Bioactive hyaluronic acid syrup preparation
A hyaluronic acid and biologically active technology, applied in the direction of non-central analgesics, anti-inflammatory agents, drug combinations, etc., can solve problems such as poor effect and no effective treatment methods, so as to relieve discomfort symptoms and increase human body treatment Effect, effect of increasing biological activity
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Embodiment 1
[0029] Objective: Production method of bioactive hyaluronic acid fragments.
[0030] Method: The raw material is 200-600KD macromolecular hyaluronic acid with a purity of 90.0%-99.9%, which is cut with recombinant human hyaluronidase PH20 produced by CHO cells, and 1000-2000 units of hyaluronidase is used per gram of hyaluronic acid. Digest at 37 degrees for 4-6 hours, pH5.0-5.5, 130-160mM NaCl, 0.5-1.0mMMgSO 4 , 1.5-2.5mM CaCl 2 , the final molecular weight is 0.7KD-100KD hyaluronic acid fragments.
[0031] At least one end of the hyaluronic acid fragment with a molecular weight of 0.7KD-100KD is the end cleaved by recombinant human hyaluronidase PH20 produced by CHO cells that does not cause allergic reactions in humans, has glycation, and can recognize the normal structure of hyaluronic acid. It has the biological activity of promoting cell secretion of defensin 2, killing microorganisms and inhibiting skin and mucous membrane inflammation. The biological activity depends...
Embodiment 2
[0033] Objective: To study the effect of different syrup formulation excipients on the human biological activity of bioactive hyaluronic acid.
[0034] Method: In this experiment, the method of human experiment was used to detect the biological activity of bioactive hyaluronic acid in human body, that is, the effect of different high-concentration ointment and ointment accessories on the effect of bioactive hyaluronic acid on the treatment of laryngeal and pharyngeal inflammation.
[0035] The pre-experiment on small animals in the early stage of this experiment has found that xanthan gum, modified guar gum, carrageenan, pectin, gelatin, vegetarian meat powder, agar, starch (including collagen, soybean protein hydrolyzate) , yeast protein hydrolyzate) all reduce or do not increase the human biological activity of biologically active hyaluronic acid.
[0036]All cases in this experiment were patients with pharyngitis. Experimental group 1 (sorbitol) took sorbitol 40% + bioactiv...
Embodiment 3
[0049] Bioactive Hyaluronic Acid Low Sugar Syrup
[0050] Formula (percentage by weight): 10% bioactive hyaluronic acid, 40% sorbitol, 1% white sugar, 2% brown sugar, 6.7% maltitol, 0.01% mint flavor, 1% honey, 0.3% sodium benzoate, the balance for water. Namely the formula of Experimental Group 1 of Example 1.
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