Pelvic cavity prolapse high expression gene and application thereof

A pelvic prolapse and genetic technology, applied in the field of biomedicine, can solve the problems of inability to fully elucidate the mechanism of pelvic floor support tissue damage, unknown etiology and pathogenesis, and unclear pathogenesis, achieving good application prospects and simplifying quantitative detection Clear process and results

Active Publication Date: 2015-11-11
GUAN BOJIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few basic studies on POP, and the fields involved are limited. Its etiology and pathogenesis are unknown, especially the basic research at the molecular level cannot fully clarify the damage mechanism of the pelvic floor support tissue, and more in-depth and extensive discussions are needed.
Because its pathogenesis is still unclear, most of the current treatments only treat the symptoms, but cannot cure the disease from the cause

Method used

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  • Pelvic cavity prolapse high expression gene and application thereof
  • Pelvic cavity prolapse high expression gene and application thereof
  • Pelvic cavity prolapse high expression gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The collection of embodiment 1 case

[0035] From September 2012 to December 2013, a total of 9 POP patients who visited the Department of Obstetrics and Gynecology of Union Medical College Hospital were collected. A total of 3 cases were collected using age and place of residence as matching factors. The uterosacral ligament tissue samples of all subjects were obtained, numbered and stored in a -80°C low-temperature refrigerator.

[0036] Inclusion criteria for the POP group: a. POP-Q score ≥ II degree after gynecological examination;

[0037] b. Continuously living in the Beijing area;

[0038] c. At least one childbirth history.

[0039] Exclusion criteria: a. Patients who received hormone replacement therapy in the past 3 months;

[0040] b. Patients with connective tissue diseases (rheumatoid arthritis, systemic lupus erythematosus, polymyositis, Marfan syndrome, Eydown syndrome);

[0041] c. Pregnant patients;

[0042] d. The patient refuses to join;

[004...

Embodiment 2

[0055] Example 2 High-throughput sequencing and analysis

[0056] RNA extraction is performed on the tissue, followed by agarose gel electrophoresis after RNA extraction. From the results of electrophoresis, it can be preliminarily judged whether the quality of the extracted RNA sample is qualified or not, and whether it can be used for further transcriptome analysis. Then, the extraction of RNA samples was detected by NanoDrop1000 spectrophotometer, and the sample requirements for RNA-seq sequencing: OD260 / OD280 was 1.8-2.2.

[0057] The sequencing platform is the HiSeq2500 high-throughput sequencing platform of Illumina, which performs high-throughput transcriptome deep sequencing. After sequencing, we use Fast-QC (http: / / www.bioinformatics.babraham.ac.uk / projects / fastqc / ) software Overall assessment of the quality of sequencing data, including base quality value distribution, quality value position distribution, GC content, PCRduplication content, kmer frequency, etc. In t...

Embodiment 3

[0058] Example 3 CHRDL2 gene expression in pelvic prolapse patients and controls in uterosacral ligament tissue

[0059] 1. Materials and methods

[0060] 1. Materials

[0061] The uterosacral ligament tissues of 35 cases of pelvic prolapse patients and 5 cases of control uterosacral ligament tissues were selected and grouped and numbered. The control group was patients with benign gynecological diseases undergoing laparotomy or vaginal hysterectomy, pelvic organ prolapse was ruled out by vaginal examination, and no urinary incontinence symptoms. Of the 35 patients with pelvic prolapse, 17 were grade II, 8 were grade III, and 10 were grade IV.

[0062] 2. Method

[0063] 2.1 Extraction of total RNA from uterosacral ligament tissues of patients with pelvic prolapse and controls

[0064] use Reagent (invitrogen, Cat. No. 15596-018) was used to extract sample RNA, and the experimental operation was carried out according to the product manual. The specific operation was as f...

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Abstract

The invention relates to a pelvic cavity prolapse high expression gene and application thereof, in particular to application of genes CHRDL2 in diagnosis and treatment of pelvic cavity prolapse. Based on a high-throughput sequencing result, the inventor selects candidate genes CHRDL2, a fluorescent quantitation result proves that CHRDL2 is obviously high in expression in pelvic cavity prolapse, and a cell biology experiment further shows that the genes CHRDL2 affect growth and proliferation activity of uterosacral ligament fibroblasts. The genes CHRDL2 can be used for auxiliary diagnosis and treatment on pelvic cavity prolapse, and have important clinical application value.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a highly expressed gene of pelvic cavity prolapse and its application, more specifically to the application of CHRDL2 gene in the diagnosis and treatment of pelvic cavity prolapse. Background technique [0002] Pelvic organ prolapse (Pelvic organ prolapse, POP) is caused by the defect, damage or dysfunction of the pelvic floor support structure. It is a common disease that affects the quality of life of middle-aged and elderly women. caused by obstacles. There are few basic studies on POP, and the fields involved are limited. Its etiology and pathogenesis are unknown, especially the basic research at the molecular level has not yet fully elucidated the damage mechanism of the pelvic floor support tissue, and more in-depth and extensive discussions are needed. Because its pathogenesis is still unclear, most of the current treatments only treat the symptoms, but cannot cure the disease ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A61K45/00A61K31/7088A61P15/00G01N33/68G01N33/577
CPCA61K31/7088A61K45/00C12Q1/6883C12Q2600/158G01N33/6893G01N2333/47G01N2800/36
Inventor 孙锦云杨承刚
Owner GUAN BOJIAN BIOTECH CO LTD
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