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A color super-resolution imaging device and method

A technology of super-resolution imaging and super-resolution images, which is applied in the field of color super-resolution imaging devices, can solve the problems of low signal acquisition rate and efficiency, and achieve the effect of fast imaging speed

Active Publication Date: 2017-11-07
SHENZHEN INST OF ADVANCED TECH
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  • Application Information

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Problems solved by technology

[0006] Embodiments of the present invention provide a color super-resolution imaging device and method to solve the problem of low signal acquisition rate and efficiency in the current secondary scanning super-resolution microscopy technology

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  • A color super-resolution imaging device and method
  • A color super-resolution imaging device and method

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Embodiment Construction

[0042] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0043] Such as figure 2 As shown, the embodiment of the present invention provides a color super-resolution imaging device for microscopic imaging of the sample 8 to be tested. The color super-resolution imaging device includes:

[0044] Laser emitter 1 , first dichroic mirror 3 , beam splitter 13 , CCD camera 16 and three-channel photomultiplier tube module 25 .

[0045] The laser emitter 1 emits excitation light to the first dichroic mirror 3, and the firs...

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Abstract

The invention provides a color super-resolution imaging device and method, and relates to the technical field of microscopic imaging. The method includes: dividing the fluorescence signal of the sample to be tested through a first dichroic mirror to a beam splitter and dividing it into a first beam path and a second beam path; transmitting the first beam path to a CCD camera to collect a super-resolution image; The second beam path is emitted to the three-channel photomultiplier tube module, and the three color channel images of red, green and blue are collected respectively; the red, green, and blue color channel images are respectively subjected to matrix dot product with the super-resolution image to generate the red color respectively. The super-resolution image, the green color super-resolution image and the blue color super-resolution image; the red color super-resolution image, the green color super-resolution image and the blue color super-resolution image are subjected to matrix addition operation to generate a color super-resolution image. The invention solves the problems of low signal acquisition rate and efficiency existing in the current secondary scanning super-resolution microscopy technology.

Description

technical field [0001] The invention relates to the technical field of microscopic imaging, in particular to a color super-resolution imaging device and method. Background technique [0002] At present, in structured light illumination technology, in order to realize super-resolution microscopic imaging, it is necessary to collect multiple images of the sample to be tested, and after the acquisition, a complex image reconstruction process is performed to restore the super-resolution image of the sample to be tested. It can be seen that the traditional super-resolution microscopy imaging method is not only time-consuming, but also multiple acquisitions will further increase the chance of sample photobleaching. [0003] At present, there is a secondary scanning super-resolution microscopy technology, which is a new type of structured light illumination super-resolution microscopy technology, and its basic principle can be as follows: figure 1 shown. Among them, there are two...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 郑炜陈龙超
Owner SHENZHEN INST OF ADVANCED TECH
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