Cedrela sinensis polysaccharide extracting method

A technology of polysaccharide extraction and toona seeds, which can be used in pharmaceutical formulations, metabolic diseases, organic active ingredients, etc., and can solve problems such as inconvenience

Active Publication Date: 2015-08-26
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In summary, there are obviously inconveniences and defects in

Method used

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  • Cedrela sinensis polysaccharide extracting method
  • Cedrela sinensis polysaccharide extracting method
  • Cedrela sinensis polysaccharide extracting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Step A: Degreasing the pre-selected Chinese toon seeds to remove fat-soluble components. Specifically, the above-mentioned Chinese toon seeds can be degreased by 95% ethanol or petroleum ether.

[0038] Preferably, before the degreasing treatment of the Chinese toon seeds in step A, the Chinese toon seeds can be pulverized, so that the Chinese toon seeds have a higher degreasing rate.

[0039] Step B: extract the degreased Chinese toon seeds by adding water to reflux, and perform suction filtration and decompression treatment to obtain a concentrated solution. In this step, the solid-liquid ratio of adding water and reflux extraction is 1:13g / ml, the extraction time is 119min, and the number of extractions is 3 times.

[0040] Step C: Deproteinizing the concentrated solution to obtain a deproteinized solution. The present invention preferably adopts the sevage method to deproteinize.

[0041] Step D: adding ethanol to the deproteinized solution, and controlling the e...

Embodiment 2

[0045] Step A: Degreasing the pre-selected Chinese toon seeds to remove fat-soluble components. Specifically, the above-mentioned Chinese toon seeds can be degreased by 95% ethanol or petroleum ether.

[0046] Preferably, before the degreasing treatment of the Chinese toon seeds in step A, the Chinese toon seeds can be pulverized, so that the Chinese toon seeds have a higher degreasing rate.

[0047] Step B: extract the degreased Chinese toon seeds by adding water to reflux, and perform suction filtration and decompression treatment to obtain a concentrated solution. In this step, the solid-liquid ratio of adding water and reflux extraction is 1:12g / ml, the extraction time is 120min, and the extraction times are 3 times.

[0048] Step C: Deproteinizing the concentrated solution to obtain a deproteinized solution. The present invention preferably adopts the sevage method to deproteinize.

[0049] Step D: adding ethanol to the deproteinized solution, and controlling the ethan...

Embodiment 3

[0053] Step A: Degreasing the pre-selected Chinese toon seeds to remove fat-soluble components. Specifically, the above-mentioned Chinese toon seeds can be degreased by 95% ethanol or petroleum ether.

[0054] Preferably, before the degreasing treatment of the Chinese toon seeds in step A, the Chinese toon seeds can be pulverized, so that the Chinese toon seeds have a higher degreasing rate.

[0055] Step B: extract the degreased Chinese toon seeds by adding water to reflux, and perform suction filtration and decompression treatment to obtain a concentrated solution. In this step, the solid-liquid ratio of adding water and reflux extraction is 1:11g / ml, the extraction time is 122min, and the extraction times are 3 times.

[0056] Step C: Deproteinizing the concentrated solution to obtain a deproteinized solution. The present invention preferably adopts the sevage method to deproteinize.

[0057] Step D: adding ethanol to the deproteinized solution, and controlling the ethan...

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Abstract

The invention is applied to the technical field of the natural product extracting process, and provides a cedrela sinensis polysaccharide extracting method. The method comprises the following steps: A: conducting degreasing treatment on cedrela sinensis to remove liposoluble constituents; B: adding water into the degreased cedrela sinensis to conduct reflux extraction, and conducting extraction filtration and decompression treatment to obtain a concentrate; C: conducting deproteinization treatment on the concentrate to obtain a deproteinized solution; D: adding ethyl alcohol into the deproteinized solution to control the content of ethyl alcohol to be 70-90%; E: leaving a mixed solution consisting of ethyl alcohol and the deproteinized solution to stand at 1-5 DEG C for a preset period of time; F: conducting extraction filtration treatment on the mixed solution subjected to standing treatment, washing with absolute ethyl alcohol, and then conducting drying treatment to obtain the cedrela sinensis polysaccharide. According to the method, the polysaccharide in the cedrela sinensis can be efficiently extracted, the polysaccharide has the efficacies of immunoregulation, inflammation resistance, oxidation resistance and the like, and the toxicity is relatively low.

Description

technical field [0001] The invention relates to the technical field of natural product extraction technology, in particular to a method for extracting polysaccharides from Toona sinensis. Background technique [0002] Toona sinensis is the fruit of the genus Toona sinensis in the Meliaceae family. Toon, first recorded in "Tang Materia Medica" [1] , widely distributed in my country, is a well-known homologous plant of medicine and food, and its root bark, bark, buds, leaves, and fruits can be used as medicine. Toona sinensis is a traditional Chinese medicinal material in my country. It has the effects of expelling wind, dispelling cold, and relieving pain. disease [1-2] . [0003] At present, studies have found that Toona sinensis contains active ingredients such as phenols, tannins, alkaloids, saponins, steroids, terpenes, volatile oils and oils. [3-6] . Polysaccharides have special effects such as immune regulation, anti-oxidation, lowering blood sugar and blood lipids...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P37/02A61P39/06A61P3/10A61P3/06A61P29/00A61P39/00
Inventor 李万忠丁世洪刘兵
Owner WEIFANG MEDICAL UNIV
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