CXCL13 oncoprotein and application of targeted medicine for CXCL13 oncoprotein in tumor aspect

A technology of oncoproteins and drugs, applied in the field of medicine, can solve the problems of unclear relationship between the expression of chemokines and lung cancer

Inactive Publication Date: 2015-11-04
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, up to now, the expression of chemokines in lung cancer and its relationship with lung cancer are not clear

Method used

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  • CXCL13 oncoprotein and application of targeted medicine for CXCL13 oncoprotein in tumor aspect
  • CXCL13 oncoprotein and application of targeted medicine for CXCL13 oncoprotein in tumor aspect
  • CXCL13 oncoprotein and application of targeted medicine for CXCL13 oncoprotein in tumor aspect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Chemokines Abnormally Expressed in Cancer Tissues of Lung Cancer Patients in Highly Polluted Areas

[0041] Using RT2-PCR Array technology to screen cancer tissues and corresponding paracancerous tissues from 8 cases of lung cancer patients in highly polluted areas, and found 4 cytokines (IL-1F5, IL-1F9, MIF, SPP1) and 7 chemokines (CXCL13, CCL7, CCL20, CCL26, CXCL6, CXCL9, CXCL14) were highly expressed relative to their paracancerous tissues. 3 cytokines (IL-1α, IL-5, TNF-α) and 9 chemokines (CCL4, CCL17, CCL18, CCL21, CCL23, CXCL1, CXCL2, CXCL3, CXCL5) were underexpressed relative to their paracancerous tissues . Among them, the high expression of chemokine CXCL13 in cancer tissues was the most significant, with an average high expression of 63 times (10.84-173.65) ( figure 1 ).

Embodiment 2

[0042] Example 2 Chemokine CXCL13 is highly expressed in cancer tissues of lung cancer patients

[0043] Through real-time PCR analysis, it was found that in highly polluted areas, 63 of the 70 samples of lung cancer patients tested had high expression of CXCL13, and the high expression rate was 90%. In the control area, 71 of the 131 samples of lung cancer patients detected high expression of CXCL13, and the high expression rate was 54.2%. Although the expression of CXCL13 in cancer tissues of lung cancer patients in different regions was increased, the mRNA level of cancer tissues in highly polluted regions was significantly higher than that in control regions. In highly polluted areas, there is no significant difference in the expression of CXCL13 between smoking lung cancer patients and non-smoking patients, suggesting the high carcinogenicity of environmental pollutants. However, in the control area, CXCL13 expression (44 / 71, 62%) was much higher in smoking lung cancer...

Embodiment 3

[0044] Example 3 High expression of CXCL13 is negatively correlated with survival and prognosis of lung cancer patients

[0045] Further analysis of the survival data of lung cancer patients revealed that the expression of CXCL13 in advanced lung cancer patients (stage III and stage IV) was significantly higher than that in early stage patients (stage I and stage II). The average survival time of lung cancer patients with high expression of CXCL13 (965 days) was significantly lower than that of patients with low expression of CXCL13 (1193 days). The above shows that the high expression of CXCL13 is negatively correlated with the survival and prognosis of lung cancer patients ( image 3 ).

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Abstract

The invention discloses CXCL13 oncoprotein and an application of targeted medicine for the CXCL13 oncoprotein in the tumor aspect. A method for preparing the tumor targeted medicine includes the steps of screening antibodies capable of effectively reducing the high expression of the CXCL13 oncoprotein or neutralizing the activity of the CXCL13 oncoprotein; preparing the corresponding tumor targeted medicine through the antibodies. According to the CXCL13 oncoprotein and the application, the anti-CXCL13 monoclonal antibodies can achieve the good anti-tumor effect by suppressing a p-AKT signal channel, a p-ERK signal channel and an EMT signal channel and accordingly suppressing growth, invasion and metastasis of tumor cells.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of an oncoprotein CXCL13 (chemokine (C-X-C motif) ligand 13) and its targeted drug in the treatment of tumors and related diseases. Background technique [0002] Lung cancer has become the malignant tumor with the highest morbidity and mortality in the world. According to different histological types, lung cancer is mainly divided into small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC). The pathogenesis of lung cancer is closely related to abnormal function of key genes, abnormal activation of signaling pathways, and tumor microenvironment. Risk factors for lung cancer mainly include exposure to radon, asbestos, genetic factors, environmental exposure, and smoking. Smoking and passive smoking are the main factors leading to lung cancer. About 87% of lung cancers are attributable to tobacco exposure. However, in recent decades, with the rapid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/395A61P35/00G01N33/574G01N33/577
Inventor 周光飚程昕王桂珍曲丽威
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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