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Bacillus amyloliquefaciens strain for control of crop greensickness and microbial agent thereof

A technology of amyloliquefaciens and microbial inoculants, applied in the application field of preventing and treating crop Verticillium wilt, can solve problems such as no reports of Bacillus amyloliquefaciens strain HMB27684 being found.

Active Publication Date: 2016-01-13
齐永新
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] After searching, no report about Bacillus amyloliquefaciens bacterial strain HMB27684 of the present invention was found

Method used

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  • Bacillus amyloliquefaciens strain for control of crop greensickness and microbial agent thereof
  • Bacillus amyloliquefaciens strain for control of crop greensickness and microbial agent thereof
  • Bacillus amyloliquefaciens strain for control of crop greensickness and microbial agent thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The preparation of embodiment 1HMB27684 microbial inoculum

[0057] Follow these steps:

[0058] (1) Activation of strains: Bacillus amyloliquefaciens strain HMB27684 (this strain has been preserved in the General Microbiology Center of China Microorganism Culture Collection Management Committee on September 29, 2015, the preservation number is CGMCCNo.11456) ) on the LB plate medium (its composition and its weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) at 30 ° C for activation, Pick a single colony on the LB slant medium (its composition and its weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) and cultivated at 30 ° C for 12 hours to obtain activated strains;

[0059] (2) Preparation of seed liquid: make LB liquid medium (its composition and its weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, water 1000mL) according to a conventional method...

Embodiment 2

[0062] Example 2 Inhibitory effect of Bacillus amyloliquefaciens HMB27684 on spore germination of Verticillium cotton

[0063] (1) Test time and place: In June 2013, it was carried out in the biological control laboratory.

[0064] (2) Test method:

[0065] (1) The source of Verticillium dahliae for the test: Verticillium dahliae WX-1 strain was collected from cotton Verticillium wilt diseased plants in Wei County, Xingtai City, Hebei Province, and was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry. The Department of Plant Pathology of the Conservation College identified it as Verticillium dahaliae, and the pathogenicity test showed that it was highly pathogenic.

[0066] (2) Plate measurement test:

[0067] Firstly, Verticillium spp. WX-1 was activated on a PDA plate, and after 7 days of culture, 4-5 mycelial blocks (irregular shape, about 15 mm) were picked from the edge area of ​​the colony. 2 ), inserted into the PD...

Embodiment 3

[0071] Example 3 Inhibitory effect test of Bacillus amyloliquefaciens HMB27684 on the growth of Verticillium versicolor mycelium

[0072] (1) Test time and place: in July 2013 in the biological control laboratory.

[0073] (2) Test method:

[0074] (1) The source of Verticillium dahliae for the test: Verticillium dahliae WX-1 strain was collected from cotton Verticillium wilt diseased plants in Wei County, Xingtai City, Hebei Province, and was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry. The Department of Plant Pathology of the Conservation College identified it as Verticillium dahaliae, and the pathogenicity test showed that it was highly pathogenic.

[0075] (2) Plate measurement test:

[0076]First, Verticillium cottonis WX-1 was activated on a PDA plate, and after culturing for 7 days, in the edge area of ​​the colony, with a hole punch ( ) Punching is made into bacterium sheet, the cotton Verticillium bacterium...

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Abstract

The invention belongs to the field of agricultural biological control, and particularly discloses a Bacillus amyloliquefaciens strain HMB27684 for control of crop greensickness, and the strain has a preservation number of CGMCC No.11456. The present invention also discloses a microbial agent prepared from the strain, and application thereof to control of crop greensickness. The invention provides an efficient microbe for the control of greensickness of cotton, eggplant and other crops, has average control effect of more than 70% on cotton and eggplant, and has strong pertinency; the microbial agent of the invention is safe to human and livestock, and has little environmental pollution; in addition, the strain has long effective period on the control of greensickness on cotton and eggplant, and does not produce drug resistance. The invention has the advantages of simple preparation method, low cost and easy usage.

Description

technical field [0001] The invention belongs to the field of agricultural biological control, in particular to a Bacillus amyloliquefaciens for preventing and treating crop verticillium wilt, a microbial inoculum containing the bacillus amyloliquefaciens, and their application in preventing and treating crop verticillium wilt. Background technique [0002] Verticillium wilt of cotton, eggplant and other crops caused by Verticillium dahaliae is a soil-borne disease that is seriously harmful and difficult to control. At present, the disease is becoming more and more serious in the main producing areas of cotton and eggplant in my country. Cotton verticillium wilt frequently breaks out in my country, and the cotton planting area affected by it is more than 40 million mu each year, which reduces cotton production by about 10% to 20% all the year round. %. Verticillium wilt often causes the whole eggplant to wilt and die, and it is almost impossible to harvest. [0003] Regardi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12Q1/68C12Q1/04C12R1/125
Inventor 李社增鹿秀云郭庆港张晓云
Owner 齐永新
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