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Morusin anti-tumor application

A kind of use and malignant tumor technology, applied in the direction of antineoplastic drugs, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems that are not enough to prove the anticancer effect of Sangsin

Inactive Publication Date: 2015-07-29
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The current experimental results are all simple in vitro MTT cytotoxicity experiments, which are not enough to confirm the anticancer effect of Sangsin

Method used

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  • Morusin anti-tumor application

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0065] Experimental Example 1 Moranginin of the present invention has inhibitory effect on tumor proliferation

[0066] Adherent tumor cells in the logarithmic growth phase and non-adherent suspension cultured tumor cells (tumor stem cells) Hela (cervical cancer cells), GBM (glioma cells) and human normal liver cells L02 were taken and digested by 2×10 3 Inoculate each well in a 96-well plate, culture 100 μl per well for 24 hours, and treat tumor cells, tumor stem cells and normal human liver cells with 200 μl of medium containing 0.5, 1, 2, 4, 8, 16 μg / mL Sangsin , the control group was not treated, and each dose group had 3 replicate wells, placed a 96-well plate in the incubator for 72 hours or 1, 2, 3, 4, and 5 days, then added 10 μl MTT to each well, continued to cultivate for 4 hours, and then added 100 μl 10% SDS / 0.01N HCL, overnight in the incubator, measure the OD value with a microplate reader (λ=570nm), and take the average value. Calculate the cell survival rate, ...

experiment example 2

[0070] Experimental example 2 Sangsin has the effect of reducing stemness phenotype on human malignant glioma stem cells

[0071] Western blot was used to detect the effect of Sangsin on the expression of stemness-related proteins in tumor stem cells. Human glioma tumor stem cells were divided into 2×10 6Inoculated in agarose-coated culture dish, cultured for 24 hours, treated with 0.5, 1, 2 μg Morocin for 48 hours respectively; collected cells RIPA lysate to extract protein, separated by SDS-PAGE, transferred to membrane, added anti-CD133, nestin , Sox2, and Oct4 were incubated with primary antibodies, incubated with secondary antibodies (HRP-labeled goat anti-rabbit Ig), developed with chemiluminescent substrates, and analyzed with Quantity One 4.5.0 software: Relative protein expression = target protein strip The gray value of the band / the gray value of the β-actin band; the experiment was repeated 3 times.

[0072] result:

[0073] ① Treating human glioma stem cells with...

experiment example 3

[0074] Experimental example 3 Sangsin has lateral differentiation effect on human tumor cells and tumor stem cells

[0075] (1) Oil Red O staining to detect intracellular lipid droplets

[0076] Adherent tumor cells in the logarithmic growth phase and non-adherent suspension culture tumor cells (tumor stem cells), malignant glioma cells), GBM stem cells, human breast cancer cells (MDA-MB-231, MCF-7) and stem cells Tumor (MCF-7M) was digested by 2×10 5 Each well was inoculated in a 24-well plate, 200 μl per well was cultured for 24 hours, tumor cells and tumor stem cells were treated with 500 μl of medium containing 0.5, 1, and 2 μg / mL Sangsin for 3 days, and then cultured with the same drug dose. liquid culture for 3 days. The collected cells were washed 3 times with PBS (pH 7.6), fixed with 100% methanol (-20°C) for 10 minutes, DH 2 Wash 3 times with O, stain with Oil Red O at room temperature for 30 minutes, wash with water, stain with Mayer's hematoxylin, and observe red...

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Abstract

The invention provides an application of morusin or its pharmaceutically acceptable derivative in preparation of medicines for resisting malignant tumor. The invention also provides an application of morusin or its pharmaceutically acceptable salt in preparation of anti-glioma medicines. It is found through researches that morusin shows a good activity of resisting malignant tumor (such as glioma) in vitro and in vivo. In addition, it shows through experiments that morusin has a good effect of suppressing tumor growth and inducing transversal differentiation and apoptosis of tumor cells and tumor stem cells, and has little toxic and side effect on normal cells. The morusin has a good application prospect.

Description

technical field [0001] The present invention relates to the new anti-tumor application of Sangshin. Background technique [0002] Malignant tumor, namely cancer, is the first or second deadliest disease in the world, and its morbidity and mortality are constantly increasing, seriously threatening human health and life. Malignant tumor cells grow diffusely and aggressively. Surgical resection of tumor lesions, postoperative radiotherapy and chemotherapy can reduce the tumor volume and significantly alleviate the condition, but long-term curative effect cannot be obtained. [0003] At present, malignant tumors are mostly treated by killing tumor cells, inducing tumor cell differentiation, and inducing tumor cell apoptosis. Among them, drugs that induce tumor cell differentiation are still relatively rare. In recent years, a large number of studies have shown that almost all tumor tissues have their own stem cells, namely cancer stem cells (CSCs), which are related to biologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P35/00
Inventor 王修杰
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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