Lens-free microscope based on LED light source and image reconstruction method of microscope

Abstract

The invention discloses a lens-free microscope based on an LED light source and an image reconstruction method of the microscope. An LED light source, a pin hole, a sample table, and a camera are arranged one by one and form an imaging system. The LED light source is installed on the bottom of the whole imaging system, and a photosensitive surface of the LED light source is on the optical axis of the whole imaging system. The pin hole is close to and opposite to a light emitting surface of the LED light source. The LED light source is used as an illuminating light source of the microscope, and adopts a monochromatic LED with a central wavelength of lambda or RGB three-color LEDs. Firstly, LED is used as the illuminating light source of the microscope, and a needed light intensity image is captured; then phase information of a to-be-tested light wave field is obtained through phase recovery by means of an iterative method; and finally a computer is used to achieve inverse propagation of values and a focused image of an object under test is obtained. The microscope does not need any imaging optical element, so that the system structure is simplified, the size of the microscope is reduced, and the cost is lowered.

Description

technical field [0001] The invention belongs to optical microscopic imaging technology, in particular to a lensless microscope based on an LED light source and an image reconstruction method thereof. Background technique [0002] Optical microscopes have been the core instruments for biomedical detection and analysis since they were used for biomedical observation in the 1660s. The advent of phase contrast microscopes, differential interference phase contrast microscopes, fluorescence microscopes, and laser confocal microscopes has greatly promoted the improvement of life science research. They provide higher resolution and imaging quality for disease diagnosis, especially for major malignant diseases. Early diagnosis provides powerful imaging evidence and becomes an indispensable tool in modern clinical medicine. However, these microscope systems are still based on the "what you see is what you get" imaging method, and do not take full advantage of the powerful functions o...

AI Technical Summary

Problems solved by technology

Although this method omits the imaging lens, it introduces an additional expensive and bulky laser light source, which does not effectively reduce the size and cost of the system

In addition, the harsh requirements of the interferometry itself on the measurement environment, as well as the speckle noise introduced by the highly coherent light source also show that it is not an ideal way to achieve lensless microscopic imaging

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