Supercharge Your Innovation With Domain-Expert AI Agents!

Polypeptide-magnetic nanoparticles of circulating tumor cells with high expression of her2 and their preparation method and application

A magnetic nanoparticle and tumor cell technology, applied in the field of polypeptide-magnetic nanoparticle and its preparation, can solve problems affecting the health and life of patients, poor prognosis, etc., and achieve good reproducible results

Active Publication Date: 2016-11-23
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that breast cancer (20-35%) and ovarian cancer (8-11%), two major malignant tumor cells, highly express HER2 protein, and the prognosis is poor, seriously affecting the health and life of patients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polypeptide-magnetic nanoparticles of circulating tumor cells with high expression of her2 and their preparation method and application
  • Polypeptide-magnetic nanoparticles of circulating tumor cells with high expression of her2 and their preparation method and application
  • Polypeptide-magnetic nanoparticles of circulating tumor cells with high expression of her2 and their preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1. Verification of polypeptide specific recognition of HER2

[0068] The polypeptide fragments used (synthesized by Shanghai Keyept Biotechnology Co., Ltd., with a purity of 98%) are shown in Table 1 below; magnetic nanoparticles: streptavidin-magnetic nanoparticles (Ademtech, 03121, France).

[0069] Table 1

[0070]

[0071]

[0072] SK-BR-3 cells were used as HER2-positive cells, and the combination rate of anti-HER2 and SK-BR-3 cells was used as a positive control. Flow cytometry was used to detect the three types of anti-HER2 and MCF-7, A549 and K562. cell binding rate. Based on this, the cell line with the lowest binding rate to anti-HER2 was searched for as the HER2-negative cell line.

[0073] The results of flow cytometry experiments were as follows: figure 1 As shown, the results showed that the binding rates of anti-HER2 to SK-BR-3, A549, MCF-7 and K562 were BR Anti / SK-BR-3 =83.87%, BR Anti / A549 = 16.44%, BR Anti / MCF-7 =13.91%, BR Anti / ...

Embodiment 2

[0074] Example 2 The flow cytometry method detects that the polypeptides have different effects on human HER2 positive and negative breast cancer cells. cell binding

[0075] The binding effect of the polypeptide of the present invention on HER2-positive cell lines and K562 cells is detected by flow cytometry method, and the specific method comprises the following steps:

[0076] (1) A series of FITC (fluorescein isothiocyanate)-peptide-biotin (Biotin) was designed and synthesized, and HER2-positive SK-BR-3 and HER2-negative K5620 cells were used as test objects. Flow cytometry and HER2-positive SK-BR-3 and HER2-negative K562 cells, GG40, QN37 and GG19 designed by the present invention can specifically bind to HER2-positive cells, but for HER2-negative cells, GG40 and QN37 show certain Negative binding properties, low selectivity; while the binding rate of GG19 to HER2 negative cell K562 is very low, high selectivity.

[0077] The detection process includes the following st...

Embodiment 3

[0102] Comparison of binding rates between polypeptides and HER2-positive breast cancer cells at different concentrations in Example 3

[0103] Taking polypeptide QN37 as an example, different concentrations of polypeptide QN37 are combined with HER2-positive breast cancer cells. The method specifically includes the following steps:

[0104] 1) The effect of the amount of designed peptide on the binding rate of designed peptide to SK-BR-3 cells

[0105] The concentration of antibody anti-HER2 is C anti-HER2 =52 μg / mL, the molecular weight is 150 kDa, the number of cells in the flow cytometry experiment is 106, and the antibody dosage is 0.01733 μM. The molecular weight of the antibody is relatively large, tens to a hundred times that of the designed polypeptide. Compared with antibodies, polypeptides have fewer amino acids, smaller molecular weights, smaller volumes, and greater spatial freedom. Therefore, when polypeptides interact with the cell surface, they are likely t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a polypeptide of human epidermal growth factor receptor 2 (HER2) high-expression circulating tumor cells, and in particular relates to a polypeptide-magnetic nanoparticle for enriching, separating and detecting circulating tumor cells (CTC) as well as a preparation method and an application of the polypeptide-magnetic nanoparticle. The amino acid sequence of the polypeptide is shown in one of SEQ ID NO: 1-18; the polypeptide-magnetic nanoparticle contains magnetic nanoparticle and the polypeptide coupled with the magnetic nanoparticle and used for specifically recognizing the tumor cells; and the polypeptide or the polypeptide-magnetic nanoparticle can be widely used for preparing products for enriching, separating and detecting circulating tumor cells or for preparing medicines for treating metastasis-associated diseases. The invention provides a novel method for the specific recognition of cell surface antigen by virtue of the polypeptide and for rapidly and accurately detecting the CTC; therefore, an effective method is provided for the diagnosis, treatment and prognosis of metastasis patients clinically.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, in particular to a polypeptide, in particular to a circulating tumor cell ( Circulating Tumor Cell, CTC) polypeptide-magnetic nanoparticles and its preparation method and application. Background technique [0002] Cancer is one of the major diseases that threaten human beings. Invasion and metastasis are one of the most prominent features of malignant tumors. Tumor cells detach from the primary tumor spontaneously or due to diagnostic and therapeutic operations, and undergo Epithelial-Mesenchymal Transition (EMT) , so that it has flow characteristics and enters the peripheral blood to form circulating tumor cells (CTC). Therefore, the detection of circulating tumor cells (CTCs) in peripheral blood indicates the possibility of tumor metastasis. In addition, the latest research from Harvard Medical School in the United States shows that in situ tumor tissue can attract CTC cells to re-...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/54326G01N33/574G01N33/6893
Inventor 王琛赵琼彭佳茜杨延莲白林灵
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com