Extraction method of gracilaria blodgettii protoplast

Abstract

The invention discloses an extraction method of gracilaria blodgettii protoplast. The technical scheme mainly comprises researching on the effects of different tool enzymes decomposing alga tissue, establishing the preparation method for gracilaria blodgettii protoplast, and obtaining of dissociated somatic cell protoplast. The advantages comprise that gracilaria blodgettii protoplast is successfully obtained, and is applicable to fields of genetic transformation, somatic cell hybridization, regeneration plant induction, somatic cell engineering seedling growing, etc.

Description

technical field [0001] The invention relates to an algae cell engineering method, in particular to a method for preparing the protoplast of Gracilaria taro root. Background technique [0002] Protoplast is the bare cell after removal of the cell wall of the plant cell. It can reproduce in large numbers and rapidly under artificially controlled conditions, and can also be induced to fuse to form hybrid cells, providing experimental materials for somatic cell hybridization. Protoplasts are generally obtained by mechanically disrupting or chemically degrading the cells to remove or dissolve the cell wall, leaving a viable part surrounded by the plasma membrane. The research on protoplasts can be traced back to 1892. Klercker put the leaves of the aquatic plant water sword leaf into a hypertonic solution for cultivation, but it was not until 1960 that a breakthrough was made in how to prepare free protoplasts with vitality. University of Nottingham Using cellulase isolated from...

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Problems solved by technology

In addition, the cell wall of Gracilaria contains thick colloids, which cannot directly perform genetic operations such as cell fusion and cell hybridization. The protoplasts obtained by conventional enzymatic dissolution methods in the prior art often have low activity an

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