22 results about "Chlorophyll degradation" patented technology

The invention discloses a transcription factor ZmbHLH180 gene as well as an encoded protein and application thereof, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the corn ZmbHLH180 gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2. The invention provides a transcription factor ZmbHLH180 gene, the CDS full length of the gene is 864bp, 287 amino acids are encoded, the gene is mainly positioned in a cell nucleus, and the expression of a chlorophyll degradation gene NYC1 and the senescence of leaves can be obviously promoted by over-expressing the ZmbHLH180 gene in arabidopsis thaliana; the trans-ZmbHLH180 gene overexpressed corn can remarkably promote leaf greening under drought stress, a certain technical foundation is laid for regulation and control of the plant growth cycle, a choice is provided for genetic improvement of new plant varieties, and the application value is important.

The invention provides a strain of saccharomyces cerevisiae and application of the saccharomyces cerevisiae. The preservation number of the Filobasidium magnum is CGMCC (China General Microbiological Culture Collection Center) No.34231, and the Filobasidium magnum secretes lipoxygenase, protease and amylase. Compared with a control group fermented by sterile water, the saccharomyces cerevisiae fermentation group has the advantages that when the saccharomyces cerevisiae fermentation group is applied to the enhanced fermentation of the tobacco leaves, the contents of carotenoid degradation products, cembrane degradation products, Maillard reaction products, chlorophyll degradation products, terpenoid substances and the like of the tobacco leaves can be remarkably increased, the potassium-chlorine ratio can be increased by 50.1%, and the yield of the tobacco leaves can be increased by 50.1%. The total content of aroma components of the tobacco leaves is increased by 37.7%. According to the invention, the aroma amount, aroma richness, maturity, sweetness and combustibility of cigar tobacco leaves can be obviously improved, irritation and offensive odor are reduced, the aroma characteristics of sweet aroma, costustoot, bean aroma and the like are highlighted, and the fermentation period is shortened to 15 days.

The invention provides a sweet potato leaf powder processing technology with a high nutrition retention rate, which comprises the following steps: S1, putting sweet potato leaves into a dynamic fixation roller, and mixing nitrogen and carbon dioxide by adopting a nitrogen and carbon dioxide mixing generator to form nitrogen and carbon dioxide mixed gas, the nitrogen and carbon dioxide mixed gas is heated to 95 DEG C through a heat exchanger and sprayed out, the oxygen concentration in the dynamic fixation roller is maintained to be smaller than or equal to 0.5%, and the chlorophyll degradation rate of the sweet potato leaves is monitored through a near-infrared sensor by inhibiting the activity of polyphenol oxidase of the sweet potato leaves; according to the method, a weak acid environment is formed through CO2 dissolution to inhibit the activity of polyphenol oxidase and reduce the requirement for fixation temperature, active ingredients are reserved through three-area gradient variable-temperature drying and rapid dehydration and freeze-drying sublimation, energy consumption is reduced by 37%, heat stability is improved through polyphenol glycosylation, the bioavailability of beta-carotene is improved to 80% through nano embedding, and therefore nutrient loss is avoided, and the method is suitable for industrial production. The drying efficiency, color and flavor are improved.

The present application provides a kind of transcription factor PpeNAC33 for regulating plant growth and development and chlorophyll degradation, nucleotide sequence is as shown in SEQ.ID.NO.2, amino acid sequence is as shown in SEQ.ID.NO.3.The present application also provides the vector based on the above-mentioned transcription factor, engineering strain.Meanwhile, the application of the above-mentioned transcription factor PpeNAC33 in regulating plant growth and development and chlorophyll degradation is provided.The present application finds a NAC transcriptional regulatory factor PpeNAC33 in the transcriptome analysis of seed abortion peach fruit.Studies have shown that the transcription factor is a key transcription factor for regulating plant growth and development and chlorophyll degradation, and its expression can be accurately regulated by space-time and expression amount, which can be used to realize the accurate control of plant growth process and avoid the occurrence of plant fruit premature aging.

