Use of molecular marker detection object in preparation of kit for detecting liver cancer

A technology of molecular markers and detection substances, applied in the field of biomedicine, can solve the problems of low accuracy and achieve the effect of improving accuracy

Inactive Publication Date: 2015-06-03
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI +1
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the defect that the accuracy rate is still relatively low when miRNA is used as a molecular marker to detect the pathological stage and poor prognosis of liver cancer, and to provide a pathological stage that can detect liver cancer with a high accuracy rate and poor prognosis

Method used

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  • Use of molecular marker detection object in preparation of kit for detecting liver cancer
  • Use of molecular marker detection object in preparation of kit for detecting liver cancer
  • Use of molecular marker detection object in preparation of kit for detecting liver cancer

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preparation Embodiment 1

[0022] Under the ethical and legal standards, 130 autologous liver cancer liver tissue samples and adjacent normal tissue samples were collected, and these samples were stored in liquid nitrogen for later use. The basic clinical conditions of these samples are shown in Table 1.

[0023] Table 1

[0024] age

Embodiment 1

[0026] Use the miRNA extraction kit (product number DP501) purchased from Tiangen Biochemical Technology Co., Ltd. to extract miRNA from 130 cases of autologous liver cancer liver tissue samples and paracancerous normal tissue samples in Preparation Example 1 to obtain their respective RNA sample. Then, the miRNA cDNA first-strand synthesis kit (product number KR201) was used to perform tailing reverse transcription on the above RNA samples to obtain miRNA cDNA samples. Then use the miRNA fluorescence quantitative detection kit (product number FP401) and the primers shown in Table 2 to carry out tailing method qRT-PCR amplification on the above-mentioned miRNA cDNA sample, carry out qRT-PCR quantitative detection of RNU6 and GAPDH, and the detection results as shown in Table 3.

[0027] Table 2

[0028]

[0029] table 3

[0030]

[0031]In Table 3, the low expression of miR-19a means that the expression of miR-19a in the liver tissue samples of liver cancer is reduced...

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Abstract

The invention provides use of a molecular marker detection object in preparation of a kit for detecting liver cancer. The molecular marker detection object comprises a detection object which can specifically detect miR-19a and a detection object which can specifically detect cell cycle proteins D1(Cyclin D1). According to the use of the molecular marker detection object, the accuracy rate of detection of histopathologic stage and poor prognosis of the liver cancer can be improved.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the use of a molecular marker detection substance in the preparation of a kit for detecting liver cancer. Background technique [0002] Primary hepatocellular carcinoma (hereinafter referred to as liver cancer) is a common tumor with a high mortality rate. The incidence of liver cancer ranks sixth among all tumors, but ranks third in global cancer mortality, of which about 55% occur in our country. Due to the insidious onset of liver cancer, the clinical symptoms are extremely atypical. About 70% of small liver cancers no larger than 5cm are asymptomatic, so liver cancer is often difficult to be detected early. When patients have obvious clinical symptoms, they are often in the late stage of liver cancer. Most patients Optimal healing time has been lost. The occurrence of liver cancer is the result of the comprehensive action of many factors, involving the disorder or mutation of man...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6886C12Q2600/112C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 张彦琼郭晓冬赵景民林娜杨美
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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