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Preparation method and application of nano chitosan

A nano-chitosan, chitosan technology, applied in medical science, prosthesis and other directions, can solve the problems of death, affecting the skin barrier function and appearance of patients, and achieve the effect of delaying healing, promoting wound healing, and promoting skin regeneration.

Active Publication Date: 2015-11-11
SHANDONG ANTE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ulcers caused by burns, varicose veins, vasculitis, skin cancer, etc., and skin defects caused by acute trauma seriously affect the skin barrier function and appearance of patients, and can lead to death in severe cases.

Method used

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  • Preparation method and application of nano chitosan
  • Preparation method and application of nano chitosan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: a kind of preparation method of nano chitosan is as follows:

[0025] (1) Purification of chitosan: Add 200ml of deionized water, 4ml of glacial acetic acid, and 8g of chitosan into a round bottom flask, heat and stir in a water bath, the temperature of the water bath is 30°C, and the stirring speed is 1000r / min; carry out after 1h Suction filtration, adding sodium hydroxide solution dropwise to the obtained filtrate to adjust the pH value of the filtrate to 8 to precipitate chitosan; let stand for 3h, collect the chitosan precipitate, and wash the chitosan precipitate with deionized water until the pH is 7 , remove moisture and dry to obtain purified chitosan;

[0026] (2) Preparation of chitosan emulsion: Add 100ml of deionized water and 2ml of glacial acetic acid into the beaker, heat and stir in a water bath, the speed is 1000r / min, the temperature is 30°C, take 5g of purified chitosan, slowly add In the acetic acid solution; keep the rotational speed...

Embodiment 2

[0029] Embodiment 2: a kind of application method of nano chitosan in artificial skin is as follows:

[0030] (1) Preparation of nano-chitosan solution: add 66.15ml of deionized water and 1.4ml of glacial acetic acid into the beaker, stir evenly, add 2.45g of nano-chitosan, and ultrasonically dissolve at 30°C to obtain a nano-chitosan solution;

[0031] (2) Preparation of gelatin solution: Add 2g of gelatin and 25ml of deionized water into a round bottom flask, heat and stir in a water bath, the temperature of the water bath is 40°C, the stirring speed is 800r / min, and the gelatin solution is obtained after 1h;

[0032](3) Solution mixing: adjust the temperature of the water bath to 30°C and the stirring speed to 800r / min, add the chitosan solution prepared in step (1) to the gelatin solution prepared in step (2), and keep the temperature of the water bath at 30°C , stirring speed 800r / min, obtain chitosan-gelatin solution after 1h;

[0033] (4) Preparation of artificial skin...

Embodiment 3

[0035] Embodiment 3: a kind of application method of nano chitosan in artificial skin is as follows:

[0036] (1) Preparation of nano-chitosan solution: add 66.15ml of deionized water and 1.4ml of glacial acetic acid into the beaker, stir evenly, add 2.45g of nano-chitosan, and ultrasonically dissolve at 30°C to obtain a nano-chitosan solution;

[0037] (2) Preparation of gelatin solution: Add 5g of gelatin and 30ml of deionized water into a round bottom flask, heat and stir in a water bath, the temperature of the water bath is 60°C, and the stirring speed is 1200r / min, and the gelatin solution is obtained after 1h;

[0038] (3) Solution mixing: adjust the water bath temperature to 40°C and the stirring speed to 1200r / min, add the chitosan solution prepared in step (1) to the gelatin solution prepared in step (2), and keep the water bath temperature at 40°C , stirring speed 1200r / min, obtain chitosan-gelatin solution after 1h;

[0039] (4) Preparation of artificial skin: Add ...

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Abstract

Preparation of nano chitosan in the invention almost has no chemical reaction, chitosan is mainly dissolved and dispersed, and chitosan is not dissolved in a sodium hydroxide solution, so that chitosan particles are separated out. Meanwhile, preparation conditions are mild, the safety is high, and preparation raw materials are common and nontoxic. The obtained nano chitosan is an ideal raw material for preparing artificial skin, the prepared artificial skin is soft in texture and is comfortable, the laminating performance between the prepared artificial skin and a wound surface is good, and the prepared artificial skin is breathable, can absorb water, has antibacterial and anti-inflammatory effects, and has the functions of stopping pain, arresting bleeding and promoting wound healing. Along with the healing of the wound of a patient and the growth of the skin of the patient, the chitosan artificial skin can be automatically dissolved and absorbed by the body, scraps cannot be left so as to avoid the delay of wound healing, and skin regeneration can be promoted. Moreover, the operation is simple, the safety is improved, and preparation processes are simplified. Required instruments are relatively common so as to be favorable for reducing the cost, and thus the nano chitosan has important application value in the field of artificial skin.

Description

technical field [0001] The invention relates to a method for preparing nano-chitosan and its application, in particular to a method for preparing nano-chitosan and the application of the prepared nano-chitosan in artificial skin. Background technique [0002] Chitosan is the only basic polysaccharide among natural polysaccharides, which has good biocompatibility and biodegradability, and has various biological activities. Chitosan is the second largest natural bio-organic resource next to cellulose in nature. It is obtained by deacetylating chitin. Chitin is a polysaccharide biopolymer widely distributed in nature, mainly in lower biological fungi, arthropod shrimp and crab and other insect shells and cells of higher plants can be said to be everywhere. The annual synthesis of chitin reaches 200 billion tons, and it is an inexhaustible biological resource. [0003] The skin is the largest organ of the human body. It has important functions such as resisting external microb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C08J3/03C08J3/12C08J5/18C08L5/08C08L89/00A61L27/60
Inventor 谢宇王健鹏
Owner SHANDONG ANTE BIOTECH CO LTD
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