A method for cultivating saline biofilm

A technology for salt-adaptive microorganisms and biofilms is applied in the field of cultivation of salt-adapted biofilms in the biochemical treatment of high-salt organic wastewater, and can solve the problems of poor adaptability, insufficient salt tolerance and low stability of high-salt wastewater. , to achieve the effect of short implementation period, good organic degradation ability, and strong resistance to salinity shock

Active Publication Date: 2017-11-14
BLUESTAR LEHIGH ENG INST CO LTD
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AI Technical Summary

Problems solved by technology

[0004] The problem to be solved by the present invention is that the biofilm method has defects such as insufficient salt tolerance, poor adaptability, and low stability in the treatment of high-salt wastewater. Method for cultivating salt-suitable biofilm required for biochemical treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, a method for cultivating a saline-suitable biofilm, the method is to use a high-salt environment sample as a saline-suitable microorganism The source of bacterial flora, firstly carry out mixed enrichment culture and then carry out two stages of film-hanging culture; enrichment culture is based on high-salt environment samples as the source of salt-suitable microorganisms, and carry out mixed enrichment culture of the salt-suitable microbial flora; The culture is to add the mixed enrichment culture solution of salt-suitable microorganisms into the aerobic reactor equipped with fillers or carriers, and continue the mixed culture of the salt-suitable microbial flora. During the cultivation process, the dissolved oxygen is first controlled at 0.5mg / L, and then gradually increased to 1.0mg / L and 2.0mg / L, the salt-suitable microbial flora will gradually gather, grow and form biofilm on the surface of the filler;

[0027] The salt-suitable microorganisms refer t...

Embodiment 2

[0029] Embodiment 2, a kind of method for cultivating saline suitable biofilm described in embodiment 1, this method is under the condition of inorganic salt concentration 3%~7%, pH6.5~8.0, temperature 15~25 ℃, with high salt Environmental samples are used as the source of salt-suitable microbial flora. Firstly, two stages of mixed enrichment culture and then film-hanging culture are carried out; during the culture process, the dissolved oxygen is first controlled at 0.8mg / L, and then gradually increased to 1.5mg / L and 2.5mg / L, all the other are identical with embodiment 1.

Embodiment 3

[0030] Embodiment 3, a kind of method for cultivating saline biofilm described in embodiment 1, this method is under the condition of inorganic salt concentration 15%~25%, pH8.0~9.0, temperature 30~40 ℃, with high salt Environmental samples are used as the source of salt-suitable microbial flora. First, mixed enrichment culture is carried out and then two stages of film-hanging culture are carried out; during the culture process, dissolved oxygen is first controlled at 1.0mg / L, and then gradually increased to 2.0mg / L and 4.0mg / L, all the other are identical with embodiment 1.

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Abstract

The invention relates to a method for cultivating saline-suitable biofilms. The method is to use high-salt environment samples as salt-suitable microbial flora under the conditions of salt concentration 1%-25%, pH 6.0-9.0, and temperature 10-40°C. source, first carry out mixed enrichment culture and then carry out film-hanging culture; in the process of film-hanging culture, first control dissolved oxygen 0.5~1.0mg / L, and then gradually increase to 1.0~2.0mg / L and 2.0~4.0mg / L, so that Halo-suitable microbial flora aggregates, attaches and grows on the surface of fillers or carriers to form biofilms. By adopting the method of the invention, a salt-suitable biofilm can be cultivated in a salt environment of 1% to 25%, and pollutants in waste water can be efficiently degraded under the condition of a salt concentration of 1% to 25%.

Description

technical field [0001] The invention belongs to the technical field of biochemical treatment of high-salt wastewater, and in particular relates to a method for cultivating a salt-suitable biofilm in a biofilm process for biochemical treatment of high-salt organic wastewater. Background technique [0002] High-salt wastewater refers to wastewater with a mass fraction of inorganic salts greater than 1%. Its sources include: (1) seawater utilization: wastewater generated from seawater processing, mariculture, and seawater substitute discharge; (2) industrial wastewater: food, petroleum, chemical industry , printing and dyeing, papermaking, leather, pharmaceutical, fermentation and other industries widely produce high-salt and high-concentration organic wastewater; (3) Others: ship ballast water, salt water generated by minimizing waste water (garbage leachate, reverse osmosis concentrated drainage), Domestic sewage from large ships. The high-salt wastewater has a large amount ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34
Inventor 王开春徐军李坤赵焰王克云袁丽娟刘娟崔庆兰
Owner BLUESTAR LEHIGH ENG INST CO LTD
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