Detection kit for early diagnosis of colorectal cancer and application of detection kit

A detection kit and early diagnosis technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of low specificity, poor sensitivity, and non-disclosure, and achieve high detection efficiency and strong pertinence Effect

Inactive Publication Date: 2015-04-29
上海汇真生物科技有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fiberoptic colonoscopy, the gold standard for early diagnosis of colorectal cancer, is an invasive examination that requires complete bowel preparation, privacy exposure and inconveniences such as sedation, and is difficult to be widely accepted by people
Fecal occult blood test is currently the standard non-invasive method for early screening of colorectal cancer recommended clinically, ...

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  • Detection kit for early diagnosis of colorectal cancer and application of detection kit
  • Detection kit for early diagnosis of colorectal cancer and application of detection kit
  • Detection kit for early diagnosis of colorectal cancer and application of detection kit

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Embodiment Construction

[0031] A detection kit for early diagnosis of colorectal cancer, the detection kit includes three pairs of rRNA_pseudogene, HECTD1 and ZNF843 gene promoter region methylation-specific amplification primers and three methylation Specific sequencing primers:

[0032] Nucleotide sequences of rRNA_pseudogene methylation-specific upstream primers:

[0033] 5'-TGGGATTTTAGATTTTTTTTGAAGG-3'

[0034] Nucleotide sequences of rRNA_pseudogene methylation-specific downstream primers:

[0035] 5'-AACCTAAAAACCTCAAATTATTTTAAATCA-3'

[0036] rRNA_pseudogene methylation-specific sequencing primers:

[0037] 5'-GGATTTTAGATTTTTTTTGAAGGTA-3'

[0038] Nucleotide sequences of HECTD1 methylation-specific upstream primers:

[0039] 5'-GATTGGAGGATAGTTTTGGGTTTTTA-3'

[0040] Nucleotide sequences of HECTD1 methylation-specific downstream primers:

[0041] 5'-AACTTACTACTACTTCTCCAAAACTTC-3″

[0042] HECTD1 methylation-specific sequencing primers:

[0043] 5'-GTTGTGGTTTTTTAGTATTAAATTT-3'

[0044] ...

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Abstract

The invention discloses a detection kit for early diagnosis of colorectal cancer. The detection kit comprises three pairs of specific amplification primers which are respectively used for detecting rRNA-pseudogene, HECTD1 and ZNF843 gene promoter and endogene region methylation and a methylation-specific sequencing primer, wherein the three pairs of specific amplification primers which are respectively used for detecting rRNA-pseudogene, HECTD1 and ZNF843 gene promoter and endogene region methylation comprise methylation-specific forward primers and methylation-specific reverse primers. According to the detection kit, auxiliary diagnosis of the colorectal cancer is realized by detecting the methylation degree of the rRNA-pseudogene, HECTD1 and ZNF843 gene promoter region and endogene region associated with the colorectal cancer; the detection kit is simple, convenient, high in detection efficiency and high in targeting property, has the advantages of accuracy, reliability, flexibility, rapidness and economy and contributes to early discovery and timely treatment of the colorectal cancer.

Description

technical field [0001] The present invention relates to the technical field of early diagnosis of colorectal cancer, in particular to a detection kit for early diagnosis of colorectal cancer and its application, especially to a detection of rRNA_pseudogene, HECTD1 and ZNF843 genes related to colorectal cancer A kit for the degree of methylation in a promoter region and its application. Background technique [0002] Due to changes in living habits, the incidence of colorectal cancer in our country has been increasing year by year, and it has ranked the top 3 to 4 in the incidence of all tumors in men and women. Surgical treatment can cure early colorectal cancer, while the average survival time of advanced colorectal cancer after chemoradiotherapy combined with molecular targeted therapy is still less than 30 months. Therefore, the early diagnosis of colorectal cancer is very important and urgent. Fiberoptic colonoscopy, the gold standard for early diagnosis of colorectal c...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/154
Inventor 王红卫王琳刘元华
Owner 上海汇真生物科技有限公司
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