Method for improving cold resistance of red bract pineapples
A technology of cold resistance and pineapple, which is applied in the field of improving the cold resistance of red bud pineapple, can solve the problems of cold resistance of red bud pineapple plants without good cultivation measures, and achieve the effects of promoting photosynthesis, reducing damage, and alleviating degradation
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Embodiment 1
[0070] The present embodiment provides a kind of method that improves the cold resistance of red bud pineapple, comprises the following steps:
[0071] Step 1, add the tissue-cultured seedlings of red bud pineapple with a height of 3-4cm, 3-5 roots and 5-8 leaves to MS medium containing ABA for cultivation, and the control parameters of the cultivation process are: ABA is cultivated in the MS medium The final concentration in the medium was 8mg / L, the culture temperature was 25±2°C, the illuminance was 15001x, the humidity was 80%, and the culture time was 30 days.
[0072] Step 2, after the cultivation is completed, transfer to the artificial climate incubator for low temperature training treatment, the control parameters are: temperature 5°C, humidity 80%, light time 10h / d, illuminance 15001x, treatment for 5 days.
[0073] Determination of its appearance classification, chlorophyll content, soluble sugar and MDA content, soluble protein content, SOD / POD / CAT activity and the...
Embodiment 2
[0077] The present embodiment provides a kind of method that improves the cold resistance of red bud pineapple, comprises the following steps:
[0078] Step 1, add the tissue-cultured red bud pineapple seedlings with a height of 3 to 4 cm, 3-5 roots and 5-8 leaves to MS medium containing SA for cultivation, and the control parameters of the cultivation process are: SA is cultivated in the MS The final concentration in the medium is 5 mg / L, the culture temperature is 25±2° C., the light intensity is 1500 lx, the humidity is 80%, and the culture time is 20 days.
[0079] Step 2, after the cultivation is completed, transfer to the artificial climate incubator for low temperature training treatment, the control parameters are: temperature 5°C, humidity 80%, light time 10h / d, illuminance 15001x, treatment for 5 days.
[0080] Determination of its appearance classification, chlorophyll content, soluble sugar and MDA content, soluble protein content, SOD / POD / CAT activity and the cond...
Embodiment 3
[0084] The present embodiment provides a kind of method that improves the cold resistance of red bud pineapple, comprises the following steps:
[0085] Step 1, the red bract pineapple tissue culture seedling that height is 3~4cm, has 3-5 roots and 5-8 leaves is moved into containing CaCl 2 MS medium culture, the control parameters of the culture process are: CaCl 2 The final concentration in the MS medium is 5 mg / L, the culture temperature is 25±2° C., the illuminance is 1500 lx, the humidity is 80%, and the culture time is 40 days.
[0086] Step 2, after the cultivation is completed, transfer to the artificial climate incubator for low temperature training treatment, the control parameters are: temperature 5°C, humidity 80%, light time 10h / d, illuminance 15001x, treatment for 5 days.
[0087] Determination of its appearance classification, chlorophyll content, soluble sugar and MDA content, soluble protein content, SOD / POD / CAT activity and the conductivity of the tissue extr...
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