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Diabetic nephropathy early screening kit, biomarker detection method and application

A technology for diabetic nephropathy and kits, which can be used in biological tests, material inspection products, etc., can solve the problems of complications, high trauma and high technical difficulty.

Inactive Publication Date: 2016-08-24
BIO TECH ACADEMY CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although renal biopsy can assist in the early diagnosis of diabetic nephropathy, the technique is invasive and will cause complications after operation, and it is technically difficult, so it cannot be used as a routine item in the examination of patients with diabetic nephropathy

Method used

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  • Diabetic nephropathy early screening kit, biomarker detection method and application
  • Diabetic nephropathy early screening kit, biomarker detection method and application
  • Diabetic nephropathy early screening kit, biomarker detection method and application

Examples

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Embodiment 1

[0102] Example 1: Screening and verification of biomarkers for early diagnosis of diabetic nephropathy

[0103] 1. Experimental method

[0104] 1. Pathological screening

[0105] A total of 205 subjects with known physical conditions were selected, including 50 healthy controls and 155 patients with type 2 diabetes (all patients had diabetes for more than 2 years); 155 diabetic patients were divided according to proteinuria , including 61 cases of normal proteinuria, 52 cases of microalbuminuria, 42 cases of massive proteinuria. The MDRD GRF formula estimates glomerular filtration rate (eGFR).

[0106] Note: This study has been approved by the ethics review agency, and all subjects signed informed consent.

[0107] 2. Collection and processing of urine samples

[0108] The first urine sample (30 mL) excreted by the subject in the morning was collected in a sterile container, centrifuged at 8000 g for 30 min at 4°C, and the supernatant was collected and stored at -80°C for ...

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Abstract

A diabetic nephropathy early screening reagent kit comprising testing reagents. The testing reagents comprise a first testing reagent, a second testing reagent, a third testing reagent, and a fourth testing reagent. A solute for the first testing reagent is a first podocalyxin antibody. A solute for the second testing reagent is a first collagen IV antibody. A solute for the third testing reagent is a first liver fatty acid binding protein antibody. A solute for the fourth testing reagent is a first neutrophil gelatinase-associated lipocalin antibody. By having podocalyxin, collagen IV, liver fatty acid binding protein, and neutrophil gelatinase-associated lipocalin serving as biomarkers, the diabetic nephropathy early screening reagent kit is capable of providing assistance in determining kidney damages on the basis of an actual testing result, thus providing assistance in distinguishing early-stage diabetic nephropathy.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a kit for early screening of diabetic nephropathy, a method for detecting biomarkers and an application thereof. Background technique [0002] Diabetic nephropathy is one of the most serious chronic microvascular complications of diabetes, which eventually leads to end-stage renal failure and is the main cause of death in diabetic patients. According to the statistics of the World Health Organization in 2013, about 347 million people in the world suffer from diabetes, and more than 80% of the deaths from diabetes occur in developing countries. At the same time, the World Health Organization predicts that by 2030, the number of deaths from diabetes worldwide will double, of which 30% to 40% of type 2 diabetes patients will develop diabetic nephropathy, and 20% to 40% of type 1 diabetes patients will develop diabetic nephropathy after 15 years. ~30 years later, diabetic nephrop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68
CPCG01N33/68
Inventor 张贯京张俊然谷东风克里斯基捏·普拉纽克艾琳娜·古列莎波达别特·伊万刘遥峰卢卡·穆桑特杨轶石江波朱孔林
Owner BIO TECH ACADEMY CHINA
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