Kit for simultaneous detection of four depression markers and preparation method thereof

Abstract

The invention relates to the technical field of immunodetection, and provides a kit for simultaneous detection of four depression markers and a preparation method thereof, the preparation method is as follows: antibody coating is prepared; enzyme label plates prepared in the first step are respectively poured, closed, washed, air-dried, and stored for standby use; a sample is loaded, the enzyme label plates are put into an incubation oscillator for incubation, then a detection antibody is added; enzyme SA HRP is added into plate holes for continuing incubation; CCD imager is used for filming and imaging, the image IDV value can be calculated by combination of image analysis software, according to the IDV value, a standard curve is drawn, and the sample concentration result is calculated; and softMax regression model and Excel table are used for calculation and statistical analysis of the sample concentration result. The preparation method solves effectively the problem of complicated process, operation disadvantage and low sensitivity.

Description

technical field [0001] The invention relates to the technical field of immune detection, in particular to a kit for simultaneously detecting four depression markers and a preparation method thereof. Background technique [0002] In recent years, more and more evidences have proved the role of inflammatory response in the occurrence and development of depression. Many inflammatory factors and corresponding soluble receptors, chemotactic molecules, adhesion molecules, acute phase proteins, etc. Peripheral blood and cerebrospinal fluid in patients with depression significantly increased, and the relationship between individual symptoms of depression, such as fatigue, cognitive impairment, and sleep disturbance, and inflammatory factors has also been continuously reported. These depression-related biomarkers can be used to assist clinical diagnosis and prognosis evaluation, and will provide biological evidence for the objective diagnosis of depression. Further, we used more tha...

AI Technical Summary

Problems solved by technology

However, in the usual enzyme-linked immunosorbent assay (ELISA) detection of samples, each detection well generally requires 100-200 μl, and the sample volume is relatively large. In addition, there are more than one biomarkers of depression, which requires joint monitoring of multiple factors for diagnosis. If you still rely on a single ELISA test, the sample size will be too large; it is obviously time-consuming and laborious to test each factor individually to assist the diagnosis of depression, and the cost of diagnosis is high, which is generally unaffordable for patients

[0004] RIA technology, the radioimmunoassay method, has both high specificity of immune response and high sensitivity of radioactivity measurement, but there are problems such as radiation radiation and pollution, and it also poses a threat to the health of testing personnel, and the operation is relative to ELISA Complicated, only one indicator can be measured in one inspection, and a special radioactive experiment site needs to be provided, which requires high investment and great harm

[0005] Although the protein chip can realize the function of simultaneous detection of multiple factors, it is because it coats the antibody on the substrate slide, microsphere or membrane used to prepare the protein chip, and the substrate slide and membrane must be aldehyde-treated before use. Kylation or poly-lysine and other pretreatments lead to increased production costs, and the follow-up experiments are cumbersome, poor repeatability, and difficult to promote

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