A kind of detection method of gizzard erosin in fish meal
A technology for the detection of gizmosin and its detection method, which is applied in the field of high performance liquid chromatography detection of gizmosin in fishmeal, which can solve the problems of separation interference and complex components of fishmeal, and achieve high accuracy, high sensitivity, and separation practicability high effect
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Embodiment 1
[0036] A detection method for gizzard erosin in fishmeal, comprising the following steps:
[0037] (1) Sample pretreatment
[0038] Take 100 mg of fish meal and add it to the hydrolysis tube, then add 5 mL of 6 mol / L hydrochloric acid, screw the cap tightly, put it in a drying oven at 109 °C, hydrolyze for 22 h, filter to obtain the hydrolyzate, and then oxidize it with 10 mol / L hydroxide The sodium solution adjusts the pH value to 6.8, and after standing still, centrifuges and takes the supernatant to obtain a crude extract;
[0039] (2) Enrichment and purification
[0040] Take 3 mL of methanol and 3 mL of ultrapure water to activate the MCX solid-phase extraction column, inject the crude extract obtained in step (1) into the MCX solid-phase extraction column for enrichment, and then rinse the MCX solid-phase extraction column with 3 mL of water and 3 mL of methanol, respectively. The phase extraction column was drained and eluted with 3 mL of ammonia-methanol solution, an...
Embodiment 2
[0044] A detection method for gizzard erosin in fishmeal, comprising the following steps:
[0045] (1) Sample pretreatment
[0046] Take 500 mg of fish meal and add it to the hydrolysis tube, then add 10 mL of 6 mol / L hydrochloric acid, screw the cap tightly, put it in a drying oven at 111 °C, hydrolyze for 24 h, filter to obtain the hydrolyzate, and then oxidize it with 5 mol / L hydroxide The sodium solution adjusts the pH value to 6.8, and after standing still, centrifuges and takes the supernatant to obtain a crude extract;
[0047] (2) Enrichment and purification
[0048] Take 3 mL of methanol and 3 mL of ultrapure water to activate the MCX solid-phase extraction column, inject the crude extract obtained in step (1) into the MCX solid-phase extraction column for enrichment, and then rinse the MCX solid-phase extraction column with 3 mL of water and 3 mL of methanol, respectively. The phase extraction column was drained and eluted with 5 mL of ammonia-methanol solution, and ...
Embodiment 3
[0052] A detection method for gizzard erosin in fishmeal, comprising the following steps:
[0053] (1) Sample pretreatment
[0054] Take 300 mg of fish meal and add it to the hydrolysis tube, then add 8 mL of 6 mol / L hydrochloric acid, screw the cap tightly, put it in a drying oven at 110 °C, hydrolyze for 23 h, filter to obtain the hydrolyzate, and then oxidize it with 8 mol / L hydroxide Adjust the pH value to 7.00 with the sodium solution, after standing still, centrifuge and take the supernatant to obtain a crude extract;
[0055] (2) Enrichment and purification
[0056] Take 3 mL of methanol and 3 mL of ultrapure water to activate the MCX solid-phase extraction column, inject the crude extract obtained in step (1) into the MCX solid-phase extraction column for enrichment, and then wash the MCX with 3 mL of water and 3 mL of methanol respectively The solid-phase extraction column was eluted with 4 mL of ammonia-methanol solution after pumping dry, and the eluate was blown ...
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