Pulse delay adaptive calibration device, method and cell analyzer

Abstract

The present invention provides a pulse delay self-adaptive calibration device, method and cell analyzer. The method includes: obtaining the working environment parameters of the cell analyzer; according to the obtained working environment parameters, providing the cell analyzer with The coarse calibration value of the pulse delay between the non-reference laser and the reference laser; the coarse calibration value of the pulse delay is superimposed on the pulse signal of each data channel of the non-reference laser. The invention accurately finds out the delay time of the multi-channel pulse signal through an adaptive calibration method, and saves hardware resources of the pulse delay circuit part. At the same time, the original waveform data without delay alignment after pulse analog-to-digital conversion can be well preserved, which can more conveniently support subsequent data analysis and comparison.

Description

technical field [0001] The invention relates to the field of cell analysis instruments, in particular to a pulse delay self-adaptive calibration device, method and cell analyzer. Background technique [0002] Flow Cytometer (Flow Cytometer) is a modern cell analysis instrument that can perform multi-parameter and rapid quantitative analysis on single cells or biological particles in a fast, linear, flow state, and sort specific groups at the same time. [0003] Such as figure 1 As shown, in the flow cytometer, the liquid unit makes the fluorescently stained cells to be tested into a suspension, and the sample to be tested is quickly and steadily injected into the flow chamber through the pressure difference of the liquid, and the sheath flow of the liquid flow The technology arranges the cells to be tested in a single row under the sheath fluid package, and sequentially passes through the detection area of ​​the flow cytometer. The system usually uses red and blue two-way ...

AI Technical Summary

Problems solved by technology

[0013] The purpose of the present invention is to provide a pulse delay adaptive calibration device, method and cell analyzer for the pulse delay fluctuations caused by various factors in the prior art, so as to better solve the pulse delay caused by different factors in the pulse recognition process. Calculation result deviation caused by delay parameter fluctuation

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