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Aflatoxin B1 graphene oxide immunochromatographic test strip and application thereof

A technology for immunochromatographic test strips and aflatoxins, which is applied in analytical materials, measuring devices, instruments, etc., can solve the problems of reducing the cost of test strips, uniformity between batches and marketization, small batch output and high cost.

Active Publication Date: 2016-01-13
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many nano-gold immunochromatographic rapid detection products for single-component and multi-component aflatoxins on the market, but as a precious metal, nano-gold has the disadvantages of high cost, harsh preparation conditions, and low batch production. It is not conducive to the cost reduction, batch-to-batch uniformity and marketization of aflatoxin nano-gold immunochromatographic detection test strips

Method used

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  • Aflatoxin B1 graphene oxide immunochromatographic test strip and application thereof
  • Aflatoxin B1 graphene oxide immunochromatographic test strip and application thereof
  • Aflatoxin B1 graphene oxide immunochromatographic test strip and application thereof

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preparation example Construction

[0061] The preparation of carboxylated graphene oxide solution can adopt following method:

[0062] Pass 3.0g of flake graphite through a 325 mesh sieve and add it to 400mL of a mixed solution of sulfuric acid and phosphoric acid with a volume ratio of 9:1, and stir for 10 minutes; More, to avoid the reaction temperature exceeds 20 ℃. After all the potassium permanganate was added, it was heated to 50°C and stirred for 12 hours. After the reaction, let the mixture cool down to room temperature naturally, pour it into ~400mL of ice water, keep stirring at room temperature for 0.5 hours, add 30% hydrogen peroxide dropwise to the reaction system until the solution turns bright yellow and stops. Let it stand overnight, discard the supernatant, wash 3 times with 1L of hydrochloric acid with a mass fraction of 5%-10%, and then wash 5 times with 1L of deionized water to remove metal ions, sulfate ions and chloride ions, and finally the obtained The solid was freeze-dried, then plac...

Embodiment 1-2

[0064] Example 1-2: Preparation and application of graphene oxide immunochromatographic test strip for rapid detection of aflatoxin B1

Embodiment 1

[0066] The preparation method of rapid detection aflatoxin B1 graphene oxide immunochromatographic test strip comprises the following steps:

[0067] Preparation of chromatography test strips:

[0068] (1) Preparation of absorbent pad

[0069] Cut the absorbent paper to a size of 42mm and 3mm wide to obtain an absorbent pad;

[0070] (2) Preparation of detection pad

[0071] The coating of the detection line:

[0072] The aflatoxin B1-bovine serum albumin conjugate AFB1-BSA was prepared with coating buffer to make a coating solution of 0.7mg / mL; at a position 8mm away from the upper edge of the sample pad, it was coated horizontally by line spraying. Coated on a nitrocellulose membrane to obtain a detection line, the coating amount of aflatoxin B1-bovine serum albumin conjugate required per centimeter of detection line is 420ng, and then dried at 37°C for 45 minutes;

[0073] The coating buffer is: 1g bovine serum albumin, 0.02g sodium azide, 0.8g sodium chloride, 0.29g di...

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Abstract

The present invention relates to an aflatoxin B1 graphene oxide immunochromatographic test strip and application thereof. The aflatoxin B1 graphene oxide immunochromatographic test strip includes a chromatographic test strip and a carboxylated graphene oxide marked anti-aflatoxin B1 monoclonal antibody reaction reagent. The chromatographic test strip includes a cardboard; a water absorption pad, a detection pad and a sample pad are successively pasted on one side of the cardboard from top to bottom; adjacent pads are overlapped and connected at the junctions; the detection pad uses a nitrocellulose membrane as a base pad; the nitrocellulose membrane is provided with a horizontal quality control line and a test line from top to bottom; the quality control line is wrapped with rabbit antimouse polyclonal antibody; and the detection line is wrapped with an aflatoxin B1-bovine serum albumin conjugate (AFB1-BSA). The immunochromatographic test strip uses a carbon nano material as a marking material, which costs less than nano-gold and has high practical application value. The immunochromatographic test strip can be used for the detection of aflatoxin B1, and is easy to operate, simple and fast.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to an aflatoxin B1 graphene oxide immunochromatographic test strip and an application thereof. Background technique [0002] Aflatoxins are a class of toxic secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus infecting agricultural products and food. Since the first discovery of aflatoxins in 1960, as many as 20 types of aflatoxins have been discovered so far. Among them, aflatoxin B1 is the main type of contaminated plant agricultural products, and the most toxic, 10 times that of potassium cyanide, is a class I carcinogen. After the feed contaminated by aflatoxin B1 is eaten by animals, it can also be converted into M1 in meat, egg and milk in the animal body, contaminating the entire food chain. Therefore, the research on the detection technology of aflatoxin B1 is of great significance to the protection of human health and life safety...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 喻理李培武张奇张文丁小霞许琳朱建国
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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