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A kind of preparation method of hyaluronic acid nano gel

A nano-gel, hyaluronic acid technology, applied in the field of pharmacy, can solve the problems of easy degradation and inactivation, limited therapeutic effect, etc., and achieves the effects of simple preparation technology, high encapsulation capacity, and good mucoadhesion.

Inactive Publication Date: 2018-05-29
QINGDAO CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing prominent problems are: 1. Basic fibroblast growth factor is easily degraded and inactivated in the oral environment; 2. With the secretion of human saliva, basic fibroblast growth factor will be quickly cleared on the ulcer surface, limiting its therapeutic effect

Method used

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  • A kind of preparation method of hyaluronic acid nano gel
  • A kind of preparation method of hyaluronic acid nano gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 30 mg of chitosan quaternary ammonium salt with a molecular weight of 40 kDa, dissolve it in 10 mL of deionized water, drop 10 mL of 2 mg / mL basic fibroblast growth factor solution under stirring, and 1 mL of 0.2% (w / v) tripolyphosphate Sodium solution, drip after 20 minutes, stir at room temperature at 200 rpm for 30 minutes, add 10 mL of sodium hyaluronate solution containing 0.3% (w / v) dropwise under stirring conditions, drip after 30 minutes, adjust with sodium hydroxide pH to pH 4.0, stirring at room temperature, stirring at 200 r / min for 5 hours, centrifuging at 15,000 r / min for 2 hours, removing the supernatant, and freeze-drying to obtain the basic fibroblast growth factor-loaded chitosan quaternary ammonium salt hyaluronic acid Acid Nanogel.

Embodiment 2

[0033]Take 30 mg of chitosan quaternary ammonium salt with a molecular weight of 90 kDa, dissolve it in 10 mL of deionized water, drop 10 mL of 2 mg / mL basic fibroblast growth factor solution under stirring, and 1 mL of 0.3% (w / v) tripolyphosphate Sodium solution, drip after 20 minutes, stir at room temperature at 200 rpm for 30 minutes, add 10 mL of sodium hyaluronate solution containing 0.4% (w / v) dropwise under stirring conditions, drip after 30 minutes, adjust with sodium hydroxide pH to pH 5.0, stirring at room temperature, stirring at 200 rpm for 5 hours, centrifuging at 15,000 rpm for 2 hours, removing the supernatant, and freeze-drying to obtain the basic fibroblast growth factor-loaded chitosan quaternary ammonium salt hyaluronic acid Acid Nanogel.

Embodiment 3

[0035] Take 30 mg of chitosan quaternary ammonium salt with a molecular weight of 150 kDa, dissolve it in 10 mL of deionized water, drop 10 mL of 2 mg / mL basic fibroblast growth factor solution under stirring, and 1 mL of 0.5% (w / v) tripolyphosphate Sodium solution, dripped in 20 minutes, stirred at 200 rpm for 30 minutes at room temperature, added 10 mL of sodium hyaluronate solution containing 0.5% (w / v) dropwise under stirring conditions, dripped after 30 minutes, adjusted with sodium hydroxide pH to pH 6.0, stirring at room temperature, stirring at 200 r / min for 5 hours, centrifuging at 15,000 r / min for 2 hours, removing the supernatant, and freeze-drying to obtain the basic fibroblast growth factor-loaded chitosan quaternary ammonium salt hyaluronic acid Acid Nanogel.

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Abstract

The invention discloses a preparation method of hyaluronic acid nanogel. The target product hyaluronic acid nanogel is synthesized from chitosan quaternary ammonium salt and sodium hyaluronate through an ion crosslinking and polymer coacervation two-step technology, the average particle size of the product is 150-230nm, and the Zeta potential of the product is -28.6 ~ -36.7mV. The hyaluronic acid nanogel prepared in the invention can be used for treating oral ulcer, can maintain the effective active concentration of a basic fibroblast growth factor in the oral ulcer position for a long time, and improves the clinic treatment effect.

Description

technical field [0001] The invention belongs to the preparation technology of nano-gel sustained-release particles in the field of pharmacy, and particularly relates to a preparation method of hyaluronic acid nano-gel. Background technique [0002] Oral ulcer is a common disease in daily life, which occurs mostly on the tip of the tongue, the abdomen of the tongue, and the buccal mucosa, accompanied by intermittent, touching tingling, which seriously affects the quality of life of patients. At present, there is still no effective method for the treatment of oral ulcers. The traditional treatment methods focus on local pain relief, but do not pay enough attention to how to speed up the healing of the ulcer surface. [0003] Basic fibroblast growth factor is a multifunctional protein bioactive substance, which has a strong repairing effect on wounds and nerve damage. The use of basic fibroblast growth factor to treat oral ulcers has been reported. The outstanding problems ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/06A61K38/18A61K47/36A61P1/02
Inventor 卢琳李绪彤刘丽伟杜国威
Owner QINGDAO CENT HOSPITAL
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