Method for analyzing serum metabolomics on basis of LC-MS (liquid chromatogram-mass spectrograph) serum metabolomics technology

Abstract

The invention discloses a method for analyzing serum metabolomics on the basis of an LC-MS (liquid chromatogram-mass spectrograph) serum metabolomics technology. According to the method, serum is analyzed by constructing a serum metabolomics analysis model, and a model construction method comprises the following steps: collecting a healthy serum sample and an infected serum sample; performing LC-MS detection on the samples to obtain an original metabolic fingerprint; and preprocessing the fingerprint, sequentially performing PCA (principal component analysis) and PLS-DA (partial least squares-discriminant analysis) on an obtained two-dimensional matrix to obtain a PLS-DA model, verifying the obtained model, and if the model does not have over-fitting risks, finishing the model construction. By adopting the method disclosed by the invention, a serum metabolomics analytic technology is applied to early screening of esophageal cancer for the first time, high risk populations of the esophageal cancer can be quickly and conveniently screened out, the screening range of the esophageal cancer is reduced, the efficiency of screening the whole population of a high incidence area of the esophageal cancer is effectively improved, the screening cost is greatly reduced, and the pain caused by invasive endoscopy to part of the population is effectively avoided, so that the method has important economic and social benefits and is convenient to popularize and apply.

Description

technical field [0001] The invention relates to a method for analyzing serum metabolomics based on LC-MS serum metabolomics technology. The method can conveniently and quickly carry out early preliminary screening for esophageal cancer, and belongs to the field of analysis technology. Background technique [0002] Esophageal cancer is a malignant lesion formed by abnormal proliferation of esophageal squamous epithelium or glandular epithelium, and it is a gradual evolution process from atypical hyperplasia to carcinoma. According to the latest data from the World Health Organization, about 400,000 people die from esophageal cancer every year in the world. my country is the country with the highest incidence and mortality rate of esophageal cancer, and 90% of the patients have squamous cell carcinoma. The Wenhe River Basin in Shandong Province is a high-incidence area for esophageal cancer, with Feicheng City, Ningyang County, and Wenshang County having the highest incidence ...

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies in the prior art, such as cumbersome early screening methods for esophageal cancer, high cost, and causing pain to the testing population, there is no convenient, fast, and suitable for a wide range of defects. The present invention provides a serum metabolome based on LC-MS. This method is simple to operate and can replace the iodine staining indicative biopsy technique under the gastroscope for early and preliminary screening of esophageal cancer in the population. Painful, easy to promote and apply

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