The invention discloses a postharvest fruit and vegetable fresh-keeping agent as well as a preparation method and application thereof. The fresh-keeping agent comprises the following effective components: ergothioneine with the concentration of 20 ppm to 60 ppm and / or taurine with the concentration of 10 ppm to 40 ppm. The preservative disclosed by the invention is beneficial to retarding of fruit chlorosis, chlorophyll degradation, delaying of fruit softening and aging and the like in the storage and transportation processes of picked fruits and vegetables, is beneficial to prolonging of the shelf life of the picked fruits and vegetables, and has a great potential application prospect. After being treated by the preservative, picked fruits and vegetables can be stored at normal temperature without using large low-temperature equipment or cold-chain transportation, so that the preservative is simple and convenient to operate and easy to popularize.

The application discloses use of PpSec61gamma in regulating chlorophyll degradation in plants. The application discloses that PpSec61gamma has the function of regulating chlorophyll degradation in plants, and when the expression level of PpSec61gamma gene in plants is increased, the color of plant organs becomes yellow, the content of chlorophyll is significantly reduced, and the expression level of genes related to chlorophyll degradation is significantly increased. The PpSec61gamma of the application can be used as a target for regulating the chlorophyll degradation pathway or the organ color trait of plants, and is applied to plant breeding, and therefore has important scientific significance and application value.

The invention discloses an application of an RsERF109a gene in regulation and control of yellowing and aging of radish sprouting vegetable leaves, and is characterized in that a nucleotide sequence of the RsERF109a gene is shown as SEQ ID NO.1, an amino acid sequence of protein encoded by the RsERF109a gene is shown as SEQ ID NO.2, aging of the radish sprouting vegetable leaves is delayed by inhibiting expression of the RsERF109a gene, and the application in plant breeding comprises construction of overexpressed RsERF109a radish sprouting vegetable. The invention also provides an application of the RsERF109a gene in preparation of a chlorophyll degradation related gene expression promoter. The RsERF109a gene has the advantages that the key function of the radish AP2 / ERF family transcription factor RsERF109a in regulating and controlling postharvest yellowing and senescence of the radish sprouting vegetable is identified and verified for the first time, postharvest yellowing of cotyledons of the radish sprouting vegetable can be remarkably accelerated by overexpressing the RsERF109a gene in the radish sprouting vegetable, and the postharvest fruit and vegetable quality can be accurately regulated and controlled through a molecular means.

The invention provides sporidiobolus roseus, application of the sporidiobolus roseus in tobacco leaf fermentation and a biological preparation. The sporidiobolus roseus is preserved in China General Microbiological Culture Collection Center (CGMCC) on April 16, 2025, the address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.34236. The sporidiobolus roseus is named as sporidiobolus roseus, and the sporidiobolus roseus is named as sporidiobolus roseus. The strain can significantly increase the content of carotenoid degradation products, Maillard reaction products, chlorophyll degradation products, terpenoids and the like of tobacco leaves; the aroma amount, the aroma richness and the maturity of the cigar tobacco leaves are improved, and the aroma characteristic of the costustoot is highlighted; meanwhile, the sweetness of the tobacco product can be improved, and the caramel sweet and honey sweet note characteristics are highlighted; and the contents of nicotine, dehydroneonicotine and cotinine can be respectively reduced, so that the irritation and cleanliness of tobacco products are improved.

The invention discloses an application of a broccoli BoCyt f gene in regulating and controlling plant senescence, and is characterized in that the nucleotide sequence of the BoCyt f gene is shown as SEQ ID NO.1. The function of BoCyt f is verified in a transgenic overexpression mode, and overexpression of the BoCyt f gene in arabidopsis thaliana can significantly promote leaf yellowing and senescence and accelerate chlorophyll degradation, so that the Broccoli BoCyt f gene can be used for regulating and controlling plant senescence. Active oxygen accumulation is induced, and PCD related gene expression is up-regulated; the invention has the advantages that the forward regulation effect of the BoCyt f gene in plant PCD and aging is defined for the first time, and a new target gene is provided for plant anti-aging breeding and postharvest preservation technology.

