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Multiplex suspension assay/array using lifetime coding

A composite array, lifetime technology, applied in the field of composite analysis and/or arrays, can solve problems such as hindering the decoding process

Active Publication Date: 2015-05-20
MACQUARIE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although in principle an imaging-based decoding system can decode any suspension array, small variations in the height of some particles in the plane will seriously hinder the intensity-based decoding process

Method used

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  • Multiplex suspension assay/array using lifetime coding
  • Multiplex suspension assay/array using lifetime coding
  • Multiplex suspension assay/array using lifetime coding

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Embodiment Construction

[0044] The following manner of description given by the examples is intended to provide a more precise understanding of the subject matter of the preferred embodiment.

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Abstract

A system, device and / or method for multiplex assays. In a particular, but non-limiting, example there is provided a multiplex array, such as a suspension array. Luminescence decay lifetimes are utilised for probes in a suspension array, and coding / decoding the codes from time-resolved spectra. Lifetime populations can be generated at distinct colour bands. A novel temporal technique or dimension is applied over conventional spectral and intensity combinations, thereby expanding the multiplexing capacity of a suspension array. In one example form, the multiplexing capacity of a suspension array can be expanded to the order of about 58. This provides a reliable, high-throughput and relatively inexpensive solution for multiplex assays in various areas of application such as life sciences, data storage and security.

Description

Technical field [0001] The present invention relates generally to composite analysis and / or arrays; in one example, it more specifically relates to suspension-based analysis and / or arrays. In other words, the present invention provides a method, system and / or device used in the field of biotechnology and life science examples, such as the determination and quantification of biomolecular species and anti-counterfeiting such as anti-counterfeiting ink or printing. Background technique [0002] The frontiers of molecular biology require high-throughput analysis techniques that can simultaneously identify and quantify a large number of biomolecular species. This covers a wide range of bioinformatics fields including genomics, proteomics, intercellular and molecular signaling, metabolomics, cytoomics, and personalized medicine. For example, because everyone's genetic characteristics vary greatly, and traditional symptom diagnosis techniques are often unable to diagnose, the emerging ...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N21/64C09K11/77B82Y15/00G01N33/543C40B70/00C12M1/34
CPCG01N21/6408B42D25/382B42D25/387C09D11/50C09K11/02C09K11/025C09K11/06C09K11/7773C09K2211/182C40B70/00G01N21/6428G01N33/542G01N33/582
Inventor 金大勇陆怡青赵江波
Owner MACQUARIE UNIV
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