Nickel-alhumin chelate and preparation method and application thereof

A technology of albumin and chelate, applied in the field of detection, can solve problems such as nickel pollution, and achieve the effect of improving repeatability and accuracy

Active Publication Date: 2015-11-04
上海保亿仁生物医药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] For the serious problem of nickel pollution, the object of the present invention is to provide a nickel-albumin chelate and a preparation method thereof, and to establish a qualitative and quantitative detection method for the nickel-albumin chelate, so as to quantitatively detect nickel-albumin Application of Chelate Compounds in Evaluation of Nickel Pollution Degree in a Region

Method used

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  • Nickel-alhumin chelate and preparation method and application thereof
  • Nickel-alhumin chelate and preparation method and application thereof
  • Nickel-alhumin chelate and preparation method and application thereof

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preparation example Construction

[0061] The present invention also provides a kind of preparation method of nickel-albumin chelate, comprising the following steps:

[0062] A) chelation reaction of nickel and albumin: adding nickel ions to human albumin for chelation reaction to obtain a reaction solution;

[0063] B) Extraction of purified nickel-albumin chelate: Immunoaffinity chromatography is used to remove unreacted albumin and excess nickel ions in the reaction solution to obtain nickel-albumin chelate. The specific steps are as follows:

[0064] (1) Dissolving the sample: adding physiological saline to the reaction solution obtained through step A) to redissolve the nickel-albumin chelate;

[0065] (2) Equilibrium chromatography column: use the dilution buffer to flush the pipeline of the chromatography column, fill the chromatography column with a filler that can specifically bind to albumin, and continue to use the dilution buffer to balance the chromatography column after packing the column ; The f...

Embodiment 1

[0147] Example 1 : the preparation method of nickel-albumin chelate, comprises the following steps:

[0148] A) chelation reaction of nickel and albumin: adding nickel ions to human albumin for chelation reaction to obtain a reaction solution;

[0149] Reagent preparation:

[0150] 1) Borate buffer (0.01M): Weigh 0.31g of boric acid and dissolve it in 400ml of ultrapure water, adjust the pH to 9.0 with 0.1M NaOH, and dilute to 500mL.

[0151] 2) Albumin solution: Weigh 4.0mg albumin and dissolve it in 4.0mL 0.01M pH9.0 borate buffer solution, fully shake and dissolve, and prepare a 1.0mg / mL protein solution;

[0152] 3) EDTA-NaHCO3 mixed solution: weigh EDTA·2H 2 O 1.86g, NaHCO 3 Dissolve 16.8g in 900mL ultrapure water, adjust the pH to 8.0 with 1.0M NaOH and dilute to 1000ml, autoclave, and store at room temperature;

[0153] 4) ITCBE was purchased from Japan Tongren Chemical Research Institute, the article number is M030;

[0154] 5) The dialysis bag was purchased fro...

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Abstract

The invention discloses a nickel-alhumin chelate and its preparation method and application. The nickel-alhumin chelate is formed by chelating nickel ion and alhumin through a mercapto group or/and cysteine residues. According to the invention, a qualitative and quantitative detection method of the nickel-alhumin chelate is established so as to quantitatively detect an application of the nickel-alhumin chelate in evaluating the nickel pollution level of a region. By quantitative detection of the nickel-alhumin chelate in human serum of a region, nickel pollution condition of people in the region can be reflected indirectly so as to indirectly reflect the nickel pollution level of the region. Accuracy of the nickel-alhumin chelate quantitative detection method established in the invention is greatly raised, and detection repeatability is greatly enhanced.

Description

technical field [0001] The invention relates to the detection field, in particular to a nickel-albumin chelate and its preparation method and application. Background technique [0002] Serum albumin (HSA) is the most abundant protein in mammalian plasma. It can store and transport many endogenous and exogenous substances, which is closely related to its specific structure. Serum albumin is a simple protein synthesized by the liver, consisting only of amino acids, without modifying groups and other appendages. According to the data, the bovine serum albumin (BSA) molecule consists of 583 amino acid residues. Human serum albumin (HSA) has 2 more amino acids than BSA, namely 585 amino acid residues. The difference between the two lies in the deletion of residues 116 and 585 in the amino acid sequence of HSA in BSA. The most notable feature of the amino acid composition of albumin is the low content of tryptophan, with HSA and BSA containing 1 or 2 residues per molecule, resp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/53G01N27/62G01N1/28G01N27/447G01N27/626
Inventor 张积仁阳帆董欣敏吴婧蔡睿孙遥赵乙木
Owner 上海保亿仁生物医药有限公司
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