Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Chlorophyll degradation metabolism regulation related proteins of non-heading Chinese cabbage and their coding genes and application

A technology for chlorophyll degradation and metabolism regulation, which is applied in the field of chlorophyll degradation and metabolism regulation related proteins and their coding genes and applications. The chlorophyll degradation and metabolism regulation related proteins and their coding genes and applications of non-heading Chinese cabbage can solve the obvious limitations, etc. question

Inactive Publication Date: 2011-12-14
FUDAN UNIV
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While many traits have been greatly improved, their limitations are becoming increasingly apparent

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chlorophyll degradation metabolism regulation related proteins of non-heading Chinese cabbage and their coding genes and application
  • Chlorophyll degradation metabolism regulation related proteins of non-heading Chinese cabbage and their coding genes and application
  • Chlorophyll degradation metabolism regulation related proteins of non-heading Chinese cabbage and their coding genes and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1, non-heading Chinese cabbage chlorophyll degradation metabolism regulation related protein coding genes BcNYE1 obtained.

[0024] 1.1 RNA extraction

[0025] Take about 0.1 g of aged non-heading Chinese cabbage leaf material. After the liquid nitrogen is fully ground, transfer to a 1.5ml centrifuge tube, add 1ml (Invitrogen Company), after mixing, place at room temperature for 15 minutes, add 0.2ml of chloroform:isoamyl alcohol (24:1), shake vigorously for 15 seconds, place at room temperature for 5 minutes, centrifuge at 13000rpm, 4°C for 15 minutes. Take the supernatant and add an equal volume of isopropanol, mix carefully, place at room temperature for 15 minutes, centrifuge at 13000 rpm, 4°C for 15 minutes. The precipitate was washed with 70% ethanol and dried at room temperature for 15 minutes. Dissolve in an appropriate amount of ddH treated with 0.1% DEPC 2 O water, stored at -80°C for later use.

[0026] 1.2 cDNA first-strand synthesis and rev...

Embodiment 2

[0047] Example 2: Functional analysis of proteins related to regulation of chlorophyll degradation in non-heading Chinese cabbage.

[0048] 2.1 Sequence comparison analysis

[0049] The homology of BcNYE1 with other species was analyzed by Genedoc software. The analysis results showed that BcNYE1 had great homology with NYE1 proteins of other species. SbSGR (sorghum, AAW82958), ZmSGR1 (maize, NP_001105770), ZmSGR2 (maize, NP_001105771), OsSGR (rice, EAZ09856), HvSGR (barley, AAW82955), AtNYE1 (Arabidopsis, NP_567673), AtNYE2 (Arabidopsis, ABD77556), LeSGR1 (tomato, AAY98500), GmSGR1 (soybean AAW82959), PsSGR (pea, BAF76352).

[0050] In order to analyze the relationship between BcNYE1 and NYE proteins of other species, the phylogenetic tree of BcNYE1 and NYE proteins of other species was established by using MEGA software. The results showed that BcNYE1 had the closest relationship with AtNYE1. The sequence accession numbers of NYE proteins of each species in the phylogene...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of plant genetic engineering and particularly discloses a chlorophyll katabolism control associated protein of non-heading Chinese cabbage as well as an encoding gene and application thereof. The chlorophyll katabolism control associated protein provided by the invention is sourced from the non-heading Chinese cabbage with the variety of 'Xiadongqing',and has the name of BcNYE1 and the amino acid sequence shown as SEQIDNO:2. The encoding gene of the chlorophyll katabolism control associated protein of the non-heading Chinese cabbage has the nucleotide sequence shown as SEQIDNO:1 or the polynucleotide sequence of the amino acid sequence with the code of SEQIDNO:2. The gene disclosed by the invention can be applied to chlorophyll katabolism molecular mechanism research of the non-heading Chinese cabbage and improvement of plant stay-green property on the aspects of delaying blade chlorophyll degradation and blade senescence of plants.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and specifically relates to a protein related to the regulation of chlorophyll degradation metabolism and its encoding gene and application, in particular to a protein related to the regulation of chlorophyll degradation metabolism of non-heading Chinese cabbage, its encoding gene and its application. Background technique [0002] Chlorophyll is the main component for capturing light in photosynthesis. Chlorophyll is degraded on a large scale during the later stages of plant growth and development, leaf senescence or fruit ripening. Chlorophyll degradation is important for the recycling of protein nitrogen during leaf senescence (Hortensteiner S, Annu Rev Plant Biol, 57: 55-77, 2006); meanwhile, chlorophyll degradation is a detoxification method for senescent mesophyll cells (Matile et al Plant Physiology and Biochemistry, 27: 595-604, 1989; Matile et al. Plant Physiology, 112: ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10A01H5/00
Inventor 蒯本科邱凯魏强梁宁菁
Owner FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com