are symmetrically arranged, and the outlet end is connected to the inner side of the inlet end of the culture chann...

Embodiment 2

[0052] A microfluidic chip is a polydimethylsiloxane (PDMS) microfluidic chip, which is formed by bonding a polydimethylsiloxane (PDMS) thin layer with a microchannel structure and a glass sheet , with a chip height of 40 μm.

[0053] The surface of the PDMS thin layer is processed with a microchannel structure, such as image 3 As shown, it includes cell culture channel 1, a pair of side pressure channels 2, addressing channel 3, stimulation channel 4 and waste liquid channel 5.

[0054] The culture channel is used to cultivate adherent cells and observe the state of the cells, and its inlet port is connected to the lateral pressure channel 2, the addressing channel 3 and the stimulation channel 4; the lateral pressure channel 2 is symmetrically arranged on both sides of the culture channel, and its outlet The addressing channel 3 and the stimulating channel 4 are symmetrically arranged, and the outlet end is connected to the inner side of the inlet end of the culture channe...

Embodiment 3

[0059] A microfluidic chip is a polydimethylsiloxane (PDMS) microfluidic chip, which is formed by bonding a polydimethylsiloxane (PDMS) thin layer with a microchannel structure and a glass sheet , with a chip height of 40 μm.

[0060] The surface of the PDMS thin layer is processed with a microchannel structure, such as Figure 4 As shown, it includes cell culture channel 1, a pair of side pressure channels 2, addressing channel 3, stimulation channel 4 and waste liquid channel 5.

[0061] The culture channel is used to cultivate adherent cells and observe the state of the cells, and its inlet port is connected to the lateral pressure channel 2, the addressing channel 3 and the stimulation channel 4; the lateral pressure channel 2 is symmetrically arranged on both sides of the culture channel, and its outlet The addressing channel 3 and the stimulating channel 4 are symmetrically arranged, and the outlet end is connected to the inner side of the inlet end of the culture chann...

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Abstract

The invention discloses a cell addressing micro-fluidic chip, a cell analysis device and a cell analysis method. The chip includes a cell culture channel, a pair of side pressure channels, an addressing channel, a stimulation channel and waste liquid channels. During work, when the chip is in an addressing state, the pressure of the addressing channel is greater than that of the stimulation channel; when the chip is in a stimulated state, the pressure of the stimulation channel is greater than that of the addressing channel. The cell analysis device comprises the micro-fluidic chip, a signal acquisition device and electric proportioning conversion valves, wherein the inlet ends of the side pressure channels, the addressing channel and the stimulation channel of the chip are respectively connected with the independent electric proportioning conversion valves; each electric proportioning conversion valve outputs corresponding hydraulic pressure according to different electrical signals; the signal acquisition device is arranged in the cell culture channel and used for collecting cell signals or imaging cells. According to the invention, the technical problems such as solution switching time, cellular environment control and stimulating point space control in an existing micro-fluidic based cell stimulation method are solved.

Description

technical field [0001] The invention belongs to the field of medical equipment, and more specifically relates to a microfluidic chip, a cell analysis device and a method. Background technique [0002] Cells are the basic unit of all known life in nature except viruses, and the basis of all life activities. Therefore, in-depth research on cells is the key to uncovering the mysteries of life and transforming life. The ability of cells to recognize and respond to their microenvironment through signal transduction is crucial for the development, tissue repair, and immunity of organisms. Cell stimulation is one of the most effective ways to study intracellular signal transduction, intercellular communication, and high-throughput drug screening. When cells are stimulated by different chemical drugs, they will trigger the spatiotemporal changes of cell response, regulate the electrophysiological activity of cells, thereby affecting the growth and differentiation of cells and maint...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/34C12Q1/02
CPCC12M23/16C12M33/00C12M35/04
Inventor 冯晓均陈鹏刘笔锋郭怡然李一伟
Owner HUAZHONG UNIV OF SCI & TECH
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