Application of chitosan oligosaccharide in preparing vaccine adjuvant and vaccine composition

Abstract

The invention discloses an application of chitosan oligosaccharide in preparing a vaccine adjuvant as well as a vaccine composition and a vaccine preparation containing the vaccine adjuvant. The vaccine adjuvant comprises chitosan oligosaccharide. The method for preparing the chitosan oligosaccharide of which the polymerization degree is 2-20 comprises the following steps: performing biological enzymatic hydrolysis, chemical decomposition or physical method decomposition on chitosan, wherein the deacetylation degree of the chitosan oligosaccharide of which the polymerization degree is 2-20 is greater than 90%, the molecular weight is 400-5000Da, and the sum of components of which the polymerization degrees are 3-10 is not less than 50%. The vaccine adjuvant containing chitosan can be used as an adjuvant of an attenuated vaccine, a nucleic acid vaccine, a polypeptide vaccine or protein vaccine.

Description

technical field

[0001] The invention belongs to the technical field of medicine, and particularly relates to the use of chitosan oligosaccharide in preparing vaccine adjuvants and vaccine compositions and vaccine preparations containing the vaccine adjuvants. Background technique

[0002] Adjuvant is a kind of substance that can enhance the immune effect of antigen when applied in advance of antigen or at the same time with antigen. Its main function is to enhance the specific immune response of the body's immune system to antigen. Since the British doctor Jenner vaccinated against smallpox more than 200 years ago and achieved success in human trials, humans can prevent and control certain infectious diseases through vaccination. In 1925, French immunologist and veterinary scientist Gaston Ramon discovered that adding tapioca starch, agar, pyogenic bacteria and other substances unrelated to antigens to diphtheria and tetanus vaccines could specifically enhance the body’s res...

Images