Immunoaffinity stir bar for adsorbing raxofelast acid lactone compound, and preparation method and application thereof

Abstract

The invention discloses an immunoaffinity stir bar for adsorbing raxofelast acid lactone compound, and a preparation method and an application thereof. The immunoaffinity stir bar for adsorbing raxofelast acid lactone compound is composed of solid phase carrier and zeranol murine monoclonal antibody coupled with the same, wherein the ratio of the coupling length of the solid phase carrier and coupled zeranol murine monoclonal antibody to the weight thereof is 1 cm: 2 mg. Through combining with a chromatography, an extracting method can effectively separate and condense the ingredients to be detected in a sample, the raxofelast acid lactone compound detection is facilitated, the disadvantages of less information amount, low quantification precision, low physico-chemical method selectivity and the like due to using the simplex immunoassay to directly detect the sample are overcome, and the analysis mechanism complementarity between the immunological technique and conventional physical and chemical technology is reflected.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and relates to an immunoaffinity stirring rod and a preparation method thereof. In particular, it relates to an immunoaffinity stirring bar for absorbing racolactone compounds, its preparation method and application. Background technique [0002] With the development of my country's economy and the improvement of people's living standards, the demand for various animal-derived foods is gradually increasing, and food safety issues have naturally become the focus of everyone's attention. In recent years, there have been many reports about the harm to human health caused by veterinary drug residues, and relevant food safety departments are also committed to the research of detection methods. Due to the complex components of biological samples, the easy combination of drugs and sample components, and the low concentration of residual drugs, it has brought great difficulties to the extr...

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Problems solved by technology

The TLC method is low in cost, but the sensitivity and selectivity are not high; the ELISA method is highly sensitive, but due to its cross-reaction characteristics, the selectivity is poor; the GC-MS method has good sensitivity and selectivity, but requires derivatization, operation It is tedious and time-consuming; HPLC method can be directly determined, but for complex matrix samples, impurities interfere greatly, and there are certain difficulties in qualitative confirmation; LC-MS / MS method does not need to achieve complete chromatographic separation of the analyte and interfering components. It can confirm the structure of the compound with higher sensitivity and accuracy, but it is expensive and often limited in practical applications

Biological sample pretreatment technologies mainly include liquid-liquid extraction and solid-phase extraction. Liquid-liquid extraction is cumbersome and requires a large amount of organic solvents. Solid-phase extraction has the advantages of less organic solvents, strong selectivity, and short separation time. At present, C18 and HLB solid-phase extraction cartridges are commonly used for the determination of zearalanol. Although common solid-phase extraction cartridges are convenient and fast to operate, they have poor specificity.

The Immunoaffinity SPME technology was first proposed in 2001, and the use of Immunoaffinity stir bar to purify rexolactone compounds has not yet been reported, and there is no commercialized Immunoaffinity stir bar for sale

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