Method for extracting plant calcium from soybean seedling
A technology for soybean seedlings and plant calcium, which is applied in the field of plant calcium extraction, can solve the problems of difficult absorption and easy formation of stones, and achieve the effects of good absorption effect, easy consumption, and high calcium ion content
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Embodiment 1
[0020] In an embodiment of the present invention, a method for extracting plant calcium from soybean seedlings consists of the following steps:
[0021] 1) First remove the damaged part of the collected fresh soybean seedlings, rinse them with clean water, soak them in 70% alcohol solution, then drain the alcohol solution on the sieve, put them in sterile water to wash away the alcohol solution , drain on a sieve. Repeat the wash with sterile water to ensure that no alcohol solution remains.
[0022] 2) In order to reduce the contamination of microorganisms, the soybean seedlings cleaned with alcohol are immediately sent to the sterile working room, crushed with a vegetable grinder, cellulase and pectinase are added to the crushed soybean seedlings, and the Keep the temperature for 20 minutes, and the added amount of cellulase and pectinase is 1% of the total mass of soybean seedlings.
[0023] 3) Add hydrochloric acid to the above enzyme-treated solution. The volume of hydr...
Embodiment 2
[0027] In an embodiment of the present invention, a method for extracting plant calcium from soybean seedlings consists of the following steps:
[0028] 1) First remove the damaged part of the collected fresh soybean seedlings, rinse them with clean water, soak them in 75% alcohol solution, then drain the alcohol solution on the sieve, put them in sterile water to wash away the alcohol solution , drain on a sieve. Repeat the wash with sterile water to ensure that no alcohol solution remains.
[0029] 2) In order to reduce the contamination of microorganisms, the soybean seedlings cleaned with alcohol are immediately sent to the sterile working room, crushed with a vegetable grinder, cellulase and pectinase are added to the crushed soybean seedlings, and the The temperature was maintained for 40 minutes, and the amount of cellulase and pectinase added was 5% of the total mass of soybean seedlings.
[0030] 3) Add hydrochloric acid to the above enzyme-treated solution. The vol...
Embodiment 3
[0034] In an embodiment of the present invention, a method for extracting plant calcium from soybean seedlings consists of the following steps:
[0035] 1) First remove the damaged part of the collected fresh soybean seedlings, rinse them with clean water, soak them in 72% alcohol solution, then drain the alcohol solution on the sieve, put them in sterile water to wash away the alcohol solution , drain on a sieve. Repeat the wash with sterile water to ensure that no alcohol solution remains.
[0036] 2) In order to reduce microbial contamination, the soybean seedlings cleaned with alcohol are immediately sent to the sterile working room, and crushed with a vegetable grinder. Cellulase and pectinase are added to the crushed soybean seedlings. The temperature was maintained for 30 minutes, and the amount of cellulase and pectinase added was 3% of the total mass of soybean seedlings.
[0037] 3) Add hydrochloric acid to the above enzyme-treated solution. The volume of hydrochlo...
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