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A kind of method for rapidly preparing water-soluble pigment by utilizing Protissae

A technology for water-soluble pigments and prolifera, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve problems such as difficult and effective extraction, and achieve the advantages of less free copper content, simple processing and operation, and improved copperization rate. Effect

Active Publication Date: 2016-10-05
青岛海济润生生物科技有限公司
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AI Technical Summary

Problems solved by technology

[0012] Pheophytin a is yellow-green, which is the main reason why Enteromorpha turns yellow-green, making it difficult to correctly determine and identify Enteromorpha chlorophyll by conventional methods
At the same time, it is difficult to effectively extract the chlorophyll and its degradation products in Enteromorpha using traditional techniques, and a special preparation method is required.
The present invention provides a kind of rapid preparation method of Enteromorpha water-soluble pigment for solving above problem, and this method has not been reported in existing patent and literature

Method used

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  • A kind of method for rapidly preparing water-soluble pigment by utilizing Protissae

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Embodiment 1

[0029] Present embodiment is example with the fresh enteromorpha of 1kg water content 70% to produce water-soluble pigment:

[0030] 1. Weigh 1kg of Enteromorpha raw material, add 2L of water, stir and wash, let it settle for precipitation, remove sand and other impurities, filter to retain the filter residue, add 9g of EDTA-Cu solid powder and 3g of Na 2 S 2 o 3 For solid powder, add water until the filter residue can be completely submerged, boil for 15 minutes, at this time the Enteromorpha changes from yellow-green to dark green. Filter to get the filter residue, wash the filter residue with 5L of clear water, and press filter. Dry the filter residue until the water content is less than 5%, and pulverize it to obtain about 150 g of powder.

[0031] 2. Extract the above-mentioned powder with 1.5 L of acetone, stir and extract at 50° C. for 2 hours, cool down to room temperature, and filter to obtain Enteromorpha pigment extract.

[0032] 3. Add 300 ml of 5% NaOH-ethanol...

Embodiment 2

[0036] In this embodiment, water-soluble chlorophyll is prepared from Enteromorpha powder with a water content of 1kg less than 7% as an example:

[0037] 1. Weigh 1kg of Enteromorpha powder, add 100ml of 30% EDTA-Cu aqueous solution and 10% Na 2 S 2 o 3 100ml, add water until the filter residue can be completely submerged, boil for 15 minutes, at this time the Enteromorpha changes from yellow-green to dark green. Filter to get the filter residue, wash the filter residue with 5L of water, press filter to obtain 800g of Enteromorpha powder.

[0038] 2. Extract the above-mentioned powder with 8L of acetone, stir and extract at 50°C for 2 hours, cool down to room temperature, and filter to obtain Enteromorpha pigment extract.

[0039] 3. Add 1.6L of 5% NaOH-ethanol solution (accounting for about 1 / 5 of the volume of the extract) to the above extract, stir and react at 50°C for 15 minutes to obtain a dark green precipitate, filter the precipitate, and obtain Enteromorpha chloro...

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Abstract

The invention relates to a method for quickly preparing a water-soluble pigment by using enteromorpha. The method mainly comprises the following steps: performing pretreatment on raw material enteromorpha in a water phase; cleaning and performing filter pressing to obtain pretreated enteromorpha powder; performing acetone extraction and alkaline reaction to obtain an enteromorpha chlorophyll water-soluble pigment precipitant; removing impurities out of reaction supernatant by petroleum ether and performing reduced-pressure distillation to obtain an enteromorpha chlorophyll degradable product water-soluble pigment; mixing the pigment precipitants which are obtained in the two steps to obtain an enteromorpha water-soluble pigment finished product. Compared with the traditional chlorophyll water-soluble pigment preparation process, the method provided by the invention aims at instability and high degradation rate of enteromorpha chlorophyll, has the characteristics of easiness for operation, good economical performance, environment friendliness, high efficiency and quickness.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for rapidly preparing water-soluble pigments by utilizing Enteromorpha chlorophyll and degradation products thereof. Background technique [0002] The natural pigments extracted from green plants are mainly chlorophyll and its derivatives, among which fat-soluble pigments are used as green colorants, mainly used in cakes, jams, jellies, surimi products, dried noodles, etc.; and water-soluble pigment products are mainly chlorophyll Copper sodium salt, which is the only green pigment allowed in my country's food industry, is widely used as a colorant in cosmetics, food and medicine. There are many raw materials for the production of chlorophyll, and silkworm excrement was first used. In recent years, some people have experimented with bamboo leaves, reeds, banana leaves, beet leaves, spinach leaves, etc. as raw materials for the production of chlorophyll. [0003] At present, th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D487/22C09B61/00
CPCC07D487/22C09B61/00
Inventor 王鹏黄志俊皎皎
Owner 青岛海济润生生物科技有限公司
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