Chicken' semen diluent and application thereof
A technology of diluent and semen, applied in applications, medical preparations containing active ingredients, antibacterial drugs, etc., can solve the problem of lax disinfection of insemination instruments, decline of egg fertilization rate and egg production rate, increase of dead scouring, etc. problems, to achieve the effect of less rooster dosage, lasting and stable fertilization rate, and saving the cost of feed use
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Embodiment 1
[0024] Under sterile conditions, add 4g of glucose and 1.5ml of egg yolk to 100ml of distilled water to dissolve, then add 1g of cephalosporin, 1g of amikacin and 1g of ribavirin, mix well to obtain chicken semen dilution, and adjust the pH to 6.9 ~7.0, osmotic pressure is 330~360mOsm, buffer index is 5.5~461, low temperature preservation.
Embodiment 2
[0026] Chicken artificial insemination: During use, the chicken semen diluent is preheated to 37°C for later use, and semen is collected by back-abdominal combined massage method and diluted 1:1 with the wet semen diluent on the spot, and the diluted semen is diluted within 0.5h Lost to the hen. The hen is inseminated with the method of turning the anus, and each hen is inseminated once every 5 days, and the diluted semen is 30-50 microliters each time. The eggs were collected 48 hours after insemination, and the eggs were hatched within 1 week after insemination, and the fertilization rate was measured on the 7th day of incubation.
Embodiment 3
[0028] Chicken artificial insemination: During use, the chicken semen diluent is preheated to 37°C for later use, and the semen is collected by combined dorsal and abdominal massage method, and is diluted 2:1 with the wet semen diluent on the spot, and the diluted semen will be diluted within 0.5h Lost to the hen. The hen is inseminated with the method of turning the anus, and each hen is inseminated once every 5 days, and the diluted semen is 30-50 microliters each time. The eggs were collected 48 hours after insemination, and the eggs were hatched within 1 week after insemination, and the fertilization rate was measured on the 7th day of incubation.
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