Method for extracting snapdragon from water chestnut peel

A technology of snapdragon and water chestnut skin, applied in the direction of organic chemistry and the like, can solve unseen and other problems, and achieve the effects of low production cost, good separation effect, and scientific and reasonable design.

Active Publication Date: 2017-01-18
贺州市顺来农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In our research, we found that water chestnut skin is rich in aureusidin, but there is no literature report on the extraction and isolation of this compound from water chestnut skin

Method used

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  • Method for extracting snapdragon from water chestnut peel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In the extraction tank, add 800g of fresh water chestnut skin powder and 6.4L of 50% by volume acetone aqueous solution, extract at 50°C for 2 hours, extract 3 times in total, filter, combine the filtrates, concentrate under reduced pressure into a paste, and obtain 120g of extract .

[0022] Add 900 mL of water to the extract to make a turbid liquid, add 1.8 L of ethyl acetate to extract three times, combine the extracts, concentrate under reduced pressure, and obtain 10.5 g of the extract.

[0023] Dissolve the extract in 25mL of methanol, then add 250mL of water to make a suspension, transfer it to a 1kg D101 macroporous resin chromatography column for adsorption, after static adsorption, wash with water until the effluent is colorless, and then use 25% volume percentage 800mL of methanol aqueous solution to wash away the polar glycoside impurities, and finally eluted with 50% methanol aqueous solution, detected by thin-layer chromatography, collected and combined th...

Embodiment 2

[0026] In the extraction tank, add 800g of fresh chestnut skin powder and 1.6L of 75% volume percent methanol aqueous solution, extract at 70°C for 2.5h, filter, and extract 3 times in total, combine the filtrates, concentrate under reduced pressure to obtain 150g of extract .

[0027] Add 750 mL of water to the extract to make a turbid liquid, then add 1.5 L of ethyl acetate to extract 3 times, combine the extracts, concentrate under reduced pressure, and obtain 12.0 g of the extract.

[0028] Dissolve the extract in 24mL of methanol, then add 240mL of water to make a suspension, transfer it to a 1kg S-8 macroporous resin chromatography column for adsorption, after static adsorption, wash with water until the effluent is colorless, and then use 25 800mL of aqueous methanol solution of % volume percentage washes away the polar glycoside impurities, and finally elutes with 50% aqueous methanol solution by volume percentage, detects by thin-layer chromatography, collects the ant...

Embodiment 3

[0031] In the extraction tank, add 800g of fresh water chestnut skin powder and 8.0L of 70% ethanol aqueous solution by volume, extract at 65°C for 1.5h, extract 2 times in total, filter, combine the filtrates, concentrate under reduced pressure into a paste, and obtain the extract 100g.

[0032] Add 800 mL of water to the extract to make a turbid liquid, add 1.6 L of ethyl acetate to extract three times, combine the extracts, concentrate under reduced pressure, and obtain 9.5 g of the extract.

[0033] Dissolve the extract in 19mL of methanol, add 190mL of water to make a suspension, transfer it to a chromatographic column of 1kg D101 macroporous resin for adsorption, after static adsorption, wash with water until the effluent is colorless, and then use 25% volume percentage 800mL of ethanol aqueous solution washes away the polar glycoside impurities, and finally elutes with 45% ethanol aqueous solution by volume percentage, detects by thin-layer chromatography, collects and ...

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Abstract

The invention discloses a method for extracting aureusidin from eleocharis tuberosa peels, and belongs to the field of natural organic chemistry. The method is characterized by comprising the following steps: taking any one of methanol aqueous solution, ethanol aqueous solution and acetone aqueous solution as the solvent, extracting aureusidin from eleocharis tuberosa peels, separating the extract with macroporous adsorption resin after extraction with ethyl acetate, and purifying the obtained crude products with a gel chromatography column to obtain aureusidin products with the purity of above 95%. According to the method for extracting aureusidin from eleocharis tuberosa peels, a large number of waste eleocharis tuberosa peels are used as the raw materials for preparing aureusidin, so that the waste eleocharis tuberosa peels are recycled, and a new approach to extract and separate aureusidin from natural plants is provided; the separation effect is good; the purity of products is high; the selected macroporous resin and gel can be used repeatedly; the method has the advantages of accessible raw materials, abundant resources and low production cost, can perform large-scale production, and can meet requirements of the pharmaceutical industry.

Description

technical field [0001] The invention belongs to the field of natural organic chemistry, and relates to a method for extracting and separating aureusidin, in particular to a method for extracting and separating aureusidin from water chestnut skin. Background technique [0002] Aureusidin, chemically named 4,6,3′,4′-tetrahydroxyaurone, is the most famous aurone compound, which has various biological activities such as antioxidant, antibacterial, and antitumor. Antirrhinum has a special structure, and it is rarely distributed in plants in nature, and its content in plants is low, so it is difficult to obtain on a large scale. In order to facilitate the study of its pharmacological activity, scholars usually use synthetic methods to obtain aureusidin. Hu Kun from Changzhou University reported the total synthesis method of aureusidin, which uses 2,4,6-trihydroxyacetophenone and 3,4-dihydroxybenzaldehyde as raw materials to synthesize aureusidin through five-step reactions. The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D307/83
CPCC07D307/83
Inventor 罗杨合何星存李行任谢玉花黄永兰
Owner 贺州市顺来农业发展有限公司
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