Application of hericium erinaceus mutant strain H07 in preparation of memory improvement medicine

A technique for mutagenizing bacterial strains and Hericium erinaceus, applied in drug combinations, methods based on microorganisms, fungi, etc., to increase the number of times, significant medical effects, and improve memory

Inactive Publication Date: 2015-09-02
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although senile dementia drugs have been marketed at home and abroad, there is no drug or health product on the market that uses Hericium erinaceus mutagenized strain H07 mycelium extract as a drug or health product to improve memory and cognitive function

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Grind 1000g of Hericium erinaceus mutagenic strain H07 mycelium extract (strain number: CCTCC NO: M 2014669), pass through a 100-mesh sieve, and extract with water for 3 time, 4 hours for the first time, 3 hours for the second time, and 2 hours for the third time, combine the three water extracts, centrifuge at 4000 rpm for 5 minutes, discard the precipitate, and then filter with a 200-mesh filter cloth to obtain the filtrate. The filtrate was concentrated under reduced pressure to a relative density of 1.00 (70°C), and the concentrated solution was freeze-dried at -30°C for 72 hours to obtain 93.40 g of mycelia extract of Hericium erinaceus mutagenized strain H07.

Embodiment 2

[0019] Crush 1000g of Hericium erinaceus mutagenic strain H07 mycelium extract (strain number: CCTCC NO: M 2014669), pass through a 100-mesh sieve, and extract with water 3 times at a solid-liquid mass ratio of 1:60 at a temperature of 80°C , 3 hours for the first time, 2 hours for the second time, and 1 hour for the third time, combine the three water extracts, centrifuge at 5000 rpm for 10 minutes, discard the precipitate, and then filter with a 300-mesh filter cloth to obtain the filtrate. The solution was concentrated under reduced pressure to a relative density of 1.20 (80°C), and the concentrated solution was freeze-dried at -60°C for 48 hours to obtain 101.91 g of mycelia extract of Hericium erinaceus mutagenized strain H07.

[0020] In order to prove whether the mycelia extract of Hericium erinaceus mutagenized strain H07 of the present invention has the function of improving memory and improving cognitive function, the new application will be shown by the following e...

experiment example 1

[0022] Mouse Morris water maze (Morris water maze, MWM) experiment

[0023] The Hericium erinaceus mutagenic strain H07 mycelia extract prepared in Example 2 is made into low-dose group, middle-dose group, high-dose group with concentration of 0.3g / kg, 1g / kg, 3g / kg with deionized water dose group. The oral gavage dose of the tested mice, that is, the oral gavage dose of the 20±2g body weight test mice was 0.2mL / 20g, while the blank control group was orally gavaged with an equal volume of normal saline, every day Both were administered by intragastric administration, and after continuous intragastric administration for 7 days, the mice were placed in a water maze tester, and after training for several times, the time for the mice in each group to find the platform was measured. Set 4 blind ends in the water maze tester (Morris), which has been widely recognized in the world. According to the order from near to far from the exit, it is 4 areas, 3 areas, 2 areas, and 1 areas. ...

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Abstract

The invention provides application of hericium erinaceus mutant strain H07 in preparation of memory improvement medicine. The hericium erinaceus mutant strain H07 is filed in China Center for Type Culture Collection (CCTCC address: Wuhan University in Hubei province, China) in Dec, 26, 2014, by name of Hericium erinaceus H07 with the CCTCC No: M 2014669. The mycelium extract of the hericium erinaceus mutant strain H07 has the remarkable medical treatment effect in medical applications in improving memory and cognitive function.

Description

Technical field [0001] The present invention provides the use of H07 Monkey -Garbolin mutagenic strain in preparation and improvement of memory drugs. It belongs to the field of traditional Chinese medicine health care products. It is specific to involve High -yield mutagne strain H07 mobilizer extractFunctional drugs. Background technique [0002] At present, the commonly used drugs for treatment of dementia on the market are mainly acetylcholine inhibitors, such as: he Klin, Dona, etc., but the toxic and side effects are large and expensive.The performance, medicinal fungi attaches great importance to the overall regulation and balance, and has the advantages of small toxic side effects and wide distribution.According to "Chinese Medicinal Fungus", monkey heads are flat, sweet, can benefit the five internal organs, help digestion, nourish, anti -cancer, and treat neurasthenia.According to reports, the nutritional ingredients contained in monkey fungus contain rich protein, fat,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A61K36/06A61P25/28C12R1/645
Inventor 王迪逯家辉张峻榕刘洋滕利荣孟庆繁孟令军权宇彤周毓霖
Owner JILIN UNIV
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