Lens-free phase micro-tomography device based on color LED array illumination and image reconstruction method

Abstract

The invention discloses a lens-free phase micro-tomography device based on color LED array illumination and an image reconstruction method thereof. The lens-free phase micro-tomography device comprises an LED array, a sample stage and a camera which are sequentially arranged to form an imaging system, wherein the LED array is placed at the bottom of the whole imaging system, a photosensitive surface of a central LED pixel of the LED array is placed on an optical axis of the whole imaging system, and image reconstruction is achieved by using the lens-free phase micro-tomography device. Without the aid of any imaging optical elements, the lens-free phase micro-tomography device simplifies system structure, reduces microscope size, and greatly reduces cost; the lens-free phase micro-tomography device can obtain refractive index tomography images of samples, thereby achieving marker-free ''true three-dimensional imaging'' of biological cell samples, and significantly improving flexibility and versatility of the microscope.

Description

technical field [0001] The invention belongs to optical microscopic imaging technology, in particular to a lensless phase microscopic tomography device and an image reconstruction method based on color LED array illumination. Background technique [0002] Optical microscopes have been the core instruments for biomedical detection and analysis since they were used for biomedical observation in the 1760s. The advent of phase contrast microscopes, differential interference phase contrast microscopes, fluorescence microscopes, and laser confocal microscopes has greatly promoted the improvement of life science research. They provide higher resolution and imaging quality for disease diagnosis, especially for major malignant diseases. Early diagnosis provides powerful imaging evidence and becomes an indispensable tool in modern clinical medicine. However, these microscope systems are still based on the "what you see is what you get" imaging method, and do not take full advantage o...

AI Technical Summary

Problems solved by technology

Although this method omits the imaging lens, it introduces an additional expensive and bulky laser light source, which does not effectively reduce the size and cost of the system

In addition, the harsh requirements of the interferometry itself on the measurement environment, as well as the speckle noise introduced by the highly coherent light source also show that it is not an ideal way to achieve lensless microscopic imaging

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