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Molecular marker s5.7 co-segregated with spinach sex gene y and its application

A technology of molecular markers and co-segregation, applied in the field of plant molecular genetics and breeding, can solve the problem of insufficient stability of V20A amplification effect, and achieve the effect of improving breeding efficiency

Inactive Publication Date: 2017-01-18
中蔬种业科技(北京)有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are 4 published markers linked to the spinach sex gene Y at home and abroad. The first one is the co-segregation of T11A, V20A and the spinach sex gene Y, but the amplification effect of V20A is not stable enough in the verification of large groups, and the co-segregation of T11A and Y ; The second is SO4, which is 4.3cM away from the spinach sex gene Y, and it is not linked when verified in the population. On this gene fragment, another SSR is developed for analysis, and the genetic distance is about 11cM; the third is Yang Jinhua (2009 ) by ISSR analysis, screened a female-associated marker with a full length of 1176bp, and converted it into a SCAR marker that was not linked when validated in the population

Method used

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  • Molecular marker s5.7 co-segregated with spinach sex gene y and its application
  • Molecular marker s5.7 co-segregated with spinach sex gene y and its application
  • Molecular marker s5.7 co-segregated with spinach sex gene y and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Acquisition of Molecular Marker S5.7 Co-segregated with Spinach Sex Gene Y

[0037] 1. Construction of spinach genetic population

[0038] The F2 population was constructed by using female plants×male plants of the first-generation commercial spinach hybrid variety Donglu 1 (F1). The traits of the F2 population were investigated at the flowering stage ( figure 1 , figure 2 ), there were 343 plants in the F2 population, including 189 female plants and 154 male plants, which were 1:1 by chi-square test, and the sex of spinach was controlled by sex gene X / Y.

[0039] 2. Genomic DNA extraction and construction of male and female pools

[0040] Genomic DNA of 343 F2 individual plants was extracted by CTAB method.

[0041] Take 10 female plants and 10 male plants in F2 to construct female and male pools for Bulk Segregating Analysis (BSA), and measure the concentration of DNA with a DNA detector (NanoDrop Spectrophotometer). Dilute to the same concentration of...

Embodiment 2

[0051] Example 2 Application of Molecular Marker S5.7

[0052] Using the successfully transformed SCAR marker S5.7, construct F2 populations in the other 7 populations of spinach [Description of populations: Female plants × male plants in the first-generation spinach hybrid commercial variety Spinach (F1), and the female plants in the F2 population × Male plants were used to construct F3 populations, and 4 F3 populations were selected for molecular marker gender identification (that is, the experimental population numbers were 48, 50, 52, and 53 respectively); F2 population was constructed by plant x male plant, and F3 population was constructed by female plant x male plant in F2 population, and three F3 populations were selected for molecular marker sex identification (that is, the experimental population numbers were 54, 58, 59)]. Gender identification, molecular marker identification results are consistent with phenotype identification results, see image 3 , so it can be ...

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Abstract

The invention provides a molecular marker S5.7 co-separated from a spinach gender gene Y and application of the molecular marker S5.7 and belongs to the field of molecular genetic breeding of plants. The molecular marker S5.7 can amplify special bands of 126bp in male spinach plants and does not amplify bands in female spinach plants, and the primer sequence of amplification is as shown in SEQ ID NO. 2-3. The molecular marker S5.7 is co-separated from the spinach gender gene and can identify the spinach gender in the seedling stage, so that the female and male spinach plants can be screened in advance, and the breeding efficiency is greatly improved. The molecular marker S5.7 has an important value in the spinach production practice and breeding.

Description

technical field [0001] The invention belongs to the field of plant molecular genetics and breeding, and in particular relates to molecular marker S5.7 co-separated with spinach sex gene Y and application thereof. Background technique [0002] Spinach (Spinacia oleracea L.), also known as Persian grass and red root vegetable, belongs to the genus Spinacia, has a long history of cultivation and is one of the important vegetable crops in my country. Spinach is rich in nutrition, strong cold resistance, wide adaptability, short production cycle, high multiple cropping index, high yield and output value, and is very popular among producers and consumers. [0003] Spinach is generally a dioecious crop, and a small number of plants are monoecious. Spinach sex type is affected by genetic factors and environmental factors. Rosa et al. (1925) believed that the sex type of spinach is mainly controlled by genetic factors, and is not affected by external factors such as soil fertility,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 徐兆生钱伟刘丹张合龙王晓武武剑刘伟
Owner 中蔬种业科技(北京)有限公司