This invention discloses a "vaccine-type" bacteriocin, Yi-VD14, with the amino acid sequence VDFGPHGLLPIRPI, which can induce plant immune responses and enhance plant disease resistance. Taking chili peppers as an example, this bacteriocin significantly improves the storage quality of chili peppers by delaying vitamin C decomposition and chlorophyll degradation and reducing malondialdehyde accumulation. Furthermore, it induces plant immune responses and enhances plant disease resistance by regulating fruit fat metabolism, inhibiting cell membrane degradation, and improving the biosynthesis of plant disease-resistant metabolites. It also increases the activity of disease-resistant enzymes in chili pepper fruits and reshapes the microbial community structure on the surface of the fruits. Ultimately, it enhances the plant's resistance to pathogenic microorganisms from multiple dimensions, reducing the incidence of chili pepper soft rot and minimizing post-harvest losses. This bacteriocin can be used not only to prepare products for controlling chili pepper soft rot but also for controlling other fruit and vegetable diseases, showing promising application prospects in the fruit and vegetable planting and post-harvest distribution industry chain.

The application belongs to the technical field of crop planting, and discloses application of N-cis feruloyl tyramine in improving alkali stress tolerance of rice. ‑8 ~10 ‑4 g / L of the working solution, and spraying the leaves at the one-leaf-one-heart stage of the rice seedlings. The application can significantly improve the chlorophyll content of the rice under alkali stress environment, alleviate chlorophyll degradation, promote the growth of the aboveground part, and effectively enhance the alkali stress tolerance of the rice at the seedling stage through simple foliar spraying operation. The method is simple in operation, low in cost, high in environmental protection and efficiency, and has important agricultural application value for stable yield and yield increase of rice in saline-alkali land and utilization of marginal land resources.

The invention discloses an exogenous potassium comprehensive regulation and control method for promoting yellowing and relieving ash hanging, and relates to the technical field of tobacco leaf cultivation. The method comprises the following steps: 65-75 days after flue-cured tobacco plants are transplanted, performing interface optimization treatment on tobacco leaves, and then preparing and spraying low-strength compound potassium spraying liquid; 75-90 days after flue-cured tobacco plants are transplanted, mechanical induction is conducted on leaves, and then moderate-strength compound potassium spraying liquid is prepared and sprayed; 90-105 days after flue-cured tobacco plants are transplanted, if it is observed that tobacco leaves have the abnormal yellowing symptom in the field, deviation correction regulation and control are carried out, and the deviation correction regulation and control comprise preparing and spraying high-strength compound potassium spraying liquid. According to the invention, chlorophyll degradation and material conversion are effectively promoted, and synchronous and uniform yellowing is realized; and meanwhile, by optimizing the physiological state, the ratio of ash-coated tobacco leaves of the flue-cured tobacco leaves is remarkably reduced, finally, the ratio of first-class tobacco leaves of the flue-cured tobacco leaves is increased, the average price and the grade index are remarkably improved, and both the excellent yield and the stable yield of the tobacco leaves are considered.

The present application belongs to the technical field of cigar processing, and particularly relates to a method for adding old liquor to cigar tobacco to improve flavor and quality of fermentation. The method comprises the following steps: adding unsterilized old liquor in multiple times through moisture recovery, and adopting a three-stage fermentation mode of aerobic-anaerobic-aerobic to obtain cigar tobacco with obviously improved quality. The present application creatively combines Fujian Yongan unsterilized old liquor with cigar for fermentation, adopts multiple spraying of unsterilized old liquor, and adopts a three-stage fermentation mode of aerobic-anaerobic-aerobic, so that the content of phenylalanine conversion products, cembrane degradation products, carotenoid degradation products and chlorophyll degradation products in the cigar tobacco is significantly increased, the content of TSNAs is reduced, the content of reducing sugar and potassium is increased, and the content of nicotine, total nitrogen and chlorine is reduced. The present application optimizes fermentation temperature, humidity and time, so that the content of aroma components such as alcohols, aldehydes and ketones, esters, alkanes and aromatic compounds in the cigar tobacco reaches the maximum value.

The invention provides a strain of Yarrowia guilliermondii and application thereof. The preservation number of the Meyerozyma guilliermondii is CGMCC (China General Microbiological Culture Collection Center) No. 34234, and the preservation number of the Meyerozyma guilliermondii is CGMCC No. 34234. The strain can secrete protease and lipoxygenase, and compared with a control group fermented by sterile water, the Yarrowia guilliermondii fermentation group can significantly increase the content of carotenoid degradation products, Maillard reaction products, chlorophyll degradation products, terpenoids and the like of tobacco leaves by applying the Yarrowia guilliermondii to enhanced fermentation of the tobacco leaves. Meanwhile, the aroma amount, aroma richness, maturity, sweetness and combustibility of the cigar tobacco leaves are remarkably improved, irritation and offensive odor are reduced, and the aroma characteristics of resin aroma, costustoot, nut aroma and the like are highlighted.

The invention provides a composite fresh-keeping method for asparagus, which comprises the following steps: pre-cooling a low-pressure fumigation cabin, putting the asparagus into the low-pressure fumigation cabin, then adjusting the pressure to 20KPa, putting a calcium chloride aqueous solution into an atomization kettle, injecting ozone into the atomization kettle, pressurizing to 20KPa to obtain an atomized substance containing ozone-calcium chloride, injecting the atomized substance containing ozone-calcium chloride into the low-pressure fumigation cabin in which the asparagus is placed, and carrying out fresh-keeping treatment on the asparagus. And carrying out low-pressure combined ozone-calcium chloride synergistic fumigation treatment at 10 DEG C and 20 KPa, packaging, and storing, so as to obtain the asparagus subjected to composite preservation. The method can delay chlorophyll degradation of asparagus, delay lignification of tender stems, inhibit spindling of the tender stems and delay the reduction rate of sensory and nutritional quality of the asparagus, is good in effect, can be commercially applied and popularized on a large scale, and has important economic and social values.

The invention relates to the field of food processing color protection, in particular to a color protection composition and a color protection method for preparing quick-frozen dandelion dishes, the composition comprises sodium ascorbate, calcium lactate and EDTA-2Na, dandelions are washed, then placed in blanching liquid containing the color protection composition to be blanched, quickly fished out after blanching is finished, and subjected to shock cooling with ice water to 10 DEG C or below, and a finished product is obtained. Draining off water, immediately quickly freezing, and freezing and preserving in a refrigerator at the temperature of-18 DEG C; according to the compound color protection formula provided by the invention, the safety risk of heavy metals is avoided, enzymatic browning can be inhibited, and the color of dandelion is stable; the blanching temperature and time parameters matched with the characteristics of the dandelion leaves are optimized, the problems of high-temperature long-time softening and rotting and low-temperature short-time enzyme inactivation are solved, and chlorophyll degradation and nutrition loss are reduced while the activities of polyphenol oxidase and peroxidase are ensured to be rapidly passivated; the synergistic effect of composite color protection and blanching processes is achieved, the process is simplified, and the method is suitable for large-scale production of quick-frozen vegetables.

The invention relates to the technical field of plant preservation, and discloses a preparation method of a multi-component preservation solution for leaf-cut plants, the method takes a general basic formula and an exclusive adapter bag as a core, and the general basic formula contains sucrose, potassium nitrate, magnesium sulfate, 8-hydroxyquinoline citrate and other components and provides energy, nutrition and basic antibacterial guarantee; the special adaptive bags are divided into fiddlehead, woody broad-leaf and herbaceous fine-leaf types, and the characteristics of corresponding cut leaves are adapted through synergistic bacteriostasis and water retention, energy supplementation, cell wall protection and water locking to delay chlorophyll degradation respectively. The preparation method comprises the steps of component weighing, basic liquid preparation, functional component adding, adaptive adjustment, operation protection and waste liquid treatment, parameters such as the stirring rotating speed and time are determined, and it is ensured that the components are evenly dissolved. Precise preservation of the bracken leaves, the woody broad leaves and the herbal thin leaves is achieved, the fresh green period and the shelf life are prolonged, loss is reduced, industrial production and practical application are both considered, and operation is convenient and environmentally friendly.

The invention provides coral hairy sporidium, a fungicide, a preparation method and application, and belongs to the technical field of microbial fermentation and tobacco processing. The preservation number of the coral coremiforme SC-11 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 34235, and the preservation number of the coral coremiforme SC-11 is CGMCC No. 34235. The provided strain can increase the content of carotenoid degradation products, Maillard reaction products, chlorophyll degradation products, terpenoids and the like, and increase the total content of aroma components of tobacco leaves; nicotine and derivatives (nornicotine, dehydroneonicotine and the like) of nicotine can be degraded, the content of alkaloid substances can be effectively reduced, and smoke irritation is reduced; the metabolite can reconstruct the aroma characteristics of cigar tobacco leaves, highlight the multiple aroma levels of honey sweet aroma, resin aroma, flower aroma, coffee aroma and the like, and remarkably improve the soft feeling and fineness of smoke at the same time.

This invention relates to a type of Bacillus safranin (Sarfus). Bacillus safensis HMC19, with accession number CGMCC No. 35493, can stably colonize in high saline-alkali environments, regulate soil pH, and secrete the plant growth regulator phenylacetic acid, which delays chlorophyll degradation and effectively alleviates premature aging of plants under saline-alkali stress. It achieves synergistic effects of saline-alkali land improvement and crop quality and yield enhancement. It can also effectively degrade soil autotoxic substances such as p-hydroxybenzoic acid and ferulic acid, improve the soil microenvironment, and prevent continuous cropping obstacles.

The invention discloses a grinding device for measuring chlorophyll a, which relates to the technical field of water quality monitoring equipment and comprises a base, a control unit, a sealed grinding component, a low-temperature control component, a reagent quantitative adding component and a sample collecting component. The sealing grinding assembly adopts the sealing design of a light-proof grinding cavity and a sealing cover and is matched with a scraping plate to scrape residues on the inner wall, a grinding head is matched with the grinding cavity and is provided with a grinding bulge, and gradual efficient grinding of a sample is realized; the low-temperature control assembly maintains a low-temperature environment through a spiral flow channel of the cooling jacket, and chlorophyll degradation is inhibited; the reagent quantitative adding assembly realizes automatic quantitative adding of acetone; the sample collection assembly is sealed and conveyed through a piston, and sample loss is reduced. The device is high in automation degree, capable of reducing acetone volatilization and improving the chlorophyll a extraction rate, extremely low in sample residual rate, suitable for sample pretreatment of chlorophyll a determination in water areas such as oceans and lakes, capable of improving determination efficiency and accuracy and capable of guaranteeing health of operators.

The invention discloses a baking method for promoting chlorophyll degradation of reviving tobacco leaves, and relates to the field of tobacco leaf baking. The method comprises a high-temperature and high-humidity yellowing promoting step, a low-temperature and low-humidity slow color fixing step and a stable-temperature, humidity-discharging and stem-drying step. In the middle stage of the yellowing stage, the activity of hydrolase is activated and maintained by maintaining a high-temperature and high-humidity environment with the dry-bulb temperature being 38 DEG C and the wet-bulb temperature being 36 DEG C, smooth starting of chlorophyll degradation is promoted, and in the end stage of the yellowing stage, a temperature stabilizing point with the dry-bulb temperature being 40 DEG C and the wet-bulb temperature being 36 DEG C is additionally arranged, and chlorophyll degradation of the branch veins is promoted; temperature stabilizing points with the dry-bulb temperature being 44 DEG C and the wet-bulb temperature being 36 DEG C are additionally arranged at the initial stage of the color fixing stage, and the difficulty that thick and strong main veins turn yellow is emphatically overcome. According to the method, a temperature and humidity control curve from the yellowing stage to the initial color fixing stage is systematically optimized, a key temperature stabilizing point is introduced, a gradient slow temperature rising strategy is adopted, and the technical problem that dehydration and yellowing of the reviving tobacco leaves are difficult to coexist is solved.

The invention belongs to the technical field of bamboo wood processing and drying, and particularly relates to a color-retaining drying method for bamboo wood and application of the color-retaining drying method. In order to solve the technical problems that thermal oxidation browning, color degradation, yellowing and blackening are prone to occurring in the existing bamboo wood drying process, and the color retaining effect and the drying efficiency are difficult to consider at the same time, through ethyl alcohol replacement dehydration, oxidase inactivation and microorganism inhibition, vacuum low-oxygen and cold trap real-time water vapor trapping are matched, and the color retaining effect and the drying efficiency are improved. And chlorophyll degradation, enzymatic browning and oxidation yellowing are obviously slowed down, the natural color and luster of the bamboo wood are kept, meanwhile, the drying period is shortened, and the energy consumption and the cracking rate are reduced. The method is easy and convenient to operate, green, free of residues, excellent in color retaining effect, suitable for qiongzhuea bamboo wood, capable of being widely used for production of bamboo furniture, artware, building components and indoor decoration materials and good in industrial application prospect.