Hybridoma cell line secreting anti-wheat yellow mosaic virus monoclonal antibody and application of monoclonal antibody

A technology of hybridoma cell line and wheat mosaic virus, applied in antiviral immunoglobulin, analytical materials, biochemical equipment and methods, etc., can solve unseen problems

Active Publication Date: 2017-12-29
ZHEJIANG UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, these serological methods established with polyantibodies have not been reported in the large-scale detection of field samples due to their shortcomings.

Method used

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  • Hybridoma cell line secreting anti-wheat yellow mosaic virus monoclonal antibody and application of monoclonal antibody
  • Hybridoma cell line secreting anti-wheat yellow mosaic virus monoclonal antibody and application of monoclonal antibody

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Embodiment Construction

[0015] Secreting anti-wheat yellow mosaic virus monoclonal antibody hybridoma cell 4E11, which can secrete specific anti-wheat yellow mosaic virus monoclonal antibody, was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on March 26, 2015, address: Beijing No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Zip Code: 100101, and the preservation number is CGMCC No.10415;

[0016] Anti-wheat yellow mosaic virus monoclonal antibody ascites ELISA titer reaches 10 -7 , the antibody type and subclass are IgG1, kappa chain, the monoclonal antibody can have a specific immune binding reaction with the capsid protein of wheat yellow mosaic virus, and the sensitivity of the monoclonal antibody to detect diseased leaves reaches 1:40960 times dilution;

[0017] Monoclonal antibodies against wheat yellow mosaic virus are only specific for wheat yellow mosaic virus, but not for Chinese wheat mosaic virus, barley yellow mosaic viru...

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Abstract

The invention discloses a hybridoma cell strain secreting monoclonal antibody against wheat yellow mosaic virus (WYMV) and application of the monoclonal antibody. Virus particles with WYMV purified are used as an antigen to immunize BALB / c mice; through cell fusion, screening and cloning, one strain of hybridoma cell strain 4E11 capable of stable secreting of anti-WYMV monoclonal antibodies is obtained; and the strain has a preservation number CGMCC No.10415. The monoclonal antibodies secreting by the cell line react with WYMV capsid protein, the secrete monoclonal antibody ascites ELISA titer reaches more than 10<-7>, and the antibody type and subtype is IgG1, kappa chain. The monoclonal antibodies secreting by 4E11 have specific reaction with WYMV, but does not react with Chinese wheat mosaic virus, barley yellow mosaic virus, and wheat mosaic rosette virus. The hybridoma cell strain and monoclonal antibody secreted by the cell strain provide material support for the establishment of diagnosis, inspection and quarantine, and scientific prevention and control of the wheat virus.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell line secreting anti-wheat yellow mosaic virus monoclonal antibody and the application of the monoclonal antibody. Background technique [0002] Wheat yellow mosaic disease, also known as "spindle mosaic disease", is an important soil-borne virus disease in wheat production in my country. Wheat yellow mosaic is caused by wheat yellow mosaic virus (WYMV). Before the 1960s in my country, the virus disease only occurred sporadically. In the 1970s, due to the large-scale planting of susceptible varieties, the disease occurred and became popular in my country's wheat planting areas. At present, the disease is mainly distributed in the Yangtze River, the middle and lower reaches of the Yellow River and the Huaihe River Basin in my country. , including Sichuan, Shaanxi, Jiangsu, Zhejiang, Anhui, Hubei, Shandong and Henan provinces, including the Lixia River area and Ningzhen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/20C07K16/10G01N33/577G01N33/569
Inventor 周雪平吴建祥李娜
Owner ZHEJIANG UNIV
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