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Halomonas axialensis and application thereof in degrading of decabromodiphenyl ether

A technology of decabromodiphenyl ether and Halomonas, which is applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problem of less aerobic degradation of decabromodiphenyl ether, and broaden the degradation pathway Effect

Active Publication Date: 2015-09-09
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are few studies on the aerobic degradation of decabromodiphenyl ether

Method used

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  • Halomonas axialensis and application thereof in degrading of decabromodiphenyl ether
  • Halomonas axialensis and application thereof in degrading of decabromodiphenyl ether
  • Halomonas axialensis and application thereof in degrading of decabromodiphenyl ether

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Effect test

Embodiment 1

[0024] 1. Isolation and identification of Halomonas axialensis SWIR-CL71

[0025] 1. Enrichment, isolation and purification of Halomonas axialensis SWIR-CL71

[0026] Medium:

[0027] Artificial Seawater Medium (MMC): Each liter of medium contains 24g of NaCl, NH 4 NO 3 1g, KCl 0.7g, KH 2 PO 4 2.0g, Na 2 HPO 4 3.0g, pH 7.4, MgSO 4 7g (added after sterilization).

[0028] Trace element salt solution: every liter of trace element salt solution contains 2mg of CaCl, FeCl 3 ·6H 2 O 50mg, CuSO 4 0.5mg, MnCl 2 ·.H 2 O 0.5mg, ZnSO 4 ·.7H 2 O 10 mg.

[0029] Marine bacteria agar 2216 medium and marine bacteria broth 2216 medium were purchased from BD Medical Instruments (Shanghai) Co., Ltd.

[0030] Decabromodiphenyl ether mother liquor: the organic solvent is dichloromethane, and the concentration of decabromodiphenyl ether is 1 g / L.

[0031] The axial seamount Halomonas (Halomonas axialensis) SWIR-CL71 of the present invention is obtained from the surface sedim...

Embodiment 2

[0043] Degradation analysis of decabromodiphenyl ether by Halomonas axialensis SWIR-CL71:

[0044] Artificial Seawater Medium (MMC): Each liter of medium contains 24g of NaCl, NH 4 NO 3 1g, KCl 0.7g, KH 2 PO 4 2.0g, Na 2 HPO 4 3.0g, pH 7.4, MgSO 4 7g (added after sterilization).

[0045] Trace element salt solution: every liter of trace element salt solution contains 2mg of CaCl, FeCl 3 ·6H 2 O 50mg, CuSO 4 0.5mg, MnCl 2 ·.H 2 O 0.5mg, ZnSO 4 ·.7H 2 O 10 mg.

[0046] Enrichment medium EM (Enrichment Medium): marine bacteria broth 2216 medium.

[0047] Decabromodiphenyl ether degradation medium: 100mL MMC medium + trace elements.

[0048] Proliferation culture of Halomonas axialensis (Halomonas axialensis) SWIR-CL71: Under sterile conditions, inoculate the strain of Halomonas axialensis into a sterilized 1L medium containing 500mL seawater broth culture (2216) Place in a conical flask in a constant temperature shaker at 28°C, 160rpm, and incubate for 48h. ...

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Abstract

The invention provides halomonas axialensis and application of the halomonas axialensis in degrading of decabromodiphenyl ether, and relates to the field of microbe application. The halomonas axialensis SWIR-CL 71 has a collection number of CCTCC No.M2015211 and can be applied to the degrading of decabromodiphenyl ether. After the halomonas axialensis SWIR-CL 71 is cultured for 8 days in a degrading culture medium using decabromodiphenyl ether as the unique carbon source under the conditions that the pH is 7.4, the temperature is 28 DEG C, and the rotating frequency of a shake bed is 160rpm, the degrading rate of the halomonas axialensis for decabromodiphenyl ether is up to 87%.

Description

technical field [0001] The invention relates to the field of microorganism application, in particular to halomonas seamount and its application in degrading decabromodiphenyl ether. Background technique [0002] Polybrominated diphenyl ethers (PBDEs) are a series of aromatic compounds containing bromine atoms. According to the number and position of bromine atoms on the benzene ring, there are a total of 209 isomers of polybrominated diphenyl ethers, and its biggest use is to be added to composite materials as a flame retardant to improve fire resistance. At present, PBDEs have been widely used in commercial products such as electronic and electrical equipment, automatic control equipment, building materials and textiles. Three brominated flame retardants are mainly used commercially: pentabromodiphenyl ether (penta-BDE), octabromodiphenyl ether (octa-BDE) and decabromodiphenyl ether (deca-BDE). Pentabromodiphenyl ether (penta-BDE) and octabromodiphenyl ether (octa-BDE) pe...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02
CPCA62D3/02A62D2101/28C12N1/20C12N1/205C12R2001/01
Inventor 邵宗泽王万鹏蔡庆涛
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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