Liquid band-aid and preparing method thereof

Abstract

The invention discloses a liquid band-aid, comprising, active materials, a film-forming material and a solvent. The active materials include cytokines 0.042 to 0.0095%, which are chosen from at least two of EGF (epidermal growth factor), PDGF (platelet derived growth factor), FGF (fibroblast growth factor) and IL-10 (interleukin-10). The film-forming material comprises chitosan hydrochloride 2% to 8%. The solvent is deionized water or distilled water. The invention further discloses a method for preparing the liquid band-aid. By the use of the preparation method and / or the liquid band-aid comprising various biological active materials and prepared by the preparation method, activity of the biological active materials can be retained, and wounds can be efficiently repaired without a scar.

Description

technical field

[0001] The invention relates to the field of medical dressings, specifically, the invention relates to a liquid dressing, and more specifically, the invention relates to a liquid band-aid and a preparation method thereof. Background technique

[0002] Wound repair is a complex process involving multi-cells and multi-cytokines. Wound dressings play an important role in treating or repairing wounds. When treating wounds with dressings, the main purpose is to disinfect and sterilize them to avoid further infection of the wound. Then use the body's own healing and repairing ability to wait for the wound to heal. At present, the finished wound repair dressings on the market are in patch or powder form, which are extremely inconvenient for large-area, irregular or difficult-to-treat wounds.

[0003] Liquid Band-Aid is a new type of Band-Aid that has emerged in recent years and is different from traditional Band-Aids. Traditional Band-Aids are strips of adhesive ta...

Examples

Embodiment 1

[0026] 1. Raw material composition and ratio

[0027] Active substances: EGF, PDGF, FGF, IL-10, and vesicles, of which EGF is 0.002%, PDGF is 0.0005%, FGF is 0.005%, IL-10 is 0.0005%, and vesicles are 14%;

[0028] The solvent is deionized water; the film-forming components include: chitosan hydrochloride accounts for 6%, and polyvinylpyrrolidone accounts for 2%;

[0029] Other components: silver ion 0.05ppm, hyaluronic acid 0.05%, chondroitin sulfate: 5%.

[0030] 2. Preparation method

[0031] (1) Preparation of vesicles

[0032] Collect the serum-free medium of mesenchymal stem cells with low passage number (less than 10 passages) and grow within 48 hours, centrifuge at 500g for 30 minutes at 4°C, and collect the centrifuged supernatant;

[0033] Remove impurities by filtration with 0.22 μm filter membrane, centrifuge at 100,000 g for 70 minutes at 4°C, and remove the supernatant;

[0034] Resuspend the pellet, wash the pellet with PBS, centrifuge at 100,000g at 4°C for...

Embodiment 2

[0043] 1. Raw material composition and ratio

[0044] Active substances: EGF, PDGF, FGF, of which EGF is 0.003%, PDGF is 0.0005%, and FGF is 0.005%;

[0045] The solvent is deionized water;

[0046] The film-forming components include: chitosan hydrochloride accounts for 8%, polyvinylpyrrolidone accounts for 4%;

[0047] Other components: silver ion 0.05ppm, hyaluronic acid 0.1%, chondroitin sulfate: 4%.

[0048] 2. Preparation method

[0049] (1) 8g chitosan hydrochloride is dissolved in 50ml deionized water to obtain chitosan hydrochloride aqueous solution;

[0050] (2) 4g of polyvinylpyrrolidone is dissolved in 20ml of deionized water to obtain an aqueous solution of polyvinylpyrrolidone;

[0051] (3) Mix (1) and (2), mechanically stir and mix at 60°C and 1000rpm. Slow down the stirring speed to 400rpm, reduce and keep the temperature to control the temperature of the mixed solution not to be higher than 37°C. During the stirring process, add 4g chondroitin sulfate, 0....

Embodiment 3

[0055] 1. Raw material composition and ratio

[0056] Active substances: EGF, PDGF, FGF, IL-10, vesicles, of which EGF is 0.003%, PDGF is 0.0005%, FGF is 0.002%, IL-10 is 0.001%, and vesicles are 14.5%;

[0057] The solvent is deionized water;

[0058] Film-forming components: chitosan hydrochloride accounted for 6%, polyvinylpyrrolidone accounted for 0.5%;

[0059] Other components: silver ion 0.05ppm, hyaluronic acid 0.1%, chondroitin sulfate 2%;

[0060] 2. Preparation method

[0061] (1) 6g chitosan hydrochloride is dissolved in 50ml deionized water to obtain chitosan hydrochloride aqueous solution; 0.05g polyvinylpyrrolidone is dissolved in 20ml deionized water to obtain polyvinylpyrrolidone aqueous solution;

[0062] (2) Mix the above two solutions, keep the temperature at 45-60° C., and mechanically stir at 2000 rpm. Reduce the stirring speed to 500rpm, control the temperature of the mixture at 37°C, add 2g chondroitin sulfate, 0.1g hyaluronic acid, 3mgEGF, 0.5mgPDG...

technical field

[0001] The invention relates to the field of medical dressings, specifically, the invention relates to a liquid dressing, and more specifically, the invention relates to a liquid band-aid and a preparation method thereof. Background technique

[0002] Wound repair is a complex process involving multi-cells and multi-cytokines. Wound dressings play an important role in treating or repairing wounds. When treating wounds with dressings, the main purpose is to disinfect and sterilize them to avoid further infection of the wound. Then use the body's own healing and repairing ability to wait for the wound to heal. At present, the finished wound repair dressings on the market are in patch or powder form, which are extremely inconvenient for large-area, irregular or difficult-to-treat wounds.

[0003] Liquid Band-Aid is a new type of Band-Aid that has emerged in recent years and is different from traditional Band-Aids. Traditional Band-Aids are strips of adhesive ta...

Examples

Embodiment 1

[0026] 1. Raw material composition and ratio

[0027] Active substances: EGF, PDGF, FGF, IL-10, and vesicles, of which EGF is 0.002%, PDGF is 0.0005%, FGF is 0.005%, IL-10 is 0.0005%, and vesicles are 14%;

[0028] The solvent is deionized water; the film-forming components include: chitosan hydrochloride accounts for 6%, and polyvinylpyrrolidone accounts for 2%;

[0029] Other components: silver ion 0.05ppm, hyaluronic acid 0.05%, chondroitin sulfate: 5%.

[0030] 2. Preparation method

[0031] (1) Preparation of vesicles

[0032] Collect the serum-free medium of mesenchymal stem cells with low passage number (less than 10 passages) and grow within 48 hours, centrifuge at 500g for 30 minutes at 4°C, and collect the centrifuged supernatant;

[0033] Remove impurities by filtration with 0.22 μm filter membrane, centrifuge at 100,000 g for 70 minutes at 4°C, and remove the supernatant;

[0034] Resuspend the pellet, wash the pellet with PBS, centrifuge at 100,000g at 4°C for...

Embodiment 2

[0043] 1. Raw material composition and ratio

[0044] Active substances: EGF, PDGF, FGF, of which EGF is 0.003%, PDGF is 0.0005%, and FGF is 0.005%;

[0045] The solvent is deionized water;

[0046] The film-forming components include: chitosan hydrochloride accounts for 8%, polyvinylpyrrolidone accounts for 4%;

[0047] Other components: silver ion 0.05ppm, hyaluronic acid 0.1%, chondroitin sulfate: 4%.

[0048] 2. Preparation method

[0049] (1) 8g chitosan hydrochloride is dissolved in 50ml deionized water to obtain chitosan hydrochloride aqueous solution;

[0050] (2) 4g of polyvinylpyrrolidone is dissolved in 20ml of deionized water to obtain an aqueous solution of polyvinylpyrrolidone;

[0051] (3) Mix (1) and (2), mechanically stir and mix at 60°C and 1000rpm. Slow down the stirring speed to 400rpm, reduce and keep the temperature to control the temperature of the mixed solution not to be higher than 37°C. During the stirring process, add 4g chondroitin sulfate, 0....

Embodiment 3

[0055] 1. Raw material composition and ratio

[0056] Active substances: EGF, PDGF, FGF, IL-10, vesicles, of which EGF is 0.003%, PDGF is 0.0005%, FGF is 0.002%, IL-10 is 0.001%, and vesicles are 14.5%;

[0057] The solvent is deionized water;

[0058] Film-forming components: chitosan hydrochloride accounted for 6%, polyvinylpyrrolidone accounted for 0.5%;

[0059] Other components: silver ion 0.05ppm, hyaluronic acid 0.1%, chondroitin sulfate 2%;

[0060] 2. Preparation method

[0061] (1) 6g chitosan hydrochloride is dissolved in 50ml deionized water to obtain chitosan hydrochloride aqueous solution; 0.05g polyvinylpyrrolidone is dissolved in 20ml deionized water to obtain polyvinylpyrrolidone aqueous solution;

[0062] (2) Mix the above two solutions, keep the temperature at 45-60° C., and mechanically stir at 2000 rpm. Reduce the stirring speed to 500rpm, control the temperature of the mixture at 37°C, add 2g chondroitin sulfate, 0.1g hyaluronic acid, 3mgEGF, 0.5mgPDG...