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Chlorophyll katabolism control associated protein of non-heading Chinese cabbage as well as encoding gene and application thereof

A technology for chlorophyll degradation and metabolism regulation, applied in non-heading Chinese cabbage chlorophyll degradation metabolism regulation related proteins and their coding genes and applications question

Inactive Publication Date: 2013-06-12
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

While many traits have been greatly improved, their limitations are becoming increasingly apparent

Method used

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  • Chlorophyll katabolism control associated protein of non-heading Chinese cabbage as well as encoding gene and application thereof
  • Chlorophyll katabolism control associated protein of non-heading Chinese cabbage as well as encoding gene and application thereof
  • Chlorophyll katabolism control associated protein of non-heading Chinese cabbage as well as encoding gene and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1, non-heading Chinese cabbage chlorophyll degradation metabolism regulation related protein coding genes BcNYE1 obtained.

[0024] 1.1 RNA extraction

[0025] Take about 0.1 g of aged non-heading Chinese cabbage leaf material. After the liquid nitrogen is fully ground, transfer to a 1.5ml centrifuge tube, add 1ml (Invitrogen Company), after mixing, place at room temperature for 15 minutes, add 0.2ml of chloroform:isoamyl alcohol (24:1), shake vigorously for 15 seconds, place at room temperature for 5 minutes, centrifuge at 13000rpm, 4°C for 15 minutes. Take the supernatant and add an equal volume of isopropanol, mix carefully, place at room temperature for 15 minutes, centrifuge at 13000 rpm, 4°C for 15 minutes. The precipitate was washed with 70% ethanol and dried at room temperature for 15 minutes. Dissolve in an appropriate amount of ddH treated with 0.1% DEPC 2 O water, stored at -80°C for later use.

[0026] 1.2 cDNA first-strand synthesis and rev...

Embodiment 2

[0047] Example 2: Functional analysis of proteins related to regulation of chlorophyll degradation in non-heading Chinese cabbage.

[0048] 2.1 Sequence comparison analysis

[0049] The homology of BcNYE1 with other species was analyzed by Genedoc software. The analysis results showed that BcNYE1 had great homology with NYE1 proteins of other species. SbSGR (sorghum, AAW82958), ZmSGR1 (maize, NP_001105770), ZmSGR2 (maize, NP_001105771), OsSGR (rice, EAZ09856), HvSGR (barley, AAW82955), AtNYE1 (Arabidopsis, NP_567673), AtNYE2 (Arabidopsis, ABD77556), LeSGR1 (tomato, AAY98500), GmSGR1 (soybean AAW82959), PsSGR (pea, BAF76352).

[0050] In order to analyze the relationship between BcNYE1 and NYE proteins of other species, the phylogenetic tree of BcNYE1 and NYE proteins of other species was established by using MEGA software. The results showed that BcNYE1 had the closest relationship with AtNYE1. The sequence accession numbers of the NYE proteins of each species in the phylo...

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Abstract

The invention belongs to the technical field of plant genetic engineering and particularly discloses a chlorophyll katabolism control associated protein of non-heading Chinese cabbage as well as an encoding gene and application thereof. The chlorophyll katabolism control associated protein provided by the invention is sourced from the non-heading Chinese cabbage with the variety of 'Xiadongqing',and has the name of BcNYE1 and the amino acid sequence shown as SEQIDNO:2. The encoding gene of the chlorophyll katabolism control associated protein of the non-heading Chinese cabbage has the nucleotide sequence shown as SEQIDNO:1 or the polynucleotide sequence of the amino acid sequence with the code of SEQIDNO:2. The gene disclosed by the invention can be applied to chlorophyll katabolism molecular mechanism research of the non-heading Chinese cabbage and improvement of plant stay-green property on the aspects of delaying blade chlorophyll degradation and blade senescence of plants.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to a protein related to the regulation of chlorophyll degradation metabolism and its encoding gene and application, in particular to a protein related to the regulation of chlorophyll degradation metabolism of non-heading Chinese cabbage, its encoding gene and its application. Background technique [0002] Chlorophyll is the main component for capturing light in photosynthesis. Chlorophyll is degraded on a large scale during the later stages of plant growth and development, leaf senescence or fruit ripening. Chlorophyll degradation is important for the recycling of protein nitrogen during leaf senescence (Hortensteiner S, Annu Rev Plant Biol, 57: 55-77, 2006); meanwhile, chlorophyll degradation is a detoxification method for senescent mesophyll cells (Matile et al Plant Physiology and Biochemistry, 27: 595-604, 1989; Matile et al. Plant Physiology, 112: ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10A01H5/00
Inventor 蒯本科邱凯魏强梁宁菁
Owner FUDAN UNIV
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