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A method for preparing egg yolk lecithin for injection by static adsorption of alumina to remove impurities and low-temperature precipitation for deoiling

A technology of egg yolk lecithin and static adsorption, applied in the directions of edible phospholipid composition, application, protein food ingredients, etc., can solve the problems of difficult removal of impurities, difficult recovery and reuse of degreasing solvent, large amount of degreasing solvent, etc. The effect of operation, low cost and low solvent consumption

Active Publication Date: 2017-04-19
SHENYANG TIANFENG BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the above deficiencies in the prior art, the present invention provides a method for preparing egg yolk lecithin for injection by static adsorption of alumina to remove impurities and low-temperature precipitation deoiling, which solves the difficulty of removing impurities such as LPE and PI in the lecithin refining process , The amount of deoiling solvent is large, and the deoiling solvent is not easy to recycle and reuse.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 600g of egg yolk powder and place it in a 5000ml Erlenmeyer flask, add 2400ml of absolute ethanol, stir and leach for 2 hours when the liquid temperature is 40°C, filter cloth; add 1800ml of absolute ethanol to the filter cake, Stir and extract at 40°C for 2 hours, filter the cloth, combine the two extracts, concentrate under reduced pressure at 50°C to obtain a concentrated solution, let stand until complete separation, and remove the lower oil phase.

[0022] Add 48g of 200-300 mesh neutral alumina to the concentrated solution, raise the temperature of the drug solution to 25°C, stir and adsorb for 1 hour, pass through a 0.45 μm filter membrane, concentrate the filtrate under reduced pressure at 50°C, and dry it in vacuum, which is the ethanol extraction dry product. The mass is 230g.

[0023] Add 2300ml of mixed solvent of n-hexane: acetone = 1:5 to the ethanol extraction dry product, heat to 35°C under stirring condition to dissolve, continue stirring at this t...

Embodiment 2

[0026] Get 600g egg yolk powder and place in 5000ml Erlenmeyer flask, add 3000ml 95% ethanol, when liquid temperature is 25 ℃, stir and extract 50min, filter cloth; Stir and leach for 50min at 25°C, filter cloth; add 1800ml 95% ethanol to the filter cake, stir and leach for 50min when the liquid temperature is 25°C, filter cloth; add 1800ml 95% ethanol to the filter cake, Stir and extract for 50 minutes when the liquid temperature is 25°C, filter cloth. The four extracts were combined and concentrated under reduced pressure at 50°C to obtain a concentrated solution, which was allowed to stand until the layers were completely separated, and the lower oil phase was removed.

[0027] Add 60g of 200-300 mesh neutral alumina to the concentrated solution, raise the temperature of the drug solution to 40°C, stir and absorb for 3 hours, pass through a 0.45 μm filter membrane, concentrate the filtrate under reduced pressure at 50°C, and dry it in a vacuum, which is the ethanol extracti...

Embodiment 3

[0031]Get 600g egg yolk powder and place it in a 5000ml Erlenmeyer flask, add 2400ml 99% ethanol, stir and extract 90min when the liquid temperature is 30°C, filter cloth; add 1800ml 99% ethanol again in the filter cake, Stir and extract for 90 minutes at 30°C, filter cloth; add 1800ml of 99% ethanol to the filter cake, stir and extract for 90 minutes when the liquid temperature is 30°C, filter cloth. The three extracts were combined and concentrated under reduced pressure at 50°C to obtain a concentrated solution, which was allowed to stand until the layers were completely separated, and the lower oil phase was removed.

[0032] Add 54g of 60-180 mesh neutral alumina to the concentrated solution, raise the temperature of the liquid to 33°C, stir and adsorb for 1.5h, pass through a 0.22μm filter membrane, concentrate the filtrate under reduced pressure at 50°C, and dry it in vacuum, which is the ethanol extraction dry matter , with a mass of 234g.

[0033] Add 2574ml of mixed...

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Abstract

The invention discloses a method for preparing egg yolk lecithin for injection through static adsorption of alumina to remove impurities and low-temperature precipitation and deoiling, and belongs to the technical field of egg yolk lecithin refining. The method includes the following steps: using egg yolk powder or egg yolk granules as raw materials, extracting with absolute ethanol, statically adsorbing aluminum oxide, dissolving with a mixed solution of n-hexane-acetone, precipitating lecithin at low temperature, and drying the precipitate to obtain the product. The obtained product was tested by thin-layer chromatography, and the residues of triglyceride, cholesterol and palmitic acid met the limit requirements of the Chinese Pharmacopoeia 2015 edition. The invention provides a brand-new process method for removing impurities and deoiling egg yolk lecithin, and the method has high yield and good effect of removing impurities and deoiling. Alumina static adsorption takes short working hours and is easy to operate, and can remove impurities such as LPE and PI smoothly; and the equipment is simple, the investment in fixed assets is small, the solvent is easy to recycle and reuse, and the discharge of three wastes is greatly reduced.

Description

technical field [0001] The invention relates to the technical fields of separation and purification and lecithin, in particular to a method for preparing egg yolk lecithin for injection by static adsorption of alumina and deoiling by low-temperature precipitation. Background technique [0002] Phospholipids are ubiquitous in various tissues and organs of the human body, especially in the brain, nervous system, heart, lung, kidney, spleen, blood, and emulsion, and are components of biofilms. Egg yolk lecithin for injection has high nutritional value and physiological activity, and has significant curative effects in protecting cell membranes, anti-aging, lowering blood fat, and preventing and treating fatty liver. In addition to biological functions and pharmaceutical applications, phospholipids are widely used in food industry, light industry, chemical industry and other fields, because phospholipids also have a variety of functional characteristics, such as emulsification, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/10
Inventor 王志强王继文李津鞠加林
Owner SHENYANG TIANFENG BIOLOGICAL PHARMA
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