Rapid inducing medium device for tissue culture of Alhagi sparsifolia Shap

Abstract

The invention particularly relates to a rapid inducing medium device for tissue culture of Alhagi sparsifolia Shap. The rapid inducing medium device comprises a sterile box and an inducing medium located in the sterile box, wherein the sterile box comprises an upper cubic operation box and a lower funnel-shaped culture box fixed at the bottom of the upper operation box; the inducing medium is prepared by adding a special curing agent, brown sugar, 6-benzylaminopurine, 4-chloro-IAA (indole-3-acetic acid), tetracycline, activated carbon, plant extract and papaya juice into a 1/2MS medium. According to the rapid inducing medium device, the tetracycline is further added to the inducing medium and can have an obvious inhibiting effect on endophyte of an explant, further, by means of cooperation of ficus lacor aerial root extract and 4-chloro-IAA, growth of axillary buds, roots and stems of Alhagi sparsifolia Shap can be effectively balanced, and axillary bud germination and bud development are remarkably promoted. With the application of the inducing medium for culture of Alhagi sparsifolia Shap, the rate of survival is 98%-99%, the contamination rate is 1.7%-2.1%, the browning degree of the medium is 14%-17% lower than that of an MS medium, and the germinating time is shortened by 25%-35%.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a rapid induction medium device for camel thorn tissue culture. Background technique [0002] Camel thorn (scientific name: Alhagisparsifolia Shap.) belongs to the family Fabaceae, deciduous herb, with thorns on the main branches, oblong leaves, pink flowers, blooming in June, the most prosperous in August, each flower can be open for more than 20 days, pods, raceme Inflorescences, roots generally up to 20 m long. From desert It is a kind of drought-tolerant plant that grows naturally and absorbs underground water and nutrients deep in the Gobi Desert. Because the stem of this plant grows thorn-shaped very hard small green leaves, it is called camel thorn. It is a herbaceous plant. Camel grass is the only grass that camels can eat in the desert, so it is also called camel grass, which is mainly distributed in inland arid areas. [0003] The root system of...

AI Technical Summary

Problems solved by technology

Because of its special physiological characteristics, if the commonly used auxin indole acetic acid is added to the induction medium at a low concentration, it cannot quickly promote stem and leaf different

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