Method for preparing special nutrient liquid preparation for hatching penaeus vannamei

Abstract

The invention relates to a special nutrient liquid preparation for hatching penaeus vannamei and a preparation method of the special nutrient liquid preparation. The liquid preparation comprises bacillus pumilus of which the preservation number is CCTCC M 2013240, magnesium, zinc, copper, cobalt, vitamin A, vitamin D, vitamin E, vitamin B1, vitamin B2, vitamin C, stomach protease and tryptase. The invention further provides the preparation method of the special nutrient liquid preparation. The special nutrient liquid preparation has the advantages that the nutrients are comprehensive and balanced, the survival rate and the growth speed of penaeus vannamei are increased, and the disease is reduced. The preparation method of the special nutrient liquid preparation provided by the invention is simple in process and high in raw material utilization rate.

Description

technical field

[0001] The invention relates to the technical field of feed, in particular to a preparation method of a special nutrient liquid preparation for hatching of Penaeus vannamei. Background technique

[0002] Vannamei Boone (Litopenaeus Vannamei Boone, 1931), also known as Litopenaeus Vannamei Boone, also known as Litopenaeus Vannamei Boone, etc., its shape is similar to that of Chinese prawn and Penaeus meji, with a maximum body length of 23cm and a carapace. Thin, the normal body color is blue, without markings all over the body; the legs are chalky, so it is called white-legged shrimp or white-legged shrimp. The shrimp is native to the waters of the Pacific coast of Central and South America, and the distribution along the coast of Ecuador is the most concentrated. In the early 1970s, Ecuador took the lead in trial breeding and succeeded in one fell swoop. Later, many shrimp farming countries or regions introduced it one after another, and the vannamei aquacu...

Examples

Embodiment 1

[0017] The bacillus pumilus bacterial strain is cultivated in the order of slant, liquid seed, and liquid fermentation, and the slant and seed medium are conventional nutrient agar medium or broth medium; fermentation medium formula (W / V): bean cake powder 3% , 1.5% starch, 1% corn steep liquor, 0.5% glucose, (NH 4 ) 2 SO 4 0.1%, NH 4 Cl0.1%, KH 2 PO 4 0.05%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, the fermentation culture condition is 28-37 ℃, ventilated culture 36-48h, the number of viable bacteria ≥ 2×10 9 cfu / ml.

[0018] Add mineral elements, vitamins and enzymes to the concentrated solution of Bacillus pumilus, the amount added per 1000ml concentrated solution of Bacillus pumilus: MgCl 2 ·6H 2 O101g, zinc sulfate 27g, copper methionine 19g, cobalt methionine 36g, 500,000 IU / g feed grade vitamin A1.2g, 500,000 IU / g feed grade vitamin D 3 6g, vitamin E17.5g, vitamin B 1 6.5g, vitamin B 2 5g, vitamin C30g, pepsin 65g, tryptase 85g.

Embodiment 2

[0020] The broodstock of Penaeus vannamei is cultivated by conventional methods, and the unilateral eye stalk is removed. After the female shrimp matures, the male shrimp with healthy limbs is carefully selected to mate with the female shrimp. The specific method is to transfer mature female shrimps to an aquarium of 0.60m×0.60m×0.60m, and put two female shrimps and two male shrimps in each box. box. Females that did not mate were returned to their original aquariums. After the spawning is over, the female shrimp is removed from the spawning box to avoid loss of eggs. The egg-laying box is divided into a control group and an experimental group. The egg-laying box of the control group is a conventional egg-laying box, and the egg-laying box of the experimental group has added the special nutrient liquid preparation for hatching of Penaeus vannamei in Example 1, and the addition amount is 100% for hatching. Protective liquid preparation: the volume ratio of water body is 1:100...

technical field

[0001] The invention relates to the technical field of feed, in particular to a preparation method of a special nutrient liquid preparation for hatching of Penaeus vannamei. Background technique

[0002] Vannamei Boone (Litopenaeus Vannamei Boone, 1931), also known as Litopenaeus Vannamei Boone, also known as Litopenaeus Vannamei Boone, etc., its shape is similar to that of Chinese prawn and Penaeus meji, with a maximum body length of 23cm and a carapace. Thin, the normal body color is blue, without markings all over the body; the legs are chalky, so it is called white-legged shrimp or white-legged shrimp. The shrimp is native to the waters of the Pacific coast of Central and South America, and the distribution along the coast of Ecuador is the most concentrated. In the early 1970s, Ecuador took the lead in trial breeding and succeeded in one fell swoop. Later, many shrimp farming countries or regions introduced it one after another, and the vannamei aquacu...

Examples

Embodiment 1

[0017] The bacillus pumilus bacterial strain is cultivated in the order of slant, liquid seed, and liquid fermentation, and the slant and seed medium are conventional nutrient agar medium or broth medium; fermentation medium formula (W / V): bean cake powder 3% , 1.5% starch, 1% corn steep liquor, 0.5% glucose, (NH 4 ) 2 SO 4 0.1%, NH 4 Cl0.1%, KH 2 PO 4 0.05%, NaH 2 PO 4 0.5%, MgSO 4 0.05%, MnSO 4 0.05%, the fermentation culture condition is 28-37 ℃, ventilated culture 36-48h, the number of viable bacteria ≥ 2×10 9 cfu / ml.

[0018] Add mineral elements, vitamins and enzymes to the concentrated solution of Bacillus pumilus, the amount added per 1000ml concentrated solution of Bacillus pumilus: MgCl 2 ·6H 2 O101g, zinc sulfate 27g, copper methionine 19g, cobalt methionine 36g, 500,000 IU / g feed grade vitamin A1.2g, 500,000 IU / g feed grade vitamin D 3 6g, vitamin E17.5g, vitamin B 1 6.5g, vitamin B 2 5g, vitamin C30g, pepsin 65g, tryptase 85g.

Embodiment 2

[0020] The broodstock of Penaeus vannamei is cultivated by conventional methods, and the unilateral eye stalk is removed. After the female shrimp matures, the male shrimp with healthy limbs is carefully selected to mate with the female shrimp. The specific method is to transfer mature female shrimps to an aquarium of 0.60m×0.60m×0.60m, and put two female shrimps and two male shrimps in each box. box. Females that did not mate were returned to their original aquariums. After the spawning is over, the female shrimp is removed from the spawning box to avoid loss of eggs. The egg-laying box is divided into a control group and an experimental group. The egg-laying box of the control group is a conventional egg-laying box, and the egg-laying box of the experimental group has added the special nutrient liquid preparation for hatching of Penaeus vannamei in Example 1, and the addition amount is 100% for hatching. Protective liquid preparation: the volume ratio of water body is 1:100...

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