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Method for restoring eutrophic tidal flat sediment habitat

A technology of eutrophication and sedimentation, applied in the field of habitat restoration, can solve the problems of poor permeability of polymer substrates, limited diffusion of carriers, low mechanical strength, etc., achieve good environmental protection value and market application prospects, and improve the environment The effect of the parameter

Active Publication Date: 2015-06-03
INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it also has some disadvantages, such as the carrier has a limited diffusion effect, and many carriers have poor permeability to polymer substrates after forming a gel, and the mechanical strength is often low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of bacterial solution: The bacterial strain is a denitrifying bacterial strain, which is obtained by separating and screening from marine tidal flat sediments. Add the bacterial solution to the enriched culture solution. At a temperature of 30°C, anaerobic culture was carried out for 72 hours, and the bacteria reached the exponential growth phase, and the bacterial liquid was collected for fixation.

[0033] (2) Configuration of fixative: mix sodium alginate with 60ml seawater according to the mass of 4g, 1g, 1g, 1g, 1g respectively, add starch, bentonite, zeolite powder and shell sand immediately after heating and dissolving 20 mesh sieve), fully stirred and mixed, and cooled to room temperature to obtain about 70ml of fixative.

[0034] (3) Mixing and gelation of the bacterial solution and the fixative solution: mix 80ml of the bacterial solution that has grown to reach the growth index phase with the prepared 70ml fixative solution, and stir on a mag...

Embodiment 2

[0042] (1) Preparation of bacterial solution: The bacterial strain is a denitrifying bacterial strain, which is obtained by separating and screening from marine tidal flat sediments. Add the bacterial solution to the enriched culture solution. At a temperature of 30°C, anaerobic culture was carried out for 72 hours, and the bacteria reached the exponential growth phase, and the bacterial liquid was collected for fixation.

[0043] (2) Configuration of fixative: mix sodium alginate with 70ml of seawater according to the mass of 4g, 1g, 1g, 1g, 1g respectively, add starch, zeolite powder, shell sand and activated carbon immediately after heating and dissolving, fully stir and mix, Cool to room temperature to obtain a fixative.

[0044] (3) Mixing and gelation of the bacterial solution and the fixative solution: mix 80ml of the bacterial solution that has grown to reach the growth index phase with the prepared 70ml fixative solution, and stir on a magnetic stirrer at a speed of ...

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Abstract

The invention relates to a method for restoring a eutrophic tidal flat sediment habitat. The method comprises the following steps of: preparing bacteria solution; preparing stationary liquid; uniformly mixing and gelling the bacteria solution and the stationary liquid; preparing CaC12 solution; fixedly granulating the gelled bacteria solution; collecting and preserving; carrying out combined operation of plowing and environmental restoration bacteria immobilized particles; arranging shellfishes and clam worms; and regularly monitoring. The method disclosed by the invention is capable of effectively protecting bacterial strains and has the buffer function in an environment that physical and chemical factors are more severe; an effective vector can be provided for the bacterial strains, so that the bacterial strains have the bacteria restoration function effectively for a long time; by means of bioturbation of the shellfishes and the clam worms, the nitration and denitrification effects of sediments can also be effectively increased; and in addition, by means of combined use of the above methods, the method has good environment-friendly value and market application prospect to environmental restoration.

Description

technical field [0001] The invention belongs to the field of habitat restoration, in particular to a method for habitat restoration of eutrophic tidal flat sediments. Background technique [0002] Immobilized biotechnology is a new technology that has developed rapidly since the 1960s. It uses physical or chemical means to locate free cells or enzymes in a limited space and keep them active and A basic technology that can be used over and over again. Immobilized microorganism technology has the advantages of high microbial density, rapid reaction, less microbial loss, easy separation of products, and easy control of the reaction process. Common methods include adsorption, embedding, covalent bonding, and cross-linking. Among them, embedding is the most studied and widely used. [0003] The embedding and immobilization method is to embed microbial cells in the tiny spaces of the gel or in the ultrafiltration membrane of the semipermeable membrane polymer ([6] Qu Yang, Zhang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/02C12N11/14C12N11/10C12N11/04C02F103/08
Inventor 沈辉万夕和孙瑞建缪甜黎慧王李宝凌云乔毅史文军戚原野
Owner INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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