Method for synthesis, transfection and detection of anti-aging gene of goat

A detection method and anti-aging technology, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve the problem of high fish oil and achieve the effect of alleviating cardiovascular disease

Inactive Publication Date: 2015-07-01
QINGDAO AGRI UNIV
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Problems solved by technology

However, the proportion of n-6 and n-3 in the current diet is too hi

Method used

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  • Method for synthesis, transfection and detection of anti-aging gene of goat
  • Method for synthesis, transfection and detection of anti-aging gene of goat
  • Method for synthesis, transfection and detection of anti-aging gene of goat

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Embodiment Construction

[0037] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0038] Used equipment and tools thereof in the embodiment of the present invention are:

[0039] The eukaryotic expression vector pcDNA3.1(+)-FatI vector was constructed by our laboratory. Genome extraction kit (DP304-02) and plasmid extraction kit (DP103-02) were purchased from Tiangen Technology Co., Ltd. (Beijing, China). Restriction enzyme SalI (D1080B), LA Taq (RR02MA), rTaq (R001A), DNA molecular weight standards (D501A and D502A), reverse transcription kit (DRR047A), purchased from Treasure Biotechnology Co., Ltd. (Dalian, China) . Gel recovery kit (A9281) was purchased from Promega (Promega, USA). G418 (1720) and BrdU monoclonal anti...

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Abstract

The invention discloses a method for synthesis, transfection and detection of an anti-aging gene of a goat. According to the method, by adopting a transgenic technology, a transgenic Boer goat fibroblast carrying an n-3 Fat I gene is obtained, and fatty acid determination analysis shows that the transgenic fibroblast can convert n-6 polyunsaturated fatty acid into n-3 polyunsaturated fatty acid. By virtue of comparative analysis on the transgenic fibroblast and trans-pEGFP-N1 cells in the aspects of cell proliferation, cell apoptosis and cell aging, a conclusion that the Fat I gene has an anti-aging effect in the transgenic fibroblast of the goat is obtained. The research of the method disclosed by the invention provides an indispensable condition for effective and low-cost production of an egg-milk-meat product containing n-3 polyunsaturated fatty acid from livestock and poultry animals with the n-3 Fat I gene and the livestock and poultry obtaining self-synthesized n-3 polyunsaturated fatty acid.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a synthesis, transfection and detection method of a goat anti-aging gene. Background technique [0002] n-3 polyunsaturated fatty acids (PUFAs for short) are important components of cell membranes and play an important role in the prevention and control of many human diseases. But for mammals, due to the lack of fatty acid dehydrogenase that can convert n-6PUFAs into n-3PUFAs in their body, they cannot be synthesized endogenously, but the increasingly mature transgenic technology makes it possible to produce transgenes that can synthesize n-3PUFAs by themselves Animals become possible. But transgenic technology itself may bring some negative effects to transgenic animals, for example, cell senescence and so on. Therefore, if one wants to identify the impact of the transferred foreign gene on cell aging and other aspects, the impact of the transgenic technology itself must be...

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Application Information

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IPC IPC(8): C12N15/85C12N15/53C12N5/10
Inventor 孙晓凤李兰程顺峰沈伟
Owner QINGDAO AGRI UNIV